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AB300321

Human PDGF B ELISA Kit

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Human PDGF B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PDGF B with a sensitivity of 2.357 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

7 Images
Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)

Interpolated concentrations of human PDGF B in serum, plasma (EDTA), plasma (citrate), and plasma (heparin).

Interpolated concentration of native PDGF B was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 12.5%, plasma (EDTA) 12.5%, plasma (citrate) 25%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)

PDGFB monomer and homodimer reactivity.

Recombinant human PDGF B monomeric form and recombinant human PDGF B dimeric form were measured in duplicates at 250 pg/mL. Interpolated values are plotted (mean +- SD, n=2).

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)

Example of human PDGF B standard curve in Sample Diluent NS.

Example of human PDGF B standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)

Interpolated concentrations of PDGF B in normal human serum donors.

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean PDGF B concentration of detected samples was determined to be 1607.65 pg/mL with a range of undetected – 2896.75 pg/mL.

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)

Interpolated concentrations of human PDGF B in stimulated PBMC culture supernatant.

Interpolated concentration of native PDGF B was measured in duplicate at different sample concentrations. Undiluted samples are 50% stimulated (1.5% PHA-M, 2-days) PBMC cell culture (107 cells/mL) supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)

Standard curve comparison between the original Human PDGF B SimpleStep ELISA (ab184860) and current Human PDGF B SimpleStep ELISA (ab300321).

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF B ELISA Kit (AB300321)

Standard curve comparison between Human PDGF B SimpleStep ELISA kit (ab300321) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.357 pg/mL

Range

7.813 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "80 - 122 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "81 - 89 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "110 - 124 %", "average": "= 118" }, { "sample": "Heparin Plasma", "range": "93 - 116 %", "average": "= 105" }, { "sample": "Serum", "range": "95 - 103 %", "average": "= 99" } ]
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What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab300321-96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB300321-CMP18", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB300321-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB300321-CMP17", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PDGF B Lyophilized Recombinant Protein", "number":"AB300321-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB300321-CMP16", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB300321-CMP14", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PDGF B Capture Antibody", "number":"AB300321-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB300321-CMP20", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB300321-CMP19", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PDGF B Detector Antibody", "number":"AB300321-CMP12", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
Biological function summary

PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor β (PDGFR-β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.

Pathways

PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.

Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.
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Product protocols

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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed : 26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
See full target information PDGFB
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websiteProtocolBooklet
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