AB235647

Human PAPPA ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Human PAPPA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PAPPA with a sensitivity of 58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Pappalysin-1, Insulin-like growth factor-dependent IGF-binding protein 4 protease, Pregnancy-associated plasma protein A, IGF-dependent IGFBP-4 protease, IGFBP-4ase, PAPP-A, PAPPA

4 Images

Sandwich ELISA - Human PAPPA ELISA Kit (AB235647)

sELISA

Supplier Data

Sandwich ELISA - Human PAPPA ELISA Kit (AB235647)

Interpolated concentrations of native PAPPA in human placenta extract based on a 300 μg/mL extract load. The concentrations of PAPPA were measured in duplicate and interpolated from the PAPPA standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAPPA concentration was determined to be 13,470.7 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human PAPPA ELISA Kit (AB235647)

Example of human PAPPA standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human PAPPA ELISA Kit (AB235647)

Interpolated concentrations of native PAPPA in human pregnant serum. The concentrations of PAPPA were measured in duplicates, interpolated from the PAPPA standard curves and corrected for sample dilution. Undiluted samples are as follows : pregnant serum 1 : 167. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAPPA concentration was determined to be 1,390.617 ng/mL in pregnant serum.

sELISA

Supplier Data

Sandwich ELISA - Human PAPPA ELISA Kit (AB235647)

Example of human PAPPA standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 58 pg/mL

Range

187.5 - 16000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human PAPPA ELISA Kit (ab235647) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAPPA protein in cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAPPA with 8.89 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" } ]

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Recovery

[ { "sample": "Serum", "range": "106 - 126 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "82 - 103 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "94 - 103 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "95 - 96 %", "average": "= 96" }, { "sample": "Tissue Extracts", "range": "79 - 94 %", "average": "= 86" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235647-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235647-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB235647-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB235647-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235647-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB235647-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB235647-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB235647-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PAPPA Capture Antibody", "number":"AB235647-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PAPPA Lyophilized Recombinant Protein", "number":"AB235647-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB235647-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PAPPA Detector Antibody", "number":"AB235647-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235647-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.

See full target information Pappalysin-1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proteomics 19:e1800164 PubMed30536821

2018

Quantitative Proteomics by SWATH-MS Suggest an Association Between Circulating Exosomes and Maternal Metabolic Changes in Gestational Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Nanthini Jayabalan,Andrew Lai,Soumyalekshmi Nair,Dominic Guanzon,Katherin Scholz-Romero,Carlos Palma,Harold David McIntyre,Martha Lappas,Carlos Salomon

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PAPPA ELISA Kit

Sandwich ELISA - Human PAPPA ELISA Kit (AB235647)

Sandwich ELISA - Human PAPPA ELISA Kit (AB235647)

Sandwich ELISA - Human PAPPA ELISA Kit (AB235647)

Sandwich ELISA - Human PAPPA ELISA Kit (AB235647)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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