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AB313932

Human Parathyroid Hormone Receptor 1/PTH1R ELISA Kit

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Human Parathyroid Hormone Receptor 1/PTH1R ELISA Kit is a Sandwich ELISA for the measurement of Human Parathyroid Hormone Receptor 1/PTH1R in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

PTHR, PTHR1, PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor, PTH/PTHrP type I receptor, Parathyroid hormone 1 receptor, PTH/PTHr receptor, PTH1 receptor

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Sandwich ELISA - Human Parathyroid Hormone Receptor 1/PTH1R ELISA Kit (AB313932)
  • sELISA

Supplier Data

Sandwich ELISA - Human Parathyroid Hormone Receptor 1/PTH1R ELISA Kit (AB313932)

Example of standard curve using ab313932. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8.2 pg/mL

Range

8.19 - 2000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Parathyroid Hormone Receptor 1/PTH1R ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Parathyroid Hormone Receptor 1/PTH1R in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Parathyroid Hormone Receptor 1/PTH1R coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "93 - 121 %", "average": "= 110" }, { "sample": "Plasma", "range": "82 - 122 %", "average": "= 100.9" }, { "sample": "Cell culture media", "range": "74 - 116 %", "average": "= 100.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313932-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human Hormone Receptor 1/PTH1R Antibody-coated ELISA Plate", "number":"AB313932-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313932-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Hormone Receptor 1/PTH1R Protein Standard", "number":"AB313932-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Hormone Receptor 1/PTH1R Detection Antibody", "number":"AB313932-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB313932-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313932-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313932-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313932-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313932-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid Hormone Receptor 1 (PTH1R) also known as the parathyroid receptor or hormone receptor is a G-protein-coupled receptor with an approximate molecular mass of 66 kDa. This receptor is found in various tissues mainly in bone and kidney. PTH1R interacts with parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHrP) both of which play significant roles in maintaining calcium homeostasis. The receptor is an important regulator in these processes facilitating the signal transduction that governs important physiological responses.
Biological function summary

PTH1R facilitates the regulation of calcium and phosphate balance in the body. It does not act alone but participates as a part of a signaling complex engaging with intracellular G-proteins to initiate signaling cascades. The receptor's activity helps modulate bone remodeling by affecting osteoblast and osteoclast activity which are essential for bone development and mineralization. Moreover in the kidney PTH1R adjusts calcium reabsorption and phosphate excretion supporting the maintenance of serum calcium levels.

Pathways

PTH1R plays a significant role in the cyclic AMP (cAMP) and phosphatidylinositol signaling pathways. When PTH or PTHrP binds to PTH1R it activates the adenylate cyclase increasing cAMP levels and subsequently activating protein kinase A (PKA). This pathway affects various physiological processes like gene expression and metabolic regulation. In addition PTH1R interacts with the phospholipase C pathway facilitating calcium mobilization inside cells. Proteins like Gs and Gq are involved in these pathways aiding in the receptor's ability to exert its biological effects.

PTH1R is associated with conditions such as osteoporosis and hypoparathyroidism. Osteoporosis involves abnormal bone loss and malfunctioning of the receptor can exacerbate this condition by impairing normal bone remodeling. In hypoparathyroidism insufficient signaling through PTH1R leads to low levels of calcium affecting bone and other bodily functions. The receptor's interaction with proteins such as parathyroid hormone-related peptide further extends its relevance to these conditions as alterations in these proteins can influence disease progression.
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Product protocols

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Target data

G-protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH) (PubMed : 10913300, PubMed : 18375760, PubMed : 19674967, PubMed : 27160269, PubMed : 30975883, PubMed : 35932760, PubMed : 8397094). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP) (PubMed : 30975883, PubMed : 35932760). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed : 20172855, PubMed : 30975883, PubMed : 35932760). PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH (PubMed : 35932760).
See full target information PTH1R
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Product promise

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