AB322427

Human Papillomavirus Type 16 E7 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human Papillomavirus Type 16 E7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Papillomavirus Type 16 E7 with a sensitivity of 1.424 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

Protein E7, E7

3 Images

Sandwich ELISA - Human Papillomavirus Type 16 E7 ELISA Kit (AB322427)

sELISA

Supplier Data

Sandwich ELISA - Human Papillomavirus Type 16 E7 ELISA Kit (AB322427)

Recombinant Papillomavirus Type 16 E7 was spiked into the following human samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer SSW. Undiluted samples are 100 µg/mL A549 cell extract and 184 µg/mL human liver tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Papillomavirus Type 16 E7 ELISA Kit (AB322427)

Example of Human Papillomavirus Type 16 E7 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Papillomavirus Type 16 E7 ELISA Kit (AB322427)

Example of Human Papillomavirus Type 16 E7 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Assay type

Sandwich (quantitative)

Sensitivity

= 1.424 pg/mL

Range

4.688 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human Papillomavirus Type 16 E7 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Papillomavirus Type 16 E7 protein in Cell Lysate and Tissue extracts. Quantitate Human Papillomavirus Type 16 E7 with 1.42 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.3" } ]

style

left-column

Recovery

[ { "sample": "Cell Lysate", "range": "89 - 91 %", "average": "= 90" }, { "sample": "Tissue Extracts", "range": "90 - 96 %", "average": "= 94" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab322427-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB322427-CMP04" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB322427-CMP03" }, { "size":"2 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322427-CMP08" }, { "size":"10 x 600 µL", "name":"10X Human Papillomavirus Type 16 E7 Detector Antibody", "number":"AB322427-CMP09" }, { "size":"10 x 600 µL", "name":"10X Human Papillomavirus Type 16 E7 Capture Antibody", "number":"AB322427-CMP01" }, { "size":"20 x 1 Vial", "name":"Human Papillomavirus Type 16 E7 Lyophilized Recombinant Protein", "number":"AB322427-CMP10" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB322427-CMP06" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322427-CMP12" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322427-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB322427-CMP11" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab322427-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB322427-CMP04" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB322427-CMP03" }, { "size":"1 x 50 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322427-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human Papillomavirus Type 16 E7 Detector Antibody", "number":"AB322427-CMP09" }, { "size":"1 x 600 µL", "name":"10X Human Papillomavirus Type 16 E7 Capture Antibody", "number":"AB322427-CMP01" }, { "size":"2 x 1 Vial", "name":"Human Papillomavirus Type 16 E7 Lyophilized Recombinant Protein", "number":"AB322427-CMP10" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322427-CMP12" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB322427-CMP11" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB322427-CMP07" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322427-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab322427-1x96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB322427-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB322427-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB322427-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Papillomavirus Type 16 E7 Detector Antibody", "number":"AB322427-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Papillomavirus Type 16 E7 Capture Antibody", "number":"AB322427-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Papillomavirus Type 16 E7 Lyophilized Recombinant Protein", "number":"AB322427-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322427-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB322427-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322427-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322427-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322427-CMP06", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).

See full target information Protein E7

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com