AB196270

Human PCNA ELISA Kit

SimpleStep
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(3 Publications)

Human PCNA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PCNA with a sensitivity of 0.103 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Proliferating cell nuclear antigen, PCNA, Cyclin

4 Images

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

sELISA

Supplier Data

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

Representative image of Human PCNA ELISA Kit (ab196270)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human PCNA Capture Antibody

- 10X Human PCNA Detector Antibody

- Human PCNA Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

Example of PCNA standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

Titration of HeLa extracts treated with cell cycle arresting compounds and titrated within the working range of the assay.

HeLa cells were untreated asynchronous culture and 10 μM Etoposide, 24 hr (S/G2 arrest). PCNA levels were expected to peak during DNA replication in S-phase and return back to normal shortly after exiting S-phase. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

Bar graph displays interpolated values of PCNA of Cell extracts diluted to within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.103 ng/mL

Range

0.78 - 50 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human PCNA ELISA Kit (ab196270) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PCNA protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PCNA with 0.103 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Proliferating cell nuclear antigen (PCNA) is a 28.7 kDa protein which is an essential component of DNA replication. PCNA protein forms a homotrimeric ring structure which assembles around DNA forming a processivity sliding clamp. Levels of PCNA protein are low in quiescent cells and increase upon mitogen stimulation with peak protein levels localized to the nucleus during S-phase. PCNA interacts with a large number of proteins involved in DNA replication, cell cycle control, and cell cycle check point control.The standard is affinity purified.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "HeLa Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.9" }, { "reproducibilityType": "Intra", "sample": "HeLa Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.53" } ]

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Recovery

[ { "sample": "Cell culture media", "range": "103 - 110 %", "average": "= 106" }, { "sample": "Fetal Bovine Serum", "range": "108 - 115 %", "average": "= 111" }, { "sample": "Serum", "range": "89 - 116 %", "average": "= 99" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab196270-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB196270-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB196270-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB196270-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB196270-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB196270-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB196270-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PCNA Capture Antibody", "number":"AB196270-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PCNA Lyophilized Recombinant Protein", "number":"AB196270-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB196270-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PCNA Detector Antibody", "number":"AB196270-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB196270-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB196270-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.

Biological function summary

This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.

Pathways

PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.

PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.

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Product protocols

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Target data

Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed : 35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed : 24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair : Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed : 24695737).

See full target information PCNA

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 28: PubMed38067602

2023

Resistomycin Suppresses Prostate Cancer Cell Growth by Instigating Oxidative Stress, Mitochondrial Apoptosis, and Cell Cycle Arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Abeer S Aloufi,Ola A Habotta,Mohamed S Abdelfattah,Marina N Habib,Mohamed M Omran,Sally A Ali,Ahmed E Abdel Moneim,Shereen M Korany,Aisha M Alrajhi

Frontiers in immunology 13:1057746 PubMed36405726

2022

NLRP3+ macrophages aggravate inflammatory cystitis in diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Yubing Peng,Yan Gao

Scientific data 4:170091 PubMed28722704

2017

Targeted proteomic assays for quantitation of proteins identified by proteogenomic analysis of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ehwang Song,Yuqian Gao,Chaochao Wu,Tujin Shi,Song Nie,Thomas L Fillmore,Athena A Schepmoes,Marina A Gritsenko,Wei-Jun Qian,Richard D Smith,Karin D Rodland,Tao Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PCNA ELISA Kit

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

Sandwich ELISA - Human PCNA ELISA Kit (AB196270)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Product promise