Human PDGF AA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PDGF AA with a sensitivity of 18.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 140.625 - 9000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 18.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).
Alternative names
PDGF1, PDGFA, Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide
What's included?
Recommended products
Human PDGF AA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PDGF AA with a sensitivity of 18.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 140.625 - 9000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 18.6 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 8 | C.V. 3.6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 3 | C.V. 2.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 121 | Range 110 - 126 % |
Sample type EDTA Plasma | Average % = 81 | Range 78 - 84 % |
Sample type Cell culture media | Average % = 114 | Range 111 - 117 % |
Sample type Citrate plasma | Average % = 103 | Range 96 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human PDGF AA ELISA kit (ab284616) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PDGF AA protein in human serum, plasma, cell culture media samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PDGF AA with 18.6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Platelet-derived growth factor AA or PDGF-AA is a member of the PDGF protein family which is involved in various cellular processes. The PDGF-AA protein has a molecular mass of approximately 24 kDa. It is expressed in a wide range of tissues including fibroblasts smooth muscle cells and neurons. The protein is considered a homodimer composed of two A chains and it plays an important role in cell signaling by binding to PDGF receptors on the surface of target cells.
- Biological function summary
PDGF-AA influences cell proliferation migration and differentiation. It acts as a mitogen especially for mesenchymal cells such as fibroblasts and smooth muscle cells. PDGF-AA is often part of a signaling complex where it activates PDGF receptor-alpha (PDGFR-α) leading to autophosphorylation and subsequent activation of downstream effectors. This strengthens tissue development and wound healing processes.
- Pathways
PDGF-AA integrates into critical signaling pathways like the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell growth and survival. PDGF-AA binds specifically to PDGFR-α which shares importance with other PDGF isoforms such as PDGF-BB in orchestrating cellular responses through similar pathways thereby ensuring precise physiological outcomes.
- Associated diseases and disorders
PDGF-AA is implicated in conditions like cancer and fibrosis. Aberrant signaling involving PDGF-AA can lead to unchecked cell proliferation contributing to tumorigenesis. Similarly PDGF-AA is connected to fibrotic diseases where excessive tissue scarring occurs due to overactive fibroblast stimulation. PDGF-BB another member of the PDGF protein family often gets studied alongside PDGF-AA for their combined roles in these pathological states.
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4 product images
Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616)
Example of human PDGF AA standard curve in Sample Diluent 50BP.
Example of human PDGF AA standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616)
Interpolated concentrations of human PDGF AA in serum, plasma (citrate) and plasma (EDTA).
Interpolated concentration of native PDGF AA was measured in duplicate. Undiluted samples are 100% serum (pooled), 25% serum (individual), plasma (citrate), plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 50BP.
Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616)
Interpolated concentrations of spiked human PDGF AA in cell culture media.
Recombinant PDGF AA was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent 50BP. Undiluted sample is 100% 10F RPMI 1640 cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human PDGF AA ELISA Kit (ab284616)
Interpolated concentrations of PDGF AA in normal human serum donors.
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean PDGF AA concentration was determined to be 6,238 pg/mL with a range of 281 – 24532 pg/mL.
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