AB326303

Human PDGFRB ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA
Recombinant
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Human PDGFRB ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human PDGFRB with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 19.2 - 42000 pg/ml with a sensitivity of 5.57pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

CD140b, PDGFR, PDGFR1, PDGFRB, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1

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Sandwich ELISA - Human PDGFRB ELISA Kit, Chemiluminescent (AB326303)

sELISA

Lab

Sandwich ELISA - Human PDGFRB ELISA Kit, Chemiluminescent (AB326303)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Lab

Sandwich ELISA - Human PDGFRB ELISA Kit, Chemiluminescent (AB326303)

Standard curve comparison between the Chemiluminescent Human PDGFRB SimpleStep ELISA and colorimetric Human PDGFRB SimpleStep ELISA (ab252357) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human PDGFRB ELISA Kit, Chemiluminescent (AB326303)

Dynamic range comparison between the Chemiluminescent Human PDGFRB SimpleStep ELISA, colorimetric Human PDGFRB SimpleStep ELISA (ab252357), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

sELISA

Unknown

Sandwich ELISA - Human PDGFRB ELISA Kit, Chemiluminescent (AB326303)

Interpolated concentrations of native PDFGRB in human serum and plasma samples.

The concentrations of PDFGRB were measured in duplicates interpolated from the PDFGRB standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25% plasma (citrate) 25% plasma (EDTA) 12.5% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean PDFGRB concentration was determined to be 35.58 ng/mL in serum 28.31 ng/mL in plasma (citrate) 29.73 ng/mL plasma (EDTA) and 33.26 ng/mL plasma (EDTA).

sELISA

Unknown

Sandwich ELISA - Human PDGFRB ELISA Kit, Chemiluminescent (AB326303)

Interpolated concentrations of native PDFGRB in human kidney extract (250 ug/mL) testis extract (500 ug/mL) and placenta extract (125 ug/mL) based on sample load.

The concentrations of PDFGRB were measured in duplicate and interpolated from the PDFGRB standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean PDFGRB concentration was determined to be 3.50 ng/mL in kidney extract 6.53 ng/mL in testis extract and 1.08 ng/mL in placenta extract.

sELISA

Unknown

Sandwich ELISA - Human PDGFRB ELISA Kit, Chemiluminescent (AB326303)

Serum 10% from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean PDFGRB concentration was determined to be 33.7 ng/mL with a range of 17.28 – 67.41 ng/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.57 pg/mL

Range

19.2 - 42000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human PDGFRB ELISA Kit, Chemiluminescent (ab326303) is a rapid single-wash sandwich 55-min ELISA to measure Human PDGFRB in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Media, Tissue Extract with a sensitivity of 5.57 pg/mL.

How the assay works

Human PDGFRB ELISA Kit, Chemiluminescent (ab326303) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human PDGFRB (ab326303) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PDGF R beta antibody [EPR22290-256] - BSA and Aside free (Capture) ab259649
- Anti-PDGF R beta antibody [EPR22290-54] - BSA and Aside free (Detector) ab259650

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.4" } ]

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Recovery

[ { "sample": "Serum", "range": "84 - 97 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "83 - 92 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "92 - 104 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "92 - 96 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "89 - 100 %", "average": "= 94" }, { "sample": "Tissue Extracts", "range": "83 - 93 %", "average": "= 88" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

See full target information PDGFRB

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websiteProtocolBooklet

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Product promise

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