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AB313921

Human PCOLCE ELISA Kit

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Human PCOLCE ELISA Kit is a Sandwich ELISA for the measurement of Human PCOLCE in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

PCPE1, PCOLCE, Procollagen C-endopeptidase enhancer 1, Procollagen COOH-terminal proteinase enhancer 1, Type 1 procollagen C-proteinase enhancer protein, Type I procollagen COOH-terminal proteinase enhancer, PCPE-1, Procollagen C-proteinase enhancer 1

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Sandwich ELISA - Human PCOLCE ELISA Kit (AB313921)
  • sELISA

Supplier Data

Sandwich ELISA - Human PCOLCE ELISA Kit (AB313921)

Example of standard curve using ab313921. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 51 pg/mL

Range

51 - 12500 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB243024

Anti-PCOLCE antibody [CL6567]

Western blot - Anti-PCOLCE antibody [CL6567] (AB243024)

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Proteins & Peptides

AB202191

Recombinant Human PCOLCE protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human PCOLCE protein (denatured) (His tag N-Terminus) (AB202191)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PCOLCE ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PCOLCE in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human PCOLCE coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "108 - 137 %", "average": "= 122.3" }, { "sample": "Plasma", "range": "93 - 118 %", "average": "= 109.8" }, { "sample": "Cell culture media", "range": "116 - 141 %", "average": "= 130" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313921-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human PCOLCE Antibody-coated ELISA Plate", "number":"AB313921-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313921-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313921-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313921-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human PCOLCE Detection Antibody", "number":"AB313921-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human PCOLCE Protein Standard", "number":"AB313921-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313921-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB313921-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB313921-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCOLCE known as Procollagen C-endopeptidase enhancer is a glycoprotein with an approximate mass of 55 kDa. It resides mainly in the extracellular matrix playing a role in collagen processing. PCOLCE enhances the activity of the enzyme procollagen C-proteinase which is critical for converting procollagen to collagen by cleaving its C-terminal propeptide. PCOLCE expression occurs in tissues such as skin tendons and cartilage where collagen is abundant.
Biological function summary

PCOLCE serves as an enhancer of enzyme activity important for proper collagen maturation. This protein strongly associates with the biosynthesis of collagen fibrils being part of the larger complex responsible for proper collagen assembly. The effective regulation of collagen fibrils is essential for maintaining structural integrity and strength of various tissues such as the skin and bone matrix.

Pathways

PCOLCE plays an important role in the collagen biosynthesis and modifying pathways. It collaborates closely with other proteins like BMP1 (Bone Morphogenetic Protein 1) which also participates in the procollagen processing activity. Through its precise regulation PCOLCE affects tissue repair and development pathways ensuring efficient collagen synthesis and deposition critical for functional extracellular matrix formation.

Mutations or dysregulation in PCOLCE can potentially relate to connective tissue disorders such as Ehlers-Danlos syndrome. In these conditions improper collagen processing occurs affecting tissue elasticity and strength. Studies suggest that abnormal PCOLCE activity may also influence fibrosis where excessive collagen deposition leads to tissue and organ dysfunction. In these contexts PCOLCE interaction with proteins like BMP1 and other collagen-modifying enzymes is significant affecting the progression of such disorders.
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Product protocols

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Target data

Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.. C-terminal processed part of PCPE (CT-PCPE) may have an metalloproteinase inhibitory activity.
See full target information PCOLCE
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websiteProtocolBooklet
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Product promise

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