Human PDGF BB ELISA Kit

Product details

Human PDGF BB ELISA Kit ab184860 is a rapid single-wash 90-min sandwich ELISA kit to measure Human PDGF BB in Plasma (Citrate, EDTA, and Heparin), Cell culture media, and Serum. This Simplestep sensitivity is 2.9 pg/mL.

How the assay works

Human PDGF BB SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

This immunoassay is calibrated against a highly purified human PDGF BB. The NIBSC/WHO unclassified purified human PDGF BB preparation 94/728 was evaluated in this kit.
The dose response curve of the unclassified standard PDGF BB parallels the SimpleStep standard curve.
To convert sample values obtained with the SimpleStep Human PDGF BB kit to approximate NIBSC IU/mL units, use the equation below.

NIBSC (94/728) approximate value (IU/mL) = 0.0114 x SimpleStep Human PDGF BB value (pg/mL).

Does not react with human PDGF-A.

Human PDGF BB ELISA Kit ab184860 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

How other researchers are using Human PDGF BB ELISA Kit ab184860

Human PDGF BB ELISA Kit ab184860 has been used to study factors influencing efficacy of plasma infusion in reproductive medicine⁽¹⁾, characterization of growth factors expressed in head and neck squamous cell carcinomas⁽²⁾, and PDGF BB signaling regulation as a therapuetic strategy in ankylosing spondylitis ⁽³⁾.

References:

⁽¹⁾ P. Lin, et. al., 2023, PMID: 37763186
⁽²⁾ J. Zhou, et. al., 2022, PMID: 36544698
⁽³⁾ S. Jo, et. al., 2023, PMID: 36422470

Related and Recommended products

Human PDGF BB ELISA Kit ab184860 is commonly used to measure PDGF BB as a growth factor essential in regulation of embryonic development, cell proliferation, cell migration, survival, and chemotaxis.

Other ELISA kits to measure related growth factors include:

- Human PDGF BB ELISA Kit, Fluorescent ab229418
- Human PDGF B ELISA Kit ab300321
- Human PDGF AA ELISA Kit ab284616
- Human PDGF-CC ELISA Kit ab234568
- Human PDGF R alpha ELISA Kit ab242246
- Human PDGFRB ELISA Kit ab252357

Platelet derived growth factor B chain plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. As a homodimer it is a potent mitogen for cells of mesenchymal origin and is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGF-BB is essential for normal blood vessel development, for normal development of kidney glomeruli and in wound healing. Signaling is modulated by the formation of heterodimers with PDGF A chain to yield PDGF-AB.