AB282293

Human Parvalbumin ELISA Kit

Recombinant
SimpleStep
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Human Parvalbumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Parvalbumin with a sensitivity of 1.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Parvalbumin alpha, PVALB

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Sandwich ELISA - Human Parvalbumin ELISA Kit (AB282293)

sELISA

Supplier Data

Sandwich ELISA - Human Parvalbumin ELISA Kit (AB282293)

Example of human Parvalbumin standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

The Parvalbumin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Parvalbumin ELISA Kit (AB282293)

Serum from eight individual healthy female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean detectable Parvalbumin concentration was determined to be 28.7 pg/mL with a range of not detected – 112 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Parvalbumin ELISA Kit (AB282293)

Interpolated concentrations of native Parvalbumin in human brain extract based on a 25 μg/mL extract load.

The concentrations of Parvalbumin were measured in duplicate and interpolated from the Parvalbumin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Parvalbumin ELISA Kit (AB282293)

Interpolated concentrations of native Parvalbumin in human serum and plasma (Heparin).

The concentrations of Parvalbumin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma - heparin, 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 82 pg/mL in serum and 83 pg/mL in plasma (Heparin).

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell culture media, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.1 pg/mL

Range

6.3 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Parvalbumin ELISA kit (ab282293) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Parvalbumin protein in human serum, plasma, cell culture supernatant, and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Parvalbumin with 1.1 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Parvalbumin alpha is a calcium-binding protein encoded by the PVALB gene. Parvalbumin is a small, 110- amino acid long, protein that contains three EF hand motifs, making it structurally related to calmodulin and troponin-C. Parvalbumin is found intracellularly in fast-contracting muscles where it is involved in muscle fiber relaxation. Also found in neural tissues, parvalbumin is present in GABAergic interneurons and is expressed predominantly by chandelier and basket cells. Serum parvalbumin is thought to be an indicator of muscle disease.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Serum", "range": "96 - 109 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "97 - 108 %", "average": "= 103" }, { "sample": "Tissue Extracts", "range": "104 - 114 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "97 - 98 %", "average": "= 98" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Parvalbumin sometimes referred to as PV is a calcium-binding protein approximately 12 kDa in size. It is recognized for its roles in muscle and neuronal tissues. Within the nervous system parvalbumin is prominently expressed in fast-spiking GABAergic interneurons while in muscle tissues it is present in fast-twitch muscle fibers. Other popular descriptors include anti PV and parvalbumins. These proteins play important roles in the regulation of cellular calcium levels.

Biological function summary

Parvalbumin helps modulate calcium ion concentration which is key in muscle relaxation and neurotransmission. It acts by buffering calcium ions and is not part of a larger protein complex. By impacting calcium transients parvalbumin affects processes like synaptic plasticity and the rapid contractions of muscle fibers. This is especially significant in the brain where rapid neuronal firing is needed and in muscles where quick recovery is essential.

Pathways

Parvalbumin is intimately linked with calcium signaling pathways as well as the regulation of muscle contraction. It ensures proper calcium ion homeostasis thereby aiding muscle relaxation after contraction. In the nervous system calbindin proteins parallel some of parvalbumin's functions participating in calcium ion buffering and modulation of neuron excitability. The balanced action of these proteins helps maintain cellular functions in both neurons and muscles.

Insufficient parvalbumin expression may link to epilepsy and schizophrenia. Deficits in this protein could interfere with normal neurotransmission and muscle relaxation cycles. Parvalbumin deficiency influences neurotransmitter release affecting synaptic transmission which may exacerbate symptoms in these conditions. In disease mechanisms proteins such as calbindin may indirectly relate with parvalbumin showing similar patterns of dysregulation and compensating for its absence in calcium handling.

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Product protocols

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Target data

In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.

See full target information PVALB

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Parvalbumin ELISA Kit

Sandwich ELISA - Human Parvalbumin ELISA Kit (AB282293)

Sandwich ELISA - Human Parvalbumin ELISA Kit (AB282293)

Sandwich ELISA - Human Parvalbumin ELISA Kit (AB282293)

Sandwich ELISA - Human Parvalbumin ELISA Kit (AB282293)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise