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AB326307

Human PD-L1 [28-8] ELISA Kit, Chemiluminescent

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Human PD-L1 [28-8] ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human PD-L1 [28-8] with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.19 - 4800 pg/ml with a sensitivity of 0.66pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1

7 Images
Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)
  • sELISA

Lab

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)
  • sELISA

Lab

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)

Interpolated concentration of native PD-L1 was measured in duplicate in human PBMC cell culture supernatants. Human PBMCs were cultured in the presence or absence of 1.5% PHA-M. The interpolated values (dilution factor correct to neat) are plotted (mean +/- SD, n=2). The mean concentration was determined to be 393 pg/mL in PHA-M stimulated PBMC supernatant and undetectable in untreated supernatant samples.

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)
  • sELISA

Lab

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)

Dynamic range comparison between the Chemiluminescent Human PD-L1 SimpleStep ELISA, colorimetric Human PD-L1 SimpleStep ELISA (ab277712), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)
  • sELISA

Lab

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)

Standard curve comparison between the Chemiluminescent Human PD-L1 SimpleStep ELISA and colorimetric Human PD-L1 SimpleStep ELISA (ab277712) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)
  • sELISA

Supplier Data

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)

Interpolated concentrations of native PD-L1 in human HDLM-2 cell extract based on a 10 µg/mL extract load.

The concentrations of PD-L1 were measured in duplicate and interpolated from the PD-L1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean PD-L1 concentration was determined to be 393.85 pg/mL in HDLM-2 extract.

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)
  • sELISA

Supplier Data

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)

Interpolated concentrations.

Undiluted samples are as follows : serum 95% and PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean PD-L1 concentration was determined to be 111.81 pg/mL in serum and 361.74 pg/mL in stimulated PBMC supernatant.

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)
  • sELISA

Unknown

Sandwich ELISA - Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (AB326307)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean PD-L1 concentration was determined to be 99.45 pg/mL with a range of 59.76 – 168.01 pg/mL.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Urine, Heparin Plasma, Citrate plasma, Cell culture extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.66 pg/mL

Range

2.19 - 4800 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (ab326307) is a rapid single-wash sandwich 55-min ELISA to measure Human PD-L1 [28-8] in Cell culture supernatant, Serum, EDTA Plasma, Urine, Heparin Plasma, Citrate plasma, Cell culture extracts with a sensitivity of 0.66 pg/mL.

How the assay works

Human PD-L1 [28-8] ELISA Kit, Chemiluminescent (ab326307) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human PD-L1 [28-8] (ab326307) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PD-L1 antibody [EPR23939-25] - BSA and Aside free (Capture) ab278009
- Anti-PD-L1 antibody [EPR5-28] - BSA and Aside free (Detector) ab278010
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.7" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 100 %", "average": "= 93" }, { "sample": "Serum", "range": "94 - 97 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "89 - 92 %", "average": "= 90" }, { "sample": "Urine", "range": "86 - 97 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "90 - 111 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "87 - 95 %", "average": "= 90" }, { "sample": "Cell culture extracts", "range": "99 - 106 %", "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed : 10581077). Can also act as a transcription coactivator : in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed : 32929201).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28813410, PubMed : 28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
See full target information CD274
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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