Human PDGF B ELISA Kit, Fluorescent (ab300326)

Human PDGF B ELISA Kit, Fluorescent is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PDGF B , Fluorescent in Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.

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Alternative names=Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, PDGFB, SIS, PDGF2

Detection method: Fluorescent
Sample types: Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 1.95 - 4000 pg/mL
Assay time: 1h 30m
Sensitivity: = 1.57 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Images

Target data

PDGFB

What's included?

96 Tests

Components

10X Human PDGF B, Fluorescent Capture Antibody

1 x 600 µL

10X Human PDGF B, Fluorescent Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

Antibody Diluent 5BI

1 x 6 mL

Human PDGF B, Fluorescent Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

1 x 12 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

1 x 1 Unit

Stop Solution

1 x 12 mL

TMB Development Solution

1 x 12 mL

Key facts

Detection method: Fluorescent
Sample types: Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 1.95 - 4000 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 1.57 pg/mL

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Plasma	n 8	mean -	SD -	C.V. 4.7

Inter assay

Sample	n	mean	SD	C.V.
Sample Plasma	n 3	mean -	SD -	C.V. 6

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 108	Range 80 - 122 %
Sample type Citrate plasma	Average % = 86	Range 81 - 89 %
Sample type EDTA Plasma	Average % = 118	Range 110 - 124 %
Sample type Heparin Plasma	Average % = 105	Range 93 - 116 %
Sample type Serum	Average % = 99	Range 95 - 103 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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Human PDGF B ELISA Kit, Fluorescent

Reactivity data

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