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AB100624

Human PDGF BB ELISA Kit

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(10 Publications)

Human PDGF BB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PDGF BB in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

2 Images
Sandwich ELISA - Human PDGF BB ELISA Kit (AB100624)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF BB ELISA Kit (AB100624)

Representative Standard Curve using ab100624.

Sandwich ELISA - Human PDGF BB ELISA Kit (AB100624)
  • sELISA

Supplier Data

Sandwich ELISA - Human PDGF BB ELISA Kit (AB100624)

Representative Standard Curve using ab100624.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 1 pg/mL

Range

0.549 - 400 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's PDGF BB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF BB in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human PDGF BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human PDGF BB ELISA Kit (ab184860) from our SimpleStep ELISA® range.

Note: Optimization may be required with urine samples, and very low level are detectable in serum

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 104 %", "average": "= 95.43" }, { "sample": "Serum", "range": "82 - 103 %", "average": "= 93.57" }, { "sample": "Plasma", "range": "83 - 104 %", "average": "= 91.99" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100624-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100624-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100624-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100624-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human PDGF BB Standard (lyophilized)", "number":"AB100624-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100624-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PDGF BB Microplate (12 x 8 wells)", "number":"AB100624-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB100624-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100624-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human PDGF BB", "number":"AB100624-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
Biological function summary

PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor β (PDGFR-β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.

Pathways

PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.

Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.
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Product protocols

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Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed : 26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
See full target information PDGFB
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:12414 PubMed40216867

2025

Liver quad culture chip as a model for radiation injury research.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Ueda,Satoshi Omiya,Jonathan Pinney,Michelle A Bylicky,Molykutty J Aryankalayil

Nature communications 12:3352 PubMed34099640

2021

The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets.

Applications

Unspecified application

Species

Unspecified reactive species

Henry Nording,Lasse Baron,David Haberthür,Frederic Emschermann,Matthias Mezger,Manuela Sauter,Reinhard Sauter,Johannes Patzelt,Kai Knoepp,Anne Nording,Moritz Meusel,Roza Meyer-Saraei,Ruslan Hlushchuk,Daniel Sedding,Oliver Borst,Ingo Eitel,Christian M Karsten,Robert Feil,Bernd Pichler,Jeanette Erdmann,Admar Verschoor,Emmanouil Chavakis,Triantafyllos Chavakis,Philipp von Hundelshausen,Jörg Köhl,Meinrad Gawaz,Harald F Langer

Scientific reports 9:8028 PubMed31142771

2019

Interplay of fibroblasts with anaplastic tumor cells promotes follicular thyroid cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Fozzatti,Vanina Alejandra Alamino,Sunmi Park,Lucila Giusiano,Ximena Volpini,Li Zhao,Cinthia Carolina Stempin,Ana Carolina Donadio,Sheue-Yann Cheng,Claudia Gabriela Pellizas

International journal of molecular sciences 20: PubMed30634448

2019

Establishing Liposome-Immobilized Dexamethasone-Releasing PDMS Membrane for the Cultivation of Retinal Pigment Epithelial Cells and Suppression of Neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Tzu-Wei Lin,Yueh Chien,Yi-Ying Lin,Mong-Lien Wang,Aliaksandr A Yarmishyn,Yi-Ping Yang,De-Kuang Hwang,Chi-Hsien Peng,Chih-Chien Hsu,Shih-Jen Chen,Ke-Hung Chien

Biomedicine & pharmacotherapy = Biomedecine & phar 107:359-367 PubMed30099339

2018

MiR-148b-3p inhibits renal carcinoma cell growth and pro-angiogenic phenotype of endothelial cell potentially by modulating FGF2.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Qing Ye,Zhenfang Du,Min Huang,Ming Zhang,Huifeng Tan

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association 34:581-591 PubMed29100775

2017

Adipose Stem Cells Incorporated in Fibrin Clot Modulate Expression of Growth Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Kelsy R Siegel,Tracy N Clevenger,Dennis O Clegg,Duncan A Proctor,Christopher S Proctor

Biochemistry and biophysics reports 11:154-160 PubMed28955780

2017

Pharmacological treatment with diacerein combined with mechanical stimulation affects the expression of growth factors in human chondrocytes.

Applications

sELISA

Species

Human

Bibiane Steinecker-Frohnwieser,Heike Kaltenegger,Lukas Weigl,Anda Mann,Werner Kullich,Andreas Leithner,Birgit Lohberger

Carcinogenesis 36:894-903 PubMed26026166

2015

miR-27 is associated with chemoresistance in esophageal cancer through transformation of normal fibroblasts to cancer-associated fibroblasts.

Applications

ELISA

Species

Human

Koji Tanaka,Hiroshi Miyata,Keijiro Sugimura,Shuichi Fukuda,Takashi Kanemura,Kotaro Yamashita,Yasuhiro Miyazaki,Tsuyoshi Takahashi,Yukinori Kurokawa,Makoto Yamasaki,Hisashi Wada,Kiyokazu Nakajima,Shuji Takiguchi,Masaki Mori,Yuichiro Doki

Frontiers in aging neuroscience 6:191 PubMed25136316

2014

The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress.

Applications

sELISA

Species

Human

Yun-Ching Chang,Wei-Chao Chang,Kuo-Hsuan Hung,Der-Ming Yang,Yung-Hsin Cheng,Yi-Wen Liao,Lin-Chung Woung,Ching-Yao Tsai,Chih-Chien Hsu,Tai-Chi Lin,Jorn-Hon Liu,Shih-Hwa Chiou,Chi-Hsien Peng,Shih-Jen Chen

Scientific reports 4:4230 PubMed24637615

2014

The DNMT1/PCNA/UHRF1 disruption induces tumorigenesis characterized by similar genetic and epigenetic signatures.

Applications

sELISA

Species

Human

Romain Pacaud,Emeline Brocard,Lisenn Lalier,Eric Hervouet,François M Vallette,Pierre-François Cartron
View all publications
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