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AB277446

Human PAPP A2 ELISA Kit

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Human PAPP A2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PAPP A2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PLAC3, PAPPA2, Pappalysin-2, Pregnancy-associated plasma protein A2, Pregnancy-associated plasma protein E1, PAPP-A2, PAPP-E

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ELISA - Human PAPP A2 ELISA Kit (AB277446)
  • ELISA

Supplier Data

ELISA - Human PAPP A2 ELISA Kit (AB277446)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.23 ng/mL

Range

0.2 - 150 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PAPP A2 ELISA Kit (ab277446) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PAPP A2 in serum (human PAPP A2 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma and cell culture supernatants.

This assay employs an antibody specific for Human PAPP A2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PAPP A2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PAPP A2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PAPP A2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 140 %", "average": "= 117.6" }, { "sample": "Plasma", "range": "127 - 140 %", "average": "= 132.1" }, { "sample": "Cell culture extracts", "range": "76 - 142 %", "average": "= 103" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277446-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277446-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277446-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human PAPP A2 coated Microplate", "number":"AB277446-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human PAPP A2 Antibody", "number":"AB277446-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277446-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB277446-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277446-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB277446-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PAPP A2 standard protein (Lyophilized)", "number":"AB277446-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Metalloproteinase which specifically cleaves insulin-like growth factor binding protein (IGFBP)-5 at the '163-Ser-|-Lys-164' bond. Shows limited proteolysis toward IGFBP-3.
See full target information PAPPA2
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websiteProtocolBooklet
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