Human PAPP A2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human PAPP A2 in Human in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
0.2 - 150 ng/mL
- Sensitivity
= 0.23 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Metalloproteinase which specifically cleaves insulin-like growth factor binding protein (IGFBP)-5 at the '163-Ser-|-Lys-164' bond. Shows limited proteolysis toward IGFBP-3.
Alternative names
PLAC3, PLAC3, PAPPA2, Pappalysin-2, Pregnancy-associated plasma protein A2, Pregnancy-associated plasma protein E1, PAPP-A2, PAPP-E
What's included?
Recommended products
Human PAPP A2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human PAPP A2 in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
0.2 - 150 ng/mL
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 0.23 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 117.6 | Range 95 - 140 % |
Sample type Plasma | Average % = 132.1 | Range 127 - 140 % |
Sample type Cell culture extracts | Average % = 103 | Range 76 - 142 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Human PAPP A2 ELISA Kit (ab277446) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PAPP A2 in serum (human PAPP A2 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma and cell culture supernatants.
This assay employs an antibody specific for Human PAPP A2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PAPP A2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PAPP A2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PAPP A2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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1 product image
ELISA - Human PAPP A2 ELISA Kit (ab277446)
These standard curves are for demonstration only. A standard curve must be run with each assay.
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Product protocols
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