Human PD-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PD-1 with a sensitivity of 9.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- Cancers 15:2023Association between Expression and Immune Dysregulation in Pancreatic Ductal Adenocarcinoma: Insights from Comprehensive Immune Profiling of Peripheral Blood Mononuclear Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArenida Bartkeviciene,Aldona Jasukaitiene,Inga Zievyte,Darius Stukas,Sandra Ivanauskiene,Daiva Urboniene,Toivo Maimets,Kristaps Jaudzems,Astra Vitkauskiene,Jason Matthews,Zilvinas Dambrauskas,Antanas GulbinasPubMed 37760608
- Cancer medicine 12:17648-176592023Serum soluble immune checkpoint levels predict cervical lymph node metastasis of differentiated thyroid carcinoma patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Shao,Xinru Gui,Yuxin Wang,Lei Sheng,Dong Sun,Qingdong Zeng,Huayang WangPubMed 37501393
- International journal of molecular sciences 24:2023Tumor-TME Bipartite Landscape of PD-1/PD-L1 in Endometrial Cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaed Sulaiman,Pradip De,Jennifer C Aske,Xiaoqian Lin,Adam Dale,Nischal Koirala,Kris Gaster,Luis Rojas Espaillat,David Starks,Nandini DeyPubMed 37446260
- Oncoimmunology 11:20963592022PD-1 is expressed in cytotoxic granules of NK cells and rapidly mobilized to the cell membrane following recognition of tumor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCecilia Pesini,Sandra Hidalgo,Maykel A Arias,Llipsy Santiago,Carlota Calvo,Maitane Ocariz-Díez,Dolores Isla,Pilar M Lanuza,M José Agustín,Eva M Galvez,Ariel Ramírez-Labrada,Julián PardoPubMed 35813574
- International journal of molecular sciences 23:2022Synthesis and Biological Evaluation of Small Molecules as Potential Anticancer Multitarget Agents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Pla-López,Raquel Castillo,Rocío Cejudo-Marín,Olaya García-Pedrero,Mariam Bakir-Laso,Eva Falomir,Miguel CardaPubMed 35806053
- Molecules (Basel, Switzerland) 27:2022Novel 1,2,3-Triazole-Coumarin Hybrid Glycosides and Their Tetrazolyl Analogues: Design, Anticancer Evaluation and Molecular Docking Targeting EGFR, VEGFR-2 and CDK-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWael A El-Sayed,Fahad M Alminderej,Marwa M Mounier,Eman S Nossier,Sayed M Saleh,Asmaa F KassemPubMed 35408446
- International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 115:229-2362021Circulating level of sPD-1 and PD-1 genetic variants are associated with hepatitis B infection and related liver disease progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPham Thi Minh Huyen,Dang Thi Ngoc Dung,Peter Johann Weiß,Phan Quoc Hoan,Dao Phuong Giang,Ngo Thi Uyen,Nguyen Van Tuan,Ngo Tat Trung,Thirumalaisamy P Velavan,Le Huu Song,Nghiem Xuan HoanPubMed 34910956
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 25 - 1600 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 9.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The protein expressed by the gene PDCD1 functions as an inhibitory receptor on antigen-activated T-cells, playing a critical role in the induction and maintenance of immune tolerance to self. It delivers inhibitory signals by binding to the ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Tumors exploit the PDCD1-mediated inhibitory pathway to attenuate anti-tumor immunity, enabling tumor survival by evading immune system destruction. The interaction with CD274/PDCD1L1 inhibits the effector function of cytotoxic T lymphocytes (CTLs). Blocking the PDCD1-mediated pathway can reverse the exhausted T-cell phenotype and normalize the anti-tumor response, providing a rationale for cancer immunotherapy. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1
What's included?
Recommended products
Human PD-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PD-1 with a sensitivity of 9.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 25 - 1600 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 9.6 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 2.4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 9.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 93 | Range 90 - 96 % |
Sample type EDTA Plasma | Average % = 95 | Range 93 - 97 % |
Sample type Cell culture media | Average % = 95 | Range 93 - 97 % |
Sample type Tissue Extracts | Average % = 102 | Range 101 - 104 % |
Sample type Heparin Plasma | Average % = 92 | Range 90 - 94 % |
Sample type Citrate plasma | Average % = 94 | Range 93 - 96 % |
Sample type Cell Lysate | Average % = 98 | Range 95 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human PD-1 ELISA Kit (ab252360) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-1 protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PD-1 with 9.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.
- Biological function summary
PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.
- Pathways
PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.
- Associated diseases and disorders
PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.
Product promise
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5 product images
Sandwich ELISA - Human PD-1 ELISA Kit (ab252360)
Example of human PD-1 standard curve in Sample Diluent NS.
The PD-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PD-1 ELISA Kit (ab252360)
Example of human PD-1 standard curve in 1X Cell Extraction Buffer PTR.
The PD-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PD-1 ELISA Kit (ab252360)
Interpolated concentrations of native PD-1 in human serum, plasma samples.
The concentrations of PD-1 were measured in duplicates, interpolated from the PD-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, plasma (EDTA) neat, plasma (heparin) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1 concentration was determined to be 119.1 pg/mL in serum, 88.1 pg/mL in plasma (citrate) and 85.9 pg/mL in plasma (EDTA), and 92.8 pg/mL in plasma (heparin).
Sandwich ELISA - Human PD-1 ELISA Kit (ab252360)
Interpolated concentrations of native PD-1 in human Molt-4 cell extract based on a 1000 ug/mL extract load and Thymus tissue extract samples based on a 500 μg/mL extract load.
The concentrations of PD-1 were measured in duplicate and interpolated from the PD-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1 concentration was determined to be 636.9 pg/mL in Molt-4 cell extract and 566.5 pg/mL in Thymus tissue extract.
Sandwich ELISA - Human PD-1 ELISA Kit (ab252360)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1 concentration was determined to be 86.56 pg/mL with a range of 47.82 – 210.99 pg/mL.
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