Human PCNA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PCNA in Cell culture extracts, Tissue Extracts samples.
Images
Publications
- Molecules (Basel, Switzerland) 28:2023Resistomycin Suppresses Prostate Cancer Cell Growth by Instigating Oxidative Stress, Mitochondrial Apoptosis, and Cell Cycle Arrest.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbeer S Aloufi,Ola A Habotta,Mohamed S Abdelfattah,Marina N Habib,Mohamed M Omran,Sally A Ali,Ahmed E Abdel Moneim,Shereen M Korany,Aisha M AlrajhiPubMed 38067602
- Frontiers in immunology 13:10577462022NLRP3+ macrophages aggravate inflammatory cystitis in diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYubing Peng,Yan GaoPubMed 36405726
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
0.78 - 50 ng/mL
- Assay time
1h 30m
- Sensitivity
= 0.103 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).
Alternative names
Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
What's included?
Recommended products
Human PCNA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PCNA in Cell culture extracts, Tissue Extracts samples.
Alternative names
Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture extracts, Tissue Extracts
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
0.78 - 50 ng/mL
- Assay time
1h 30m
- Assay Platform
Microplate
- Sensitivity
= 0.103 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample HeLa Extract | n 8 | mean - | SD - | C.V. 6.53 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample HeLa Extract | n 3 | mean - | SD - | C.V. 10.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture media | Average % = 106 | Range 103 - 110 % |
Sample type Fetal Bovine Serum | Average % = 111 | Range 108 - 115 % |
Sample type Serum | Average % = 99 | Range 89 - 116 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human PCNA ELISA Kit (ab196270) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PCNA protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PCNA with 0.103 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Proliferating cell nuclear antigen (PCNA) is a 28.7 kDa protein which is an essential component of DNA replication. PCNA protein forms a homotrimeric ring structure which assembles around DNA forming a processivity sliding clamp. Levels of PCNA protein are low in quiescent cells and increase upon mitogen stimulation with peak protein levels localized to the nucleus during S-phase. PCNA interacts with a large number of proteins involved in DNA replication, cell cycle control, and cell cycle check point control.The standard is affinity purified.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- Activity summary
PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
- Biological function summary
This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.
- Pathways
PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.
- Associated diseases and disorders
PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Human PCNA ELISA Kit (ab196270)
Example of PCNA standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PCNA ELISA Kit (ab196270)
Bar graph displays interpolated values of PCNA of Cell extracts diluted to within the working range of the assay.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Sandwich ELISA - Human PCNA ELISA Kit (ab196270)
Titration of HeLa extracts treated with cell cycle arresting compounds and titrated within the working range of the assay.
HeLa cells were untreated asynchronous culture and 10 μM Etoposide, 24 hr (S/G2 arrest). PCNA levels were expected to peak during DNA replication in S-phase and return back to normal shortly after exiting S-phase. Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com