Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Human PDGF AA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PDGF AA in Cell culture media, Citrate plasma, Serum, EDTA Plasma samples.
Colorimetric
Cell culture media, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
140.625 - 9000 pg/mL
1h 30m
= 18.6 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).
Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGFA, PDGF1
Human PDGF AA ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PDGF AA in Cell culture media, Citrate plasma, Serum, EDTA Plasma samples.
Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGFA, PDGF1
Colorimetric
Cell culture media, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
140.625 - 9000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 18.6 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 3.6 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 2.2 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 121 | Range 110 - 126 % |
Sample type EDTA Plasma | Average % = 81 | Range 78 - 84 % |
Sample type Cell culture media | Average % = 114 | Range 111 - 117 % |
Sample type Citrate plasma | Average % = 103 | Range 96 - 110 % |
Blue Ice
+4°C
+4°C
Human PDGF AA ELISA kit (ab284616) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PDGF AA protein in human serum, plasma, cell culture media samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PDGF AA with 18.6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
PDGF-AA influences cell proliferation migration and differentiation. It acts as a mitogen especially for mesenchymal cells such as fibroblasts and smooth muscle cells. PDGF-AA is often part of a signaling complex where it activates PDGF receptor-alpha (PDGFR-α) leading to autophosphorylation and subsequent activation of downstream effectors. This strengthens tissue development and wound healing processes.
Platelet-derived growth factor AA or PDGF-AA is a member of the PDGF protein family which is involved in various cellular processes. The PDGF-AA protein has a molecular mass of approximately 24 kDa. It is expressed in a wide range of tissues including fibroblasts smooth muscle cells and neurons. The protein is considered a homodimer composed of two A chains and it plays an important role in cell signaling by binding to PDGF receptors on the surface of target cells.
PDGF-AA integrates into critical signaling pathways like the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell growth and survival. PDGF-AA binds specifically to PDGFR-α which shares importance with other PDGF isoforms such as PDGF-BB in orchestrating cellular responses through similar pathways thereby ensuring precise physiological outcomes.
PDGF-AA is implicated in conditions like cancer and fibrosis. Aberrant signaling involving PDGF-AA can lead to unchecked cell proliferation contributing to tumorigenesis. Similarly PDGF-AA is connected to fibrotic diseases where excessive tissue scarring occurs due to overactive fibroblast stimulation. PDGF-BB another member of the PDGF protein family often gets studied alongside PDGF-AA for their combined roles in these pathological states.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Example of human PDGF AA standard curve in Sample Diluent 50BP.
Example of human PDGF AA standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of human PDGF AA in serum, plasma (citrate) and plasma (EDTA).
Interpolated concentration of native PDGF AA was measured in duplicate. Undiluted samples are 100% serum (pooled), 25% serum (individual), plasma (citrate), plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 50BP.
Interpolated concentrations of spiked human PDGF AA in cell culture media.
Recombinant PDGF AA was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent 50BP. Undiluted sample is 100% 10F RPMI 1640 cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Interpolated concentrations of PDGF AA in normal human serum donors.
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean PDGF AA concentration was determined to be 6,238 pg/mL with a range of 281 – 24532 pg/mL.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com