Human PDGF BB ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human PDGF BB with a sensitivity of 0.65 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.69 - 1500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.65 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
Alternative names
PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis
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Human PDGF BB ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human PDGF BB with a sensitivity of 0.65 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.69 - 1500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.65 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 5.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 104 | Range 101 - 106 % |
Sample type Serum | Average % = 95 | Range 85 - 100 % |
Sample type EDTA Plasma | Average % = 99 | Range 91 - 107 % |
Sample type Heparin Plasma | Average % = 104 | Range 92 - 114 % |
Sample type Citrate plasma | Average % = 109 | Range 100 - 114 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
PDGF BB in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PDGF BB protein in human serum, plasma, and cell culture supernatant.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Platelet derived growth factor B chain plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. As a homodimer it is a potent mitogen for cells of mesenchymal origin and is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGF-BB is essential for normal blood vessel development, for normal development of kidney glomeruli and in wound healing. Signaling is modulated by the formation of heterodimers with PDGF A chain to yield PDGF-AB.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDGF-B also known as platelet-derived growth factor subunit B or PDGF-BB is a critical protein involved in various cellular processes. Composed of 241 amino acids PDGF-B exhibits a molecular mass of approximately 27 kDa. Scientists recognize this protein for its expression primarily in platelets but it also appears in a range of other tissues including smooth muscle cells and fibroblasts. The PDGF-B protein plays a substantial role in the regulation of growth and division inducing pathways important for cellular proliferation.
- Biological function summary
PDGF-B influences cellular signaling processes and facilitates interactions that are a part of the PDGF receptor system. PDGF-B can form a homo-dimerization commonly known as the PDGF-BB dimer which is an active form. When PDGF-B binds to its receptor specifically the PDGF receptor β (PDGFR-β) it triggers pathways that control various cellular activities including growth and survival. The interaction of PDGF-B with the receptor is an important step that modulates several downstream signaling pathways.
- Pathways
PDGF-B activates significant pathways such as the MAPK/ERK and PI3K/AKT pathways. These pathways play a major role in the regulation of cell proliferation and differentiation. Through these pathways PDGF-B exhibits interactions with proteins like Ras and Akt which are integral in transmitting signals within the cell. These cascades result in the promotion of processes such as angiogenesis and wound healing highlighting the importance of PDGF-B in cellular function.
- Associated diseases and disorders
Researchers have linked PDGF-B to pathologies such as cancer and atherosclerosis. For instance overexpression of PDGF-B can lead to abnormal vascular growth contributing to tumor development in cancers. It also plays a role in the progression of atherosclerosis by promoting the migration and proliferation of smooth muscle cells leading to plaque formation. In these contexts PDGF-B shows interaction with other proteins such as VEGF in tumor angiogenesis and LDL in atherosclerotic developments reinforcing its involvement in these conditions.
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6 product images
Sandwich ELISA - Human PDGF BB ELISA Kit, Fluorescent (ab229418)
Example of human PDGF BB standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PDGF BB ELISA Kit, Fluorescent (ab229418)
Titration of human serum within the working range of the assay.
Background subtracted data from duplicate measurements are plotted.
Sandwich ELISA - Human PDGF BB ELISA Kit, Fluorescent (ab229418)
Observed PDGF-BB levels in pooled donor normal human plasma and normal human platelet-poor plasma (PP-Plasma).
Mean human PDGF-BB values fall within expected normal reference ranges (WHO).
Sandwich ELISA - Human PDGF BB ELISA Kit, Fluorescent (ab229418)
Observed PDGF-BB levels in individual donor normal Human serum (n=10).
Mean human PDGF-BB is 145 pg/mL and values fall within expected normal reference ranges (WHO).
Sandwich ELISA - Human PDGF BB ELISA Kit, Fluorescent (ab229418)
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.
Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Conditioned media was harvested after 48 hours aliquoted and assayed for endogenous PDGF-BB levels.
Sandwich ELISA - Human PDGF BB ELISA Kit, Fluorescent (ab229418)
The PDGF-BB SimpleStep ELISA was validated for assay specificity against PDGF-A protein.
Background-subtracted data values (mean +/- SD) are graphed. In addition, PDGF-A was prepared at 15 ng/mL and assayed for cross reactivity. No cross reactivity or interference was observed.
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