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AB100623

Human PDGF AB ELISA Kit

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(1 Publication)

Human PDGF AB ELISA Kit is a Sandwich ELISA for the measurement of Human PDGF AB in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

2 Images
Sandwich ELISA - Human PDGF AB ELISA Kit (AB100623)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF AB ELISA Kit (AB100623)

Representative Standard Curve using ab100623.

Sandwich ELISA - Human PDGF AB ELISA Kit (AB100623)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF AB ELISA Kit (AB100623)

Representative Standard Curve using ab100623.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

10.24 - 2500 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's PDGF AB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AB in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human PDGF AB coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "68 - 78 %", "average": "= 74.29" }, { "sample": "Serum", "range": "67 - 78 %", "average": "= 73.96" }, { "sample": "Plasma", "range": "67 - 79 %", "average": "= 74.85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100623-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100623-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100623-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human PDGF AB", "number":"AB100623-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PDGF AB Microplate (12 x 8 wells)", "number":"AB100623-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100623-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100623-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human PDGF AB Standard (lyophilized)", "number":"AB100623-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100623-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100623-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGF AB also known as Platelet-Derived Growth Factor AB is a heterodimeric protein with an approximate mass around 30 kDa. The PDGF AB protein plays an important role in cell proliferation and differentiation by activating its receptor PDGFR. It gets expressed in a variety of cell types including fibroblasts smooth muscle cells and endothelial cells. This growth factor influences cellular behaviors through its interactions with cells in connective tissues where it shows significant levels of expression.
Biological function summary

The PDGF AB protein promotes cellular proliferation and migration particularly in the context of wound healing and development. It forms part of a larger family of PDGF molecules that include homodimers and heterodimers and it requires specific receptor binding to exert its function. As a member of the growth factor family PDGF AB contributes to the signaling pathway that regulates tissue growth and repair processes.

Pathways

PDGF AB fits significantly into the PI3K/AKT and MAPK signaling pathways. These pathways are key in regulating cellular growth and survival and they involve interactions with important proteins like Ras and AKT. The activity of PDGF AB in these pathways affects various downstream molecular processes making it an integral component of cellular signaling networks.

PDGF AB connects to a few pathologies notably atherosclerosis and certain forms of cancer. It often associates with diseases where aberrant cell proliferation and migration occur. For instance in atherosclerosis PDGF AB interacts with proteins such as Integrins and MMPs which contribute to the remodeling of blood vessels. Similarly in oncology the dysregulation of PDGF AB can lead to malignancies with its interaction through growth-promoting pathways.
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Product protocols

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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed : 26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
See full target information PDGFB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 13: PubMed37763186

2023

Factors Affecting the Potential Efficacy of Intrauterine Platelet-Rich Plasma Infusion on Thin Endometrium in Women with Recurrent Implantation Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Pin-Yao Lin,Chun-I Lee,Yi-Chun Chen,En-Hui Cheng,Chun-Chia Huang,Chung-I Chen,Tsung-Hsien Lee,Yu-Jen Lee,Maw-Sheng Lee
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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