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AB316911

Human Papillomavirus Type 18 E7 ELISA Kit

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Human Papillomavirus Type 18 E7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Papillomavirus Type 18 E7 with a sensitivity of 16.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Protein E7, E7

2 Images
Sandwich ELISA - Human Papillomavirus Type 18 E7 ELISA Kit (AB316911)
  • sELISA

Supplier Data

Sandwich ELISA - Human Papillomavirus Type 18 E7 ELISA Kit (AB316911)

Interpolated concentrations of species Human Papillomavirus Type 18 E7 in HeLa cell extract

Interpolated concentration of native Human Papillomavirus Type 18 E7 was measured in duplicate at different sample concentrations. Undiluted samples are 200 µg/mL HeLa cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Papillomavirus Type 18 E7 ELISA Kit (AB316911)
  • sELISA

Supplier Data

Sandwich ELISA - Human Papillomavirus Type 18 E7 ELISA Kit (AB316911)

Example of species Human Papillomavirus Type 18 E7 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 16.8 pg/mL

Range

50 - 3200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB308296

Anti-HPV18 E7 antibody [EPR26911-29]

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Flow Cytometry (Intracellular) - Anti-HPV18 E7 antibody [EPR26911-29] (AB308296)

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Proteins & Peptides

AB237790

Recombinant Human Papillomavirus 16 (E7) protein (His tag)

Mass Spectrometry - Recombinant Human Papillomavirus 16 (E7) protein (His tag) (AB237790)

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Assay Kits

AB300761

Human papillomavirus type 16 (E7) Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human papillomavirus type 16 (E7) Antibody Pair - BSA and Azide free (AB300761)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Papillomavirus Type 18 E7 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Papillomavirus Type 18 E7 protein in Cell Lysate. Quantitate  Human Papillomavirus Type 18 E7 with 16.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "100 - 101 %", "average": "= 101" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta) (PubMed : 26405230).
See full target information E7
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com