AB246535

Human PEDF ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Human PEDF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PEDF with a sensitivity of 146 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

View Alternative Names

PEDF, PIG35, SERPINF1, Pigment epithelium-derived factor, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1

4 Images

ELISA

Supplier Data

ELISA - Human PEDF ELISA Kit (AB246535)

sELISA

Supplier Data

Sandwich ELISA - Human PEDF ELISA Kit (AB246535)

Interpolated concentrations of native PEDF in human serum, plasma-citrate and HEPG2 cell culture supernatant samples.

The concentrations of PEDF were measured in duplicates, interpolated from the PEDF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1000, plasma (citrate) 1 : 1000, and HEPG2 cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PEDF concentration was determined to be 22.3 μg/mL in serum, 19.87 μg/mL in plasma (citrate) and 0.448 μg/mL in and HEPG2 cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human PEDF ELISA Kit (AB246535)

Example of human PEDF standard curve in Sample Diluent NS with Enhancer.

The PEDF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human PEDF ELISA Kit (AB246535)

Representative image of Human PEDF ELISA Kit (ab246535)

Components shown from left to right :

- Sample diluent NS

- 10X Human PEDF Capture Antibody

- 10X Human PEDF Detector Antibody

- Human PEDF Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 146 pg/mL

Range

468.75 - 30000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human PEDF ELISA Kit ab246535 is a rapid single-wash 90-min Sandwich ELISA to measure Human PEDF in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 146 pg/mL.

How the assay works

Human PEDF SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human PEDF ELISA Kit ab246535 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PEDF antibody [EPR21067-285] - BSA and Azide free (Capture) ab259608
- Anti-PEDF antibody [EPR21067-2] - BSA and Azide free (Detector) ab259608

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 108 %", "average": "= 107" }, { "sample": "Serum", "range": "109 - 115 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "104 - 112 %", "average": "= 109" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab246535-10x96Tests", "assetComponentDetails": [ { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246535-CMP11" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB246535-CMP09" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246535-CMP04" }, { "size":"10 x 600 µL", "name":"10X Human PEDF Capture Antibody", "number":"AB246535-CMP01" }, { "size":"20 x 1 Vial", "name":"Human PEDF Lyophilized Recombinant Protein", "number":"AB246535-CMP03" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB246535-CMP07" }, { "size":"10 x 600 µL", "name":"10X Human PEDF Detector Antibody", "number":"AB246535-CMP02" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246535-CMP05" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB246535-CMP06" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB246535-CMP10" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB246535-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246535-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human PEDF Capture Antibody", "number":"AB246535-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PEDF Lyophilized Recombinant Protein", "number":"AB246535-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246535-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB246535-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246535-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB246535-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB246535-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246535-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246535-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PEDF Detector Antibody", "number":"AB246535-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246535-CMP07", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEDF also known as pigment epithelium-derived factor is a secreted glycoprotein with a mass of approximately 50 kDa. Its expression is highest in the retina and also occurs in several other tissues including liver and brain. PEDF has neurotrophic and neuroprotective properties. The PEDF protein works mechanically by interacting with receptors on cell surfaces to inhibit angiogenesis and promote neuronal survival and differentiation.

Biological function summary

PEDF plays a significant role in controlling angiogenesis due to its ability to inhibit excessive blood vessel formation. This makes PEDF essential in balancing the vasculature and nutrient availability in tissues. While PEDF does not form part of a larger protein complex its activity influences the biological interplay between cells by stabilizing the extracellular matrix and influencing cell signaling pathways involved in maintaining tissue homeostasis.

Pathways

PEDF interacts directly within the angiogenesis and neuroprotection pathways influencing cellular processes critical for normal tissue function. In angiogenesis PEDF disrupts the VEGF pathway by serving as an inhibitor thereby restricting uncontrolled vessel growth. It also plays a role in neuroprotection pathways where it cooperates with proteins like nerve growth factor to enhance neuronal survival and repair processes.

PEDF relates significantly to diabetic retinopathy and cancer. PEDF's angiogenesis inhibition has therapeutic implications in diabetic retinopathy where unregulated blood vessel growth leads to vision loss. It also shows potential in cancer therapy by targeting tumor angiogenesis. In both conditions its interaction with VEGF is notable as it competes to modulate angiogenesis with potential consequences for disease progression and treatment strategies.

style

left-column

Product protocols

style

left-column

Target data

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

See full target information SERPINF1

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 10:19 PubMed40253438

2025

Allogeneic RPE cell suspension manufactured at scale demonstrating preclinical safety and efficacy led to IND approval.

Applications

Unspecified application

Species

Unspecified reactive species

Lalitha Soundararajan,Harshini Surendran,Niharika Patlolla,Rajani Battu,Jonathan Stoddard,Sebastian Arrizabalaga,Zengping Liu,Gopal Lingam,Xinyi Su,Renee C Ryals,Rajarshi Pal

European journal of medical research 29:146 PubMed38429803

2024

Serum proteome analysis identifies a potential biomarker for axial psoriatic arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Chaofan Lu,Fan Yang,Shihao He,Hongxia Yu,Qian Wang,Mengtao Li,Xiaofeng Zeng,Xiaomei Leng

Bioengineering (Basel, Switzerland) 9: PubMed35877348

2022

Microcarrier-Based Culture of Human Pluripotent Stem-Cell-Derived Retinal Pigmented Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed A Faynus,Jeffrey K Bailey,Britney O Pennington,Mika Katsura,Duncan A Proctor,Ashley K Yeh,Sneha Menon,Dylan G Choi,Jane S Lebkowski,Lincoln V Johnson,Dennis O Clegg

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PEDF ELISA Kit

ELISA - Human PEDF ELISA Kit (AB246535)

Sandwich ELISA - Human PEDF ELISA Kit (AB246535)

Sandwich ELISA - Human PEDF ELISA Kit (AB246535)

Sandwich ELISA - Human PEDF ELISA Kit (AB246535)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Product promise