AB214570

Human Pentraxin 3 / PTX3 ELISA Kit

Name: Human Pentraxin 3 / PTX3 ELISA Kit, 90-min ELISA (ab214570) | Abcam
Brand: Abcam Limited
SKU: AB214570
Price: 805 USD
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
What is this?

(1 Review)

(10 Publications)

Human Pentraxin 3 / PTX3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Pentraxin 3 / PTX3 with a sensitivity of 3.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

TNFAIP5, TSG14, PTX3, Pentraxin-related protein PTX3, Pentaxin-related protein PTX3, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, TNF alpha-induced protein 5, TSG-14

6 Images

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

sELISA

Supplier Data

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Interpolated concentrations of native Pentraxin 3 in human HUVEC Extract sample based on a 200 µg/mL extract load.

The concentrations of Pentraxin 3 were measured in duplicate and interpolated from the Pentraxin 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pentraxin 3 concentration was determined to be 1,368 pg/mL.

sELISA

Lab

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Human Pentraxin 3 standard curve comparison data.

Standard curve comparison between human Pentraxin 3 SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 33-fold increase in sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Interpolated concentrations of native Pentraxin 3 in human plasma, saliva and cell culture supernatant samples.

The concentrations of Pentraxin 3 were measured in duplicates, interpolated from the Pentraxin 3 standard curves and corrected for sample dilution. Undiluted samples are as follows : Plasma (heparin) 25%, plasma (EDTA) 25%, saliva 10%, and HUVEC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pentraxin 3 concentration was determined to be 1,056 pg/mL in plasma (heparin), 1,329 og/mL in plasma (EDTA), 962 pg/mL in saliva and 334 pg/mL in HUVEC cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Example of human Pentraxin 3 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Interpolated concentrations of spiked Pentraxin 3 in human serum and plasma samples.

sELISA

Supplier Data

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Example of human Pentraxin 3 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.4 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Pentraxin 3 / PTX3 ELISA Kit (ab214570) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pentraxin 3 / PTX3 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, tissue extracts, and saliva. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Pentraxin 3 / PTX3 with 3.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Pentraxin 3 (also known as pentraxin-related protein PTX3 or TSG-14) is a secreted protein with a role in innate immune response. It is secreted by many cell types during infectious situations. The standard protein provided in this kit corresponds to the C-terminal Pentaxin domain of Pentraxin 3.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma EDTA", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Intra", "sample": "Plasma EDTA", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 105 %", "average": "= 100" }, { "sample": "Serum", "range": "100 - 108 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "80 - 85 %", "average": "= 82" }, { "sample": "Saliva", "range": "88 - 95 %", "average": "= 92" }, { "sample": "Cell culture extracts", "range": "93 - 103 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "98 - 100 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "94 - 106 %", "average": "= 101" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pentraxin 3 (PTX3) also known as TSG-14 or TNF-Inducible Gene 14 Protein is an important component of the immune system. Mechanically PTX3 functions as part of the body's defense mechanism against pathogens and tissue damage. It has a molecular weight of approximately 42 kDa and is expressed in a range of tissues such as the immune cells endothelium and epithelial cells. Upon inflammatory signals these cells secrete PTX3 which quickly identifies microbial molecules and apoptotic cells.

Biological function summary

The role of PTX3 extends beyond simple pathogen recognition. It is involved in the regulation of inflammation and tissue homeostasis. As part of the pentraxin superfamily PTX3 plays a role in the formation of complexes with other molecules such as complement components. These interactions modulate the complement cascade an essential part of the innate immune response and influence the resolution of inflammation. PTX3 also interacts with extracellular matrix components stabilizing tissue structure during immune response.

Pathways

PTX3 integrates into vital immune signaling networks. It actively participates in the complement pathway where it regulates the activity of C1q and C3 key complement proteins. Additionally PTX3 connects with the Toll-like receptor (TLR) signaling pathway. This interaction helps modulate the body's response to microbial infections by influencing the activity of TLR4 among other related proteins which are fundamental in recognizing pathogen-associated molecular patterns.

PTX3 is linked to various inflammatory conditions. High levels of PTX3 often indicate a response to cardiovascular diseases such as atherosclerosis. In this context its interactions with C1q highlight its role in modulating disease progression. PTX3 also relates to autoimmune diseases like rheumatoid arthritis where its increased expression correlates with disease severity. In rheumatoid arthritis PTX3 can interact with pro-inflammatory cytokines like TNF-alpha exacerbating the inflammatory process.

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Product protocols

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Target data

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.

See full target information PTX3

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37569579

2023

Innate and Adaptive Immunity-Related Markers as Predictors of the Short-Term Progression of Subclinical Atherosclerosis in Middle-Aged Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Vadim Genkel,Ilya Dolgushin,Albina Savochkina,Karina Nikushkina,Irina Baturina,Anna Minasova,Veronika Sumerkina,Lubov Pykhova,Semen Kupriyanov,Alla Kuznetsova,Igor Shaposhnik

Ophthalmology science 3:100278 PubMed36950301

2023

High Levels of C-Reactive Protein with Low Levels of Pentraxin 3 as Biomarkers for Central Serous Chorioretinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Elodie Bousquet,Camille Chenevier-Gobeaux,Thara Jaworski,Héloïse Torres-Villaros,Marta Zola,Irmela Mantel,Laura Kowalczuk,Alexandre Matet,Alejandra Daruich,Min Zhao,Suzanne Yzer,Francine Behar-Cohen

Cancer science 113:4311-4326 PubMed36074525

2022

Pentraxin 3 is an adipose tissue-related serum marker for pancreatic cancer cachexia predicting subsequent muscle mass and visceral fat loss.

