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AB184866

Human PBK ELISA Kit

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Human PBK ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PBK with a sensitivity of 133 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TOPK, PBK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, T-LAK cell-originated protein kinase, CT84, SPK

4 Images
Sandwich ELISA - Human PBK ELISA Kit (AB184866)
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Supplier Data

Sandwich ELISA - Human PBK ELISA Kit (AB184866)

Example of PBK standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PBK ELISA Kit (AB184866)
  • sELISA

Supplier Data

Sandwich ELISA - Human PBK ELISA Kit (AB184866)

Quantitation of PBK expression in different cell lines.

Interpolated values of PBK are plotted for the indicated cell lines based on a extract load of 250 μg/mL.

Sandwich ELISA - Human PBK ELISA Kit (AB184866)
  • sELISA

Supplier Data

Sandwich ELISA - Human PBK ELISA Kit (AB184866)

Interpolated concentrations of PBK in A431 extract.

The concentrations of PBK protein were interpolated from the PBK standard curve and corrected for sample dilution relative to extract load of 35.7 μg/mL. The mean PBK concentration was determined to be 6.95 ng/mL in A431 extract at 35.7 μg/mL.

Sandwich ELISA - Human PBK ELISA Kit (AB184866)
  • sELISA

Supplier Data

Sandwich ELISA - Human PBK ELISA Kit (AB184866)

Interpolated concentrations of PBK in MCF7 extract.

The concentrations of PBK protein were interpolated from the PBK standard curve and plotted against the extract load.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Suspension cells, Tissue Extracts, Tissue Homogenate, Adherent cells, Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 133 pg/mL

Range

300 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PBK (PDZ Binding Kinase) ELISA kit (ab184866) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PBK protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human PDZ Binding Kinase with 133 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

PDZ-binding kinase (also known PBK or Lymphocyte-activated killer T-cell-originated protein kinase) is a Serine/Threonine protein kinase in the MAP kinase kinase subfamily. PBK phosphorylates MAP kinase p38. PBK itself is phosphorylated and appears to be active only during the mitotic phase of the cell cycle. When phosphorylated PBK may interact with TP53. PBK may play a role in the activation of lymphoid cells and is found to expressed in many highly proliferative cell lines.

The standard is affinity purified.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Jurkat", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14" }, { "reproducibilityType": "Intra", "sample": "Jurkat", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "97 - 109 %", "average": "= 103" }, { "sample": "Extraction Buffer", "range": "99 - 100 %", "average": "= 100" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDZ Binding Kinase (PBK) also known as TOPK is a serine/threonine kinase with a molecular mass of approximately 36 kDa. It performs phosphorylation on certain proteins influencing their function localization or interaction with other proteins. PBK locates mainly in the testes placenta and various tumors but also expresses in smaller amounts in normal tissues such as the liver and brain. The kinase is cell cycle-dependent with activity peaking during mitosis where it plays a role by interacting with the mitotic spindle apparatus.
Biological function summary

PBK acts as part of cellular proliferation and differentiation processes. It can form complexes with other proteins enhancing its function and stability. The kinase significantly contributes to cell growth mainly in rapidly dividing cells. PBK influences processes like spermatogenesis in testes and placental development. PBK stabilizes certain cyclins and links with proteins such as p38 MAP kinase showing a role in stress response pathways.

Pathways

PBK integrates into the mitogen-activated protein kinase (MAPK) pathway and interacts with the tumor progression pathway. These pathways are critical for transmitting signals from the surface to the nucleus affecting cell fate decisions. PBK connects with proteins like JNK and extracellular signal-regulated kinase (ERK) displaying its importance in transmitting stress and growth signals. These interactions place PBK as a node linking growth factors and extracellular signals to cellular responses.

PBK is significantly associated with cancer development and progression such as in prostate cancer and glioma. In these conditions PBK often exhibits overexpression facilitating enhanced proliferation and survival of tumor cells. The connection to proteins like Cdc2/cyclin B1 promotes unchecked cell cycle progression often found in neoplasms. PBK's role in these disorders makes it a potential biomarker or therapeutic target in oncology.
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Product protocols

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Target data

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
See full target information PBK
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB236872

Anti-PBK/SPK antibody [EPR21983]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21983] (AB236872)

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Proteins & Peptides

AB85275

Recombinant human PBK/SPK protein

Functional Studies - Recombinant human PBK/SPK protein (AB85275)

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