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Human PD-1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PD-1 in Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Alternative names=Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1
Colorimetric
Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
25 - 1600 pg/mL
1h 30m
= 9.6 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Human PD-1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PD-1 in Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Alternative names=Programmed cell death protein 1, Protein PD-1, hPD-1, PD1, PDCD1
Colorimetric
Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
25 - 1600 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 9.6 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.4 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 9.6 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 93 | Range 90 - 96 % |
Sample type EDTA Plasma | Average % = 95 | Range 93 - 97 % |
Sample type Cell culture media | Average % = 95 | Range 93 - 97 % |
Sample type Tissue Extracts | Average % = 102 | Range 101 - 104 % |
Sample type Heparin Plasma | Average % = 92 | Range 90 - 94 % |
Sample type Citrate plasma | Average % = 94 | Range 93 - 96 % |
Sample type Cell Lysate | Average % = 98 | Range 95 - 100 % |
Blue Ice
+4°C
+4°C
Human PD-1 ELISA Kit (ab252360) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-1 protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PD-1 with 9.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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