Human Perforin ELISA Kit (PRF1) is a sandwich ELISA designed to quantify Human Perforin (PRF1) with a sensitivity of 40 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 62.5 - 2000 pg/mL
- Cited in over 10 publications
Images
Publications
Filter by
- Science advances 9:eadj99642023Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuoyu Zhong,Matthew Sullivan,Neil Upreti,Roy Chen,Agustin De Ganzó,Kaichun Yang,Shujie Yang,Ke Jin,Ye He,Ke Li,Jianping Xia,Zhiteng Ma,Luke P Lee,Tania Konry,Tony Jun HuangPubMed 38134285
- Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 56:e129972023The level of Tim-3+CD8+ T cells can serve as a potential marker for evaluating the severity of acute graft-versus-host disease after haplo-PBSCT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNannan Pang,Mingkai Yu,Jianli Xu,Hailong Yuan,Gang Chen,Dong Wang,Chunxia Han,Weiguo Wang,Jianbing Ding,Ming JiangPubMed 38126537
- Nature communications 14:74612023Early-to-mid stage idiopathic Parkinson's disease shows enhanced cytotoxicity and differentiation in CD8 T-cells in females.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristophe M Capelle,Séverine Ciré,Fanny Hedin,Maxime Hansen,Lukas Pavelka,Kamil Grzyb,Dimitrios Kyriakis,Oliver Hunewald,Maria Konstantinou,Dominique Revets,Vera Tslaf,Tainá M Marques,Clarissa P C Gomes,Alexandre Baron,Olivia Domingues,Mario Gomez,Ni Zeng,Fay Betsou,Patrick May,Alexander Skupin,Antonio Cosma,Rudi Balling,Rejko Krüger,Markus Ollert,Feng Q HefengPubMed 37985656
- Journal for immunotherapy of cancer 9:2021Combinatorial immunotherapy of N-803 (IL-15 superagonist) and dinutuximab with ex vivo expanded natural killer cells significantly enhances in vitro cytotoxicity against GD2 pediatric solid tumors and in vivo survival of xenografted immunodeficient NSG mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaya Chu,Gaurav Nayyar,Susiyan Jiang,Jeremy M Rosenblum,Patrick Soon-Shiong,Jeffrey T Safrit,Dean A Lee,Mitchell S CairoPubMed 34244307
- Cancer immunology research 8:1273-12862020An IL6-Adenosine Positive Feedback Loop between CD73 γδTregs and CAFs Promotes Tumor Progression in Human Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoming Hu,Pu Cheng,Jun Pan,Shimin Wang,Qiannan Ding,Zhou Jiang,Lu Cheng,Xuan Shao,Liming Huang,Jian HuangPubMed 32847938
- Journal of immunology (Baltimore, Md. : 1950) 204:1373-13852020Blockade of -Glycosylation Promotes Antitumor Immune Response of T Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShigemi Sasawatari et. al.PubMed 31969386
- Stem cell research & therapy 9:1982018Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHandan Sevim et. al.PubMed 30021624
- Frontiers in immunology 8:17362017Dynamic Analysis of Human Natural Killer Cell Response at Single-Cell Resolution in B-Cell Non-Hodgkin Lymphoma.
- Scientific reports 7:882017NKG2D modulates aggravation of liver inflammation by activating NK cells in HBV infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYadong Wang et. al.PubMed 28273905
- Oncoimmunology 5:e10629692016Expression of inhibitory receptors on intratumoral T cells modulates the activity of a T cell-bispecific antibody targeting folate receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJens Schreiner,Daniela S Thommen,Petra Herzig,Marina Bacac,Christian Klein,Andreas Roller,Anton Belousov,Victor Levitsky,Spasenija Savic,Wolfgang Moersig,Franziska Uhlenbrock,Viola A Heinzelmann-Schwarz,Pablo Umana,Pavel Pisa,M von Bergwelt-Baildon,Didier Lardinois,Philipp Müller,Vaios Karanikas,Alfred ZippeliusPubMed 27057429
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 2000 pg/mL
- Assay time
- 2h 45m
- Sensitivity
- < 40 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:20889983, PubMed:21037563, PubMed:24558045, PubMed:9058810, PubMed:9164947). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed:9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed:20889983, PubMed:21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed:20038786, PubMed:20225066, PubMed:24558045, PubMed:32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed:24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).