Applications

Unspecified application

Species

Unspecified reactive species

Katsuhiko Sato,Hayato Hikita,Minoru Shigekawa,Seiya Kato,Yoichi Sasaki,Kazuma Shinkai,Makoto Fukuoka,Shinnosuke Kudo,Yu Sato,Kenji Fukumoto,Kumiko Shirai,Yuta Myojin,Sadatsugu Sakane,Kazuhiro Murai,Teppei Yoshioka,Akira Nishio,Takahiro Kodama,Ryotaro Sakamori,Tomohide Tatsumi,Tetsuo Takehara

EBioMedicine 80:104077 PubMed35644124

2022

Dynamics of circulating calprotectin accurately predict the outcome of moderate COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Chapuis,Nusaibah Ibrahimi,Thibaut Belmondo,Claire Goulvestre,Anne-Emmanuelle Berger,Alice-Andrée Mariaggi,Muriel Andrieu,Camille Chenevier-Gobeaux,Arnaud Bayle,Lydia Campos,Cherifa Cheurfa,Richard Chocron,Jean-Luc Diehl,Benoît Doumenc,Jérôme Duchemin,Manon Duprat,Fabien François,Nicolas Gendron,Tristant Mirault,Frédéric Pène,Aurélien Philippe,Fanny Pommeret,Olivier Sanchez,David M Smadja,Tali-Anne Szwebel,Aymeric Silvin,Florent Ginhoux,Ludovic Lacroix,Gérôme Jules-Clément,Sarobidy Rapeteramana,Colette Mavier,Laura Steller,Barbara Perniconi,Fabrice André,Damien Drubay,Michaela Fontenay,Sophie Hüe,Stéphane Paul,Eric Solary

Blood purification 52:41-53 PubMed35512641

2022

The Role of Uremic Retention Solutes in the MIA Syndrome in Hemodialysis Subjects.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Ciceri,Luisa Artioli,Lorenza Magagnoli,Alessandra Barassi,Jean-Claude Alvarez,Ziad A Massy,Andrea Galassi,Mario Cozzolino

Medicine 100:e25937 PubMed34128842

2021

Correlation between single nucleotide polymorphisms in the 3 primer untranslated region of PTX3 and the risk of essential hypertension: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Wanwan Chen,Yanmei Liu,Hongyi Pan,Jie Jiang,Huaqing Xiang,Linlin Peng

Nature communications 12:3406 PubMed34099652

2021

SARS-CoV-2 RNAemia and proteomic trajectories inform prognostication in COVID-19 patients admitted to intensive care.

Applications

Unspecified application

Species

Unspecified reactive species

Clemens Gutmann,Kaloyan Takov,Sean A Burnap,Bhawana Singh,Hashim Ali,Konstantinos Theofilatos,Ella Reed,Maria Hasman,Adam Nabeebaccus,Matthew Fish,Mark Jw McPhail,Kevin O'Gallagher,Lukas E Schmidt,Christian Cassel,Marieke Rienks,Xiaoke Yin,Georg Auzinger,Salvatore Napoli,Salma F Mujib,Francesca Trovato,Barnaby Sanderson,Blair Merrick,Umar Niazi,Mansoor Saqi,Konstantina Dimitrakopoulou,Rafael Fernández-Leiro,Silke Braun,Romy Kronstein-Wiedemann,Katie J Doores,Jonathan D Edgeworth,Ajay M Shah,Stefan R Bornstein,Torsten Tonn,Adrian C Hayday,Mauro Giacca,Manu Shankar-Hari,Manuel Mayr

Antioxidants (Basel, Switzerland) 9: PubMed32012711

2020

Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects.

Applications

Unspecified application

Species

Unspecified reactive species

Brisamar Estébanez,Alexandra L Rodriguez,Nishant P Visavadiya,Michael Whitehurst,María J Cuevas,Javier González-Gallego,Chun-Jung Huang

Journal of autoimmunity 106:102336 PubMed31601476

2019

Complement C1q synergizes with PTX3 in promoting NLRP3 inflammasome over-activation and pyroptosis in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xun-Yao Wu,Ke-Tian Li,Hua-Xia Yang,Bo Yang,Xin Lu,Li-Dan Zhao,Yun-Yun Fei,Hua Chen,Li Wang,Jing Li,Ling-Yi Peng,Wen-Jie Zheng,Yong Hou,Ying Jiang,Qun Shi,Wen Zhang,Feng-Chun Zhang,Jian-Min Zhang,Bo Huang,Wei He,Xuan Zhang

Medical science monitor : international medical jo 24:4427-4432 PubMed29949524

2018

Pentraxin-3 in Thrombolytic Therapy for Acute Ischemic Stroke: No Relation with Curative Effect and Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yang Zhang,He-Dong Han,Si-Yang Wang,Shi-Ren Huang,Ben-Qiang Deng

View all publications

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Product promise

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Human Pentraxin 3 / PTX3 ELISA Kit

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Sandwich ELISA - Human Pentraxin 3 / PTX3 ELISA Kit (AB214570)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise