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AB83709

Human Perforin ELISA Set (without plates)

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(2 Publications)

Human Perforin ELISA Set (without plates) is a Sandwich ELISA for the measurement of Human Perforin ELISