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AB313919

Human Pax4 ELISA Kit

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Human Pax4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Pax4 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Paired box protein Pax-4, PAX4

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Sandwich ELISA - Human Pax4 ELISA Kit (AB313919)
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Supplier Data

Sandwich ELISA - Human Pax4 ELISA Kit (AB313919)

Example of standard curve using ab313919. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.14 ng/mL

Range

0.14 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Pax4 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Pax4 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Pax4 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "72 - 138 %", "average": "= 101.1" }, { "sample": "Plasma", "range": "72 - 138 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "86 - 92 %", "average": "= 88.43" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313919-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313919-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313919-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Pax4 Antibody-coated ELISA Plate", "number":"AB313919-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Pax-4 Protein Standard", "number":"AB313919-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Pax4 Detection Antibody", "number":"AB313919-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313919-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB313919-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313919-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313919-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX4 also known as paired box 4 is a protein encoded by the PAX4 gene. This transcription factor has a molecular mass of around 36 kDa. It plays a significant role in the regulation of pancreatic islet development and insulin gene expression. PAX4 is expressed mainly in pancreatic beta cells. Its expression is important for the proper differentiation of these cells from precursor cells and is pivotal during embryogenesis in the formation of pancreatic endocrine cells.
Biological function summary

PAX4 influences cellular activities critical for the development of insulin-producing cells in the pancreas. As a transcription factor it does not work in isolation but interacts with other proteins taking part in complexes that drive gene expression changes. It represses the expression of genes like glucagon ensuring the adequate development and function of insulin-secreting cells. Therefore PAX4 ensures a delicate balance in glucose homeostasis.

Pathways

PAX4 plays a significant part in the insulin signaling pathway and is essential in pathways affecting glucose metabolism. It interacts with RXR and PDX1 which are key proteins in the regulation of insulin and glucose metabolism genes. By controlling these pathways PAX4 contributes to maintaining normal glucose levels and effective insulin function which is critical for energy balance in the body.

Mutations or dysregulation of PAX4 have been linked to type 2 diabetes and maturity-onset diabetes of the young (MODY). These conditions result from the disrupted ability of pancreatic beta cells to produce insulin. PAX4 mutations can affect its interaction with proteins like PDX1 further influencing diabetes pathogenicity. Understanding PAX4 in these contexts can be vital for developing therapeutic strategies that target these pathways and their associated disorders.
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Product protocols

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Target data

Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.
See full target information PAX4
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB101721

Anti-PAX4 antibody

Western blot - Anti-PAX4 antibody (AB101721)

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