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Human PCOLCE ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human PCOLCE in Human in Cell culture supernatant, Plasma, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

51 - 12500 pg/mL

Sensitivity

= 51 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.C-terminal processed part of PCPE (CT-PCPE) may have an metalloproteinase inhibitory activity.

Alternative names

What's included?

1 x 96 Tests
Components
150X HRP-Streptavidin
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent (Item E2)
1 x 15 mL
Biotinylated Human PCOLCE Detection Antibody
2 x 1 Vial
ELISA Microplate Bag
1 x 1 Unit
Human PCOLCE Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human PCOLCE Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Human PCOLCE ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human PCOLCE in Human in Cell culture supernatant, Plasma, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

51 - 12500 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 51 pg/mL

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 122.3

Range

108 - 137 %

Sample type

Plasma

Average %

= 109.8

Range

93 - 118 %

Sample type

Cell culture media

Average %

= 130

Range

116 - 141 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human PCOLCE ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PCOLCE in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human PCOLCE coated on a 96-well plate.

Supplementary info

Biological function summary

PCOLCE serves as an enhancer of enzyme activity important for proper collagen maturation. This protein strongly associates with the biosynthesis of collagen fibrils being part of the larger complex responsible for proper collagen assembly. The effective regulation of collagen fibrils is essential for maintaining structural integrity and strength of various tissues such as the skin and bone matrix.

Activity summary

PCOLCE known as Procollagen C-endopeptidase enhancer is a glycoprotein with an approximate mass of 55 kDa. It resides mainly in the extracellular matrix playing a role in collagen processing. PCOLCE enhances the activity of the enzyme procollagen C-proteinase which is critical for converting procollagen to collagen by cleaving its C-terminal propeptide. PCOLCE expression occurs in tissues such as skin tendons and cartilage where collagen is abundant.

Pathways

PCOLCE plays an important role in the collagen biosynthesis and modifying pathways. It collaborates closely with other proteins like BMP1 (Bone Morphogenetic Protein 1) which also participates in the procollagen processing activity. Through its precise regulation PCOLCE affects tissue repair and development pathways ensuring efficient collagen synthesis and deposition critical for functional extracellular matrix formation.

Associated diseases and disorders

Mutations or dysregulation in PCOLCE can potentially relate to connective tissue disorders such as Ehlers-Danlos syndrome. In these conditions improper collagen processing occurs affecting tissue elasticity and strength. Studies suggest that abnormal PCOLCE activity may also influence fibrosis where excessive collagen deposition leads to tissue and organ dysfunction. In these contexts PCOLCE interaction with proteins like BMP1 and other collagen-modifying enzymes is significant affecting the progression of such disorders.

Product promise

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1 product image

  • Sandwich ELISA - Human PCOLCE ELISA Kit (ab313921), expandable thumbnail

    Sandwich ELISA - Human PCOLCE ELISA Kit (ab313921)

    Example of standard curve using ab313921. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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