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AB309405

Human Pepsinogen I ELISA Kit

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Human Pepsinogen I ELISA Kit is a Sandwich ELISA for the measurement of Human Pepsinogen I in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Pepsin A-4, Pepsinogen-4, PGA4

1 Images
Sandwich ELISA - Human Pepsinogen I ELISA Kit (AB309405)
  • sELISA

Supplier Data

Sandwich ELISA - Human Pepsinogen I ELISA Kit (AB309405)

Example of standard curve using ab309405. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 20 pg/mL

Range

20 - 6000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human Pepsinogen I ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Pepsinogen I coated on a 96-well plate.

Recovery

[ { "sample": "Serum", "range": "97 - 117 %", "average": "= 106.5" }, { "sample": "Plasma", "range": "89 - 109 %", "average": "= 98.46" }, { "sample": "Cell culture supernatant", "range": "82 - 94 %", "average": "= 88.33" }, { "sample": "Cell culture media", "range": "82 - 94 %", "average": "= 88.33" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309405-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human Pepsinogen I Antibody-coated ELISA Plate", "number":"AB309405-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309405-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB309405-CMP13", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309405-CMP11", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309405-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309405-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Pepsinogen I Protein Standard", "number":"AB309405-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Pepsinogen I Detection Antibody", "number":"AB309405-CMP01", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pepsin is an enzyme with the main function of breaking down proteins into peptides in the stomach. Commonly known as a protease its molecular mass is approximately 35 kDa. This enzyme arises from its inactive precursor pepsinogen which activates in the acidic environment of the stomach. Expression predominantly occurs in the gastric chief cells. Pepsin remains a critical player in protein digestion with pepsin solutions often utilized for in vitro digestion studies.
Biological function summary

Pepsin fulfills an important role in the digestive process by breaking down larger protein molecules into smaller peptides facilitating nutrient absorption. It does not form part of a larger protein complex but operates independently within the gastric environment. Pepsin's enzymatic action targets specific peptide bonds ensuring efficient degradation of dietary proteins into absorbable forms. The specificity of its substrate binding ensures effective digestion and subsequent utilization of amino acids.

Pathways

Pepsin's activity integrates into proteolytic pathways particularly the catabolic processes in the gastrointestinal tract. It interacts with other digestive enzymes including trypsin and chymotrypsin which further hydrolyze peptides originated by pepsin. These interactions highlight its seamless fit in the digestive enzyme cascade optimized for effective protein turnover and nutrient release into the body. The pepsin pathway coordinates closely with the gastric and duodenal enzymatic processes.

Conditions such as peptic ulcers and Zollinger-Ellison syndrome link closely with excessive or dysregulated pepsin activity. Increased pepsin secretion in response to elevated gastric acid levels contributes to the degradation of the stomach lining aggravating ulcer formation. Alcohol-induced gastric mucosal damage also associates with heightened pepsin activity. Dysfunctional interactions involving pepsin and other gastric enzymes such as pepsinogen underline the pathological implications within these disorders.

Product protocols

Target data

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
See full target information PGA4
websiteProtocolBooklet
en

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