AB283544

Human PAR1/Thrombin Receptor ELISA Kit

Recombinant
SimpleStep
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Human PAR1/Thrombin Receptor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PAR1/Thrombin Receptor with a sensitivity of 1.21 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CF2R, PAR1, TR, F2R, Proteinase-activated receptor 1, PAR-1, Coagulation factor II receptor, Thrombin receptor

3 Images

Sandwich ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB283544)

sELISA

Supplier Data

Sandwich ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB283544)

Interpolated concentrations of native PAR1/Thrombin Receptor in human HT-29 cell extract based on a 100 μg/mL extract load.

The concentrations of PAR1/Thrombin Receptor were measured in duplicate and interpolated from the PAR1/Thrombin Receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAR1/Thrombin Receptor concentration was determined to be 236.37 pg/mL in HT-29 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB283544)

Interpolated concentrations of native PAR1/Thrombin Receptor in human serum, plasma and cell culture supernatant samples.

The concentrations of PAR1/Thrombin Receptor were measured in duplicates, interpolated from the PAR1/Thrombin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 100%, HT-29 supernatant 50%, and A375 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAR1/Thrombin Receptor concentration was determined to be 420.97 pg/mL in serum, 464.59 pg/mL in plasma (EDTA), 97.80 pg/mL in plasma (heparin), 360.01 pg/mL in HT-29 supernatant and 28.52 pg/mL in A375 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB283544)

Example of human PAR1/Thrombin Receptor standard curve in Sample Diluent NS.

The PAR1/Thrombin Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.21 pg/mL

Range

3.125 - 200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human PAR1/Thrombin Receptor ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PAR1/Thrombin Receptor protein in human serum, plasma, cell culture supernatant, and cell culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAR1/Thrombin Receptor with 1.21 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human PAR1/Thrombin Receptor also known as Protease-activated receptor 1 or coagulation factor II (thrombin) receptor is a protein that in humans is encoded by the F2R gene. Human PAR1 is a G protein-coupled receptor and one of four protease-activated receptors involved in the regulation of thrombotic response. Highly expressed in platelets and endothelial cells, human PAR1 plays a key role in mediating the interplay between coagulation and inflammation, which is important in the pathogenesis of inflammatory and fibrotic lung diseases. Human PAR1 is also involved both in disruption and maintenance of endothelial barrier integrity, through interaction with either thrombin or activated protein C, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "107 - 113 %", "average": "= 110" }, { "sample": "Serum", "range": "106 - 116 %", "average": "= 113" }, { "sample": "EDTA Plasma", "range": "107 - 112 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "95 - 101 %", "average": "= 98" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283544-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283544-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283544-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB283544-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PAR1/Thrombin Receptor Lyophilized Recombinant Protein", "number":"AB283544-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283544-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PAR1/Thrombin Receptor Capture Antibody", "number":"AB283544-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PAR1/Thrombin Receptor Detector Antibody", "number":"AB283544-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283544-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283544-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB283544-CMP13", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB283544-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283544-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores (PubMed : 1672265, PubMed : 8136362). The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores (By similarity). In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). May play a role in platelets activation and in vascular development (PubMed : 10079109). Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells (PubMed : 30568593, PubMed : 34831181).

See full target information F2R

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human PAR1/Thrombin Receptor ELISA Kit

Sandwich ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB283544)

Sandwich ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB283544)

Sandwich ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB283544)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

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Product promise