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Human PDGF AA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PDGF AA in Cell culture media, Citrate plasma, Serum, EDTA Plasma samples.

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Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

140.625 - 9000 pg/mL

Assay time

1h 30m

Sensitivity

= 18.6 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human PDGF AA Capture Antibody
1 x 600 µL
10X Human PDGF AA Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CPI2
1 x 6 mL
Human PDGF AA Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent 50BP
1 x 20 mL
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Human PDGF AA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PDGF AA in Cell culture media, Citrate plasma, Serum, EDTA Plasma samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

140.625 - 9000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 18.6 pg/mL

Precision

Intra assay

Sample

Supernatant

n

8

mean

-

SD

-

C.V.

3.6

Inter assay

Sample

Supernatant

n

3

mean

-

SD

-

C.V.

2.2

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 121

Range

110 - 126 %

Sample type

EDTA Plasma

Average %

= 81

Range

78 - 84 %

Sample type

Cell culture media

Average %

= 114

Range

111 - 117 %

Sample type

Citrate plasma

Average %

= 103

Range

96 - 110 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human PDGF AA ELISA kit (ab284616) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PDGF AA protein in human serum, plasma, cell culture media samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PDGF AA with 18.6 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Supplementary info

Biological function summary

PDGF-AA influences cell proliferation migration and differentiation. It acts as a mitogen especially for mesenchymal cells such as fibroblasts and smooth muscle cells. PDGF-AA is often part of a signaling complex where it activates PDGF receptor-alpha (PDGFR-α) leading to autophosphorylation and subsequent activation of downstream effectors. This strengthens tissue development and wound healing processes.

Activity summary

Platelet-derived growth factor AA or PDGF-AA is a member of the PDGF protein family which is involved in various cellular processes. The PDGF-AA protein has a molecular mass of approximately 24 kDa. It is expressed in a wide range of tissues including fibroblasts smooth muscle cells and neurons. The protein is considered a homodimer composed of two A chains and it plays an important role in cell signaling by binding to PDGF receptors on the surface of target cells.

Pathways

PDGF-AA integrates into critical signaling pathways like the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell growth and survival. PDGF-AA binds specifically to PDGFR-α which shares importance with other PDGF isoforms such as PDGF-BB in orchestrating cellular responses through similar pathways thereby ensuring precise physiological outcomes.

Associated diseases and disorders

PDGF-AA is implicated in conditions like cancer and fibrosis. Aberrant signaling involving PDGF-AA can lead to unchecked cell proliferation contributing to tumorigenesis. Similarly PDGF-AA is connected to fibrotic diseases where excessive tissue scarring occurs due to overactive fibroblast stimulation. PDGF-BB another member of the PDGF protein family often gets studied alongside PDGF-AA for their combined roles in these pathological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616), expandable thumbnail

    Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616)

    Example of human PDGF AA standard curve in Sample Diluent 50BP.

    Example of human PDGF AA standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616), expandable thumbnail

    Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616)

    Interpolated concentrations of human PDGF AA in serum, plasma (citrate) and plasma (EDTA).

    Interpolated concentration of native PDGF AA was measured in duplicate. Undiluted samples are 100% serum (pooled), 25% serum (individual), plasma (citrate), plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 50BP.

  • Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616), expandable thumbnail

    Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616)

    Interpolated concentrations of spiked human PDGF AA in cell culture media.

    Recombinant PDGF AA was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent 50BP. Undiluted sample is 100% 10F RPMI 1640 cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616), expandable thumbnail

    Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616)

    Interpolated concentrations of PDGF AA in normal human serum donors.

    Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean PDGF AA concentration was determined to be 6,238 pg/mL with a range of 281 – 24532 pg/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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