Alternative names
PFP, PRF1, Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein
What's included?
Recommended products
Human Perforin ELISA Kit (PRF1) is a sandwich ELISA designed to quantify Human Perforin (PRF1) with a sensitivity of 40 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 62.5 - 2000 pg/mL
- Cited in over 10 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 2000 pg/mL
- Assay time
- 2h 45m
- Assay Platform
- Microplate
- Sensitivity
- < 40 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 8 | mean 416 pg/mL | SD 20 | C.V. 5 |
Sample Sample B | n 8 | mean 674 pg/mL | SD 25 | C.V. 4 |
Sample Sample C | n 8 | mean 984 pg/mL | SD 24 | C.V. 3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 9 | mean 580 pg/mL | SD 15 | C.V. 3 |
Sample Sample B | n 9 | mean 665 pg/mL | SD 31 | C.V. 5 |
Sample Sample C | n 9 | mean 1004 pg/mL | SD 45 | C.V. 5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 71 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's Perforin (PRF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Perforin in supernatant and buffered solutions.
A monoclonal antibody specific for Perforin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Perforin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Perforin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Perforin present in the samples.
This kit will recognize both endogenous and recombinant Human Perforin.
Please note this kit is not suitable for use with serum and plasma.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Perforin also referred to as PRF1 is a pore-forming protein that plays an important role in immune response. It appears as a 67-kDa protein and is mainly expressed in cytotoxic T cells and natural killer (NK) cells. The function of perforin involves the formation of transmembrane pores in the membranes of target cells aiding in the delivery of pro-apoptotic molecules. This mechanism is essential for the destruction of virus-infected cells and tumor cells. Techniques like perforin IHC and perforin ELISA are suitable for detecting and quantifying this protein in various samples.
- Biological function summary
Perforin helps the immune system respond effectively against infected or malignant cells. It works in conjunction with granzymes forming a complex that perforates target cell membranes allowing granzymes to enter and induce apoptosis. Perforin's role in immune surveillance prevents the propagation of harmful cells within the body. Deficiency or malfunction in perforin can lead to compromised immune function or immune cell dysregulation.
- Pathways
Perforin integrates into the cytotoxic T lymphocyte (CTL) and NK cell-mediated cytotoxicity pathways. These pathways include critical interactions with proteins like granzymes and Fas ligand (FasL) which orchestrate the apoptosis of abnormal cells. Through these pathways perforin ensures the body's ability to identify and eliminate threats. The granule exocytosis pathway in particular heavily depends on perforin's activity to execute effective immune responses.
- Associated diseases and disorders
Mutations or defects in perforin are linked to familial hemophagocytic lymphohistiocytosis (FHL) and some types of cancer. These conditions arise when perforin's ability to function correctly is impaired. For instance in FHL defective perforin hampers the immune cells' ability to kill target cells leading to uncontrolled immune activation. Furthermore in the context of cancer the proper functioning of perforin alongside proteins like granzyme B is critical for the surveillance and elimination of tumor cells.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
ELISA - Human Perforin ELISA Kit (PRF1) (ab46068)
Perforin measured in cell culture supernatants (tested at 1/1-1/100 dilution range; duplicates +/- SD).
ELISA - Human Perforin ELISA Kit (PRF1) (ab46068)
Standard curve with background signal subtracted (duplicates; +/- SD).
Sandwich ELISA - Human Perforin ELISA Kit (PRF1) (ab46068)
Representative standard curve using ab46068
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com