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AB277712

Human PD-L1 ELISA Kit [28-8]

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(15 Publications)

Human PD-L1 ELISA Kit [28-8] is a single-wash 90-min Simplestep used to quantify Human PD-L1 [28-8] with a sensitivity of 3.75 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1

9 Images
ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)
  • ELISA

Supplier Data

ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)
Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)
  • sELISA

Unknown

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

Example of human PD-L1 standard curve in Sample Diluent NS. The PD-L1 standard curve was prepared as described in Section 10.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)
  • sELISA

Supplier Data

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

Interpolated concentrations.

Undiluted samples are as follows : serum 95% and PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 111.81 pg/mL in serum and 361.74 pg/mL in stimulated PBMC supernatant.

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)
  • sELISA

Unknown

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 99.45 pg/mL with a range of 59.76 – 168.01 pg/mL.

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)
  • sELISA

Supplier Data

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

Interpolated concentrations of native PD-L1 in human HDLM-2 cell extract based on a 10 μg/mL extract load.

The concentrations of PD-L1 were measured in duplicate and interpolated from the PD-L1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 393.85 pg/mL in HDLM-2 extract.

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)
  • sELISA

Lab

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

Interpolated concentrations of human PD-L1 in HDLM-2 cell extract in 96-well vs. 384-well plates.

Interpolated concentration of native PD-L1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 10 µg/mL HDLM-2 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction PTR.

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)
  • sELISA

Lab

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

Example of human PD-L1 standard curve in 1X Cell Extraction PTR in 96-well vs. 384-well plate.

Example of human PD-L1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)
  • sELISA

Supplier Data

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

Example of human PD-L1 standard curve in 1X Cell Extraction Buffer PTR. The PD-L1 standard curve was prepared as described in Section 10.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)
  • sELISA

Lab

Sandwich ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

Example of human PD-L1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate.

Example of human PD-L1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.75 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PD-L1 ELISA Kit [28-8] ab277712 is a rapid single-wash 90-min Sandwich ELISA to measure Human PD-L1 [28-8]in cell culture supernatant, cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 3.75 pg/mL.

How the assay works

Human PD-L1 [28-8]SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human PD-L1 ELISA Kit [28-8] ab277712 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human PD-L1 ELISA Kit [28-8] only requires a maximum of 12.5µL sample

PD-L1 (also known as CD274 or B7-H1) is a membrane bound glycoprotein involved in regulation of the immune system. PD-L1 is expressed on a variety of inflammatory-activated cells as well as some carcinomas and in melanoma. PD-L1 binds to PD-1 and CD80, where it can suppress T cell activation and proliferation as well as induce apoptosis. Levels of PD-L1 are increased in the plasma of cancer patients as well as in cerebrospinal fluid of gliomas. PD-L1 can bind PD-1 in order to regulate T cell apoptosis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 100 %", "average": "= 93" }, { "sample": "Serum", "range": "94 - 97 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "89 - 92 %", "average": "= 90" }, { "sample": "Urine", "range": "86 - 97 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "90 - 111 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "87 - 95 %", "average": "= 90" }, { "sample": "Cell culture extracts", "range": "99 - 106 %", "average": "= 102" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.
Biological function summary

PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.

Pathways

PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.

PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.
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Product protocols

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Target data

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed : 10581077). Can also act as a transcription coactivator : in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed : 32929201).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28813410, PubMed : 28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
See full target information CD274
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 15:1555307 PubMed40568710

2025

Assessment of S100A8/A9 and resistin as predictive biomarkers for mortality in critically ill patients with sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Zhengquan Liu,Fan Zhou,Ye Sun,Zhenyou Jiang,Pingsen Zhao

Frontiers in medicine 12:1530804 PubMed39958825

2025

Increased serum soluble PD-l1 levels in patients with advanced stages of chronic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ayaka Hayashi,Hiroto Ishihara,Mayuko Kawabe,Kazuhiko Kato,Akio Nakashima,Izumi Yamamoto,Teppei Sakano,Hiroe Kobashi,Makoto Morita,Takashi Yokoo,Mitsuyoshi Urashima

Nature communications 16:1295 PubMed39900923

2025

Plant-nanoparticles enhance anti-PD-L1 efficacy by shaping human commensal microbiota metabolites.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Teng,Chao Luo,Xiaolan Qiu,Jingyao Mu,Mukesh K Sriwastva,Qingbo Xu,Minmin Liu,Xin Hu,Fangyi Xu,Lifeng Zhang,Juw Won Park,Jae Yeon Hwang,Maiying Kong,Zhanxu Liu,Xiang Zhang,Raobo Xu,Jun Yan,Michael L Merchant,Craig J McClain,Huang-Ge Zhang

Journal for immunotherapy of cancer 12: PubMed39357979

2024

Gal-3 blocks the binding between PD-1 and pembrolizumab.

Applications

Unspecified application

Species

Unspecified reactive species

Stinne Ravn Greisen,Mia Bendix,Morten Aagaard Nielsen,Kathrine Pedersen,Nina Haunstrup Jensen,Malene Hvid,Jakob Hauge Mikkelsen,Taner Drace,Thomas Boesen,Torben Steiniche,Henrik Schmidt,Bent Deleuran

Frontiers in immunology 15:1365894 PubMed38779680

2024

Plasminogen activator inhibitor-1 promotes immune evasion in tumors by facilitating the expression of programmed cell death-ligand 1.

Applications

Unspecified application

Species

Unspecified reactive species

Abd Aziz Ibrahim,Taku Fujimura,Tomoko Uno,Tomoya Terada,Ken-Ichi Hirano,Hiroyuki Hosokawa,Akio Ohta,Toshio Miyata,Kiyoshi Ando,Takashi Yahata

Journal of experimental & clinical cancer research : CR 43:115 PubMed38627816

2024

CCAAT enhancer binding protein delta activates vesicle associated membrane protein 3 transcription to enhance chemoresistance and extracellular PD-L1 expression in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhao,Yangyang Yu,Xiangmin Li,Ayao Guo

The Journal of dermatology 51:807-815 PubMed38433350

2024

Soluble PD-L1 predicts tumor response and immune-related adverse events in patients with advanced melanoma treated with anti-PD-1 antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Kazumasa Oya,Yoshiyuki Nakamura,Larina Tzu-Wei Shen,Shoichiro Ishizuki,Satoshi Matsusaka,Yasuhiro Fujisawa

Cells 12: PubMed38132164

2023

Trans-Regulation of Alternative mRNA Processing by CDK12 in Non-Small-Cell Lung Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Trine V Larsen,Christoffer T Maansson,Tina F Daugaard,Brage S Andresen,Boe S Sorensen,Anders L Nielsen

Medicine 102:e35757 PubMed37933048

2023

Expression of PD-1/PD-L1 in peripheral blood and tumor tissues of patients with classical Hodgkin's lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Feng,Xiangyi Luo,Yingmei Yang,Yuchen Fan,Qing Ye

Cancers 15: PubMed37760608

2023

Association between Expression and Immune Dysregulation in Pancreatic Ductal Adenocarcinoma: Insights from Comprehensive Immune Profiling of Peripheral Blood Mononuclear Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Arenida Bartkeviciene,Aldona Jasukaitiene,Inga Zievyte,Darius Stukas,Sandra Ivanauskiene,Daiva Urboniene,Toivo Maimets,Kristaps Jaudzems,Astra Vitkauskiene,Jason Matthews,Zilvinas Dambrauskas,Antanas Gulbinas
View all publications
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