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AB100622

Human PDGF AA ELISA Kit

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(4 Publications)

Human PDGF AA ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PDGF AA in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD306, LAIR2, Leukocyte-associated immunoglobulin-like receptor 2, LAIR-2

1 Images
Sandwich ELISA - Human PDGF AA ELISA Kit (AB100622)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF AA ELISA Kit (AB100622)

Representative Standard Curve using ab100622.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 40 pg/mL

Range

40 - 30000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's PDGF AA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AA in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human PDGF AA coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 102 %", "average": "= 92.54" }, { "sample": "Serum", "range": "70 - 83 %", "average": "= 76.71" }, { "sample": "Plasma", "range": "72 - 85 %", "average": "= 78.06" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100622-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100622-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100622-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100622-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human PDGF AA Standard (lyophilized)", "number":"AB100622-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100622-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human PDGF AA", "number":"AB100622-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100622-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PDGF AA Microplate (12 x 8 wells)", "number":"AB100622-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Platelet-derived growth factor AA or PDGF-AA is a member of the PDGF protein family which is involved in various cellular processes. The PDGF-AA protein has a molecular mass of approximately 24 kDa. It is expressed in a wide range of tissues including fibroblasts smooth muscle cells and neurons. The protein is considered a homodimer composed of two A chains and it plays an important role in cell signaling by binding to PDGF receptors on the surface of target cells.
Biological function summary

PDGF-AA influences cell proliferation migration and differentiation. It acts as a mitogen especially for mesenchymal cells such as fibroblasts and smooth muscle cells. PDGF-AA is often part of a signaling complex where it activates PDGF receptor-alpha (PDGFR-α) leading to autophosphorylation and subsequent activation of downstream effectors. This strengthens tissue development and wound healing processes.

Pathways

PDGF-AA integrates into critical signaling pathways like the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell growth and survival. PDGF-AA binds specifically to PDGFR-α which shares importance with other PDGF isoforms such as PDGF-BB in orchestrating cellular responses through similar pathways thereby ensuring precise physiological outcomes.

PDGF-AA is implicated in conditions like cancer and fibrosis. Aberrant signaling involving PDGF-AA can lead to unchecked cell proliferation contributing to tumorigenesis. Similarly PDGF-AA is connected to fibrotic diseases where excessive tissue scarring occurs due to overactive fibroblast stimulation. PDGF-BB another member of the PDGF protein family often gets studied alongside PDGF-AA for their combined roles in these pathological states.
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Product protocols

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Target data

See full target information LAIR2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37175778

2023

Intraocular Pressure-Lowering and Retina-Protective Effects of Exosome-Rich Conditioned Media from Human Amniotic Membrane Stem Cells in a Rat Model of Glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Rim Seong,Chan Ho Noh,Sangryong Park,Sumin Cho,Seok-Jin Hong,Ah-Young Lee,Dongho Geum,Soon-Cheol Hong,Dongsun Park,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae Kim

Endocrine and metabolic science 3: PubMed35935682

2021

Elevated neuregulin-1 levels correlate with plasma biomarkers of cerebral injury and high stroke risk in children with sickle cell anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Chambliss,Tatayana Richardson,John Onyekaba,Juan Cespedes,Annette Nti,Keri Oxendine Harp,Iris Buchanan-Perry,Jonathan K Stiles,Beatrice E Gee

Journal of inflammation research 12:335-343 PubMed31908518

2019

Modulation of Human Neutrophil Peptides on Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles.

Applications

Unspecified application

Species

Unspecified reactive species

Qingqing Dai,Yasumasa Morita,Yongbo Huang,Patricia C Liaw,Jianfeng Wu,Julie Khang,Diana Islam,Kaijiang Yu,Yimin Li,Haibo Zhang

Scientific reports 8:5805 PubMed29643433

2018

Neurofibromin knockdown in glioma cell lines is associated with changes in cytokine and chemokine secretion in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew D Wood,Joydeep Mukherjee,Russell O Pieper
View all publications
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