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AB277440

Human PAR1/Thrombin Receptor ELISA Kit

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Human PAR1/Thrombin Receptor ELISA Kit is a Sandwich ELISA for the measurement of Human PAR1/Thrombin Receptor in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CF2R, PAR1, TR, F2R, Proteinase-activated receptor 1, PAR-1, Coagulation factor II receptor, Thrombin receptor

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ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB277440)
  • ELISA

Supplier Data

ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB277440)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3 pg/mL

Range

2.87 - 700 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PAR1/Thrombin Receptor ELISA Kit (ab277440) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PAR1/Thrombin Receptor in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human PAR1/Thrombin Receptor coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PAR1/Thrombin Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PAR1/Thrombin Receptor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PAR1/Thrombin Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "100 - 110 %", "average": "= 105.3" }, { "sample": "Plasma", "range": "103 - 125 %", "average": "= 113.2" }, { "sample": "Cell culture media", "range": "115 - 133 %", "average": "= 123" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277440-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277440-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277440-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human PAR1 Thrombin Receptor coated Microplate", "number":"AB277440-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PAR1/Thrombin Receptor standard protein (Lyophilized)", "number":"AB277440-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human PAR1/Thrombin Receptor Antibody", "number":"AB277440-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277440-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277440-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277440-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277440-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores (PubMed : 1672265, PubMed : 8136362). The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores (By similarity). In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). May play a role in platelets activation and in vascular development (PubMed : 10079109). Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells (PubMed : 30568593, PubMed : 34831181).
See full target information F2R
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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