AB231928

Human PD-L2 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Human PD-L2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PD-L2 with a sensitivity of 2.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD273, B7DC, PDCD1L2, PDL2, PDCD1LG2, Programmed cell death 1 ligand 2, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC

6 Images

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

sELISA

Supplier Data

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Interpolated concentrations of native PD-L2 in human PBMC cell culture supernatant samples stimulated or unstimulated with PHA/PMA.

The concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 32 pg/mL in stimulated PBMC cell culture supernatant and undetectable in unstimulated and media controls.

sELISA

Supplier Data

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Interpolated concentrations of native PD-L2 in human urine and milk samples.

The concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 50% and milk 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 2172 pg/mL in urine and 151 pg/mL in milk.

sELISA

Supplier Data

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 10.3 ng/mL with a range of 7.5 – 15.0 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Interpolated concentrations of native PD-L2 in human serum and plasma samples.

The concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 10%, plasma (EDTA) 5%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 9.7 ng/mL in serum, 11.1 ng/mL in plasma (citrate), 7.6 ng/mL in plasma (EDTA) and 8.5 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Interpolated concentrations of spiked PD-L2 in human saliva and cell culture media samples.

The concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Example of human PD-L2 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.7 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human PD-L2 ELISA Kit (ab231928) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-L2 protein in edta plasma, hep plasma, milk, saliva, serum, urine, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PD-L2 with 2.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]

style

left-column

Recovery

[ { "sample": "Milk", "range": "77 - 85 %", "average": "= 82" }, { "sample": "Serum", "range": "101 - 104 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "89 - 96 %", "average": "= 92" }, { "sample": "Saliva", "range": "101 - 103 %", "average": "= 102" }, { "sample": "Urine", "range": "92 - 100 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "95 - 107 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "92 - 106 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "99 - 99 %", "average": "= 99" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab231928-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human PD-L2 Detector Antibody", "number":"AB231928-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB231928-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB231928-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB231928-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB231928-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB231928-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB231928-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB231928-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PD-L2 Capture Antibody", "number":"AB231928-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PD-L2 Lyophilized Recombinant Protein", "number":"AB231928-CMP03", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD-L2 also known as PDCD1LG2 or B7-DC is a member of the B7 protein family that plays a role in immune system regulation. This protein has a molecular mass of approximately 25 kDa. PD-L2 is expressed in antigen-presenting cells such as dendritic cells and macrophages. Expression is also located in some subsets of T cells and B cells. The PD-L2 can act as a second ligand for the PD-1 receptor which is important in immune checkpoints.

Biological function summary

PD-L2 offers protection to tissues from autoimmunity by regulating T-cell activation and fostering tolerance. By engaging with its receptor PD-1 PD-L2 forms a complex that modulates the immune response to prevent overactivation. This interaction limits the immune system's potential damage to healthy tissues maintaining proper immune function in diverse environments.

Pathways

PD-L2 participates in the immune checkpoint pathways where it works closely with PD-1 to mediate immune responses. PD-L2 forms an important part of the program death-1 (PD-1) pathway which plays a prominent role in downregulating T-cell activity. It relates to proteins such as PD-L1 which also interacts with PD-1 modulating immune tolerance and response to infections or tumors.

PD-L2 involvement has been significant in cancer immunology and autoimmune conditions. Its interaction with PD-1 relates to the suppression of immune responses in tumors contributing to cancer progression. PD-L2's function as an immune checkpoint ligand associates with autoimmune diseases like rheumatoid arthritis where immune regulation goes awry. Both PD-1 and PD-L2 represent potential targets for therapeutic interventions due to their roles in these conditions.

style

left-column

Product protocols

style

left-column

Target data

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11224527, PubMed : 12538684). Acts as a ligand for the inhibitory receptor PDCD1/PD-1, inhibiting T-cell proliferation by blocking cell cycle progression and cytokine production (PubMed : 11224527).

See full target information PDCD1LG2

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Current oncology (Toronto, Ont.) 31:1701-1712 PubMed38668032

2024

Soluble Immune Checkpoint Molecules as Predictors of Efficacy in Immuno-Oncology Combination Therapy in Advanced Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kosuke Ueda,Keiichiro Uemura,Naoki Ito,Yuya Sakai,Satoshi Ohnishi,Hiroki Suekane,Hirofumi Kurose,Tasuku Hiroshige,Katsuaki Chikui,Kiyoaki Nishihara,Makoto Nakiri,Shigetaka Suekane,Sachiko Ogasawara,Hirohisa Yano,Tsukasa Igawa

International journal of molecular sciences 24: PubMed37446260

2023

Tumor-TME Bipartite Landscape of PD-1/PD-L1 in Endometrial Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Raed Sulaiman,Pradip De,Jennifer C Aske,Xiaoqian Lin,Adam Dale,Nischal Koirala,Kris Gaster,Luis Rojas Espaillat,David Starks,Nandini Dey

American journal of reproductive immunology (New York, N.Y. : 1989) 89:e13649 PubMed36394352

2022

Expression and T cell regulatory action of the PD-1 immune checkpoint in the ovary and fallopian tube.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua Johnson,So-Youn Kim,Peter Ka Sam,Rengasamy Asokan,Evelyn Llerena Cari,Elise S Bales,Thanh-Ha Luu,Lauren Perez,Amanda N Kallen,Liesl Nel-Themaat,Alex J Polotsky,Miriam D Post,David J Orlicky,Kimberly R Jordan,Benjamin G Bitler

Shock (Augusta, Ga.) 52:13-22 PubMed30036273

2018

Integrin and PD-1 Ligand Expression on Circulating Extracellular Vesicles in Systemic Inflammatory Response Syndrome and Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Eiji Kawamoto,Asami Masui-Ito,Akiko Eguchi,Zay Yar Soe,Onmanee Prajuabjinda,Samuel Darkwah,Eun Jeong Park,Hiroshi Imai,Motomu Shimaoka

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PD-L2 ELISA Kit

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Sandwich ELISA - Human PD-L2 ELISA Kit (AB231928)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Secondary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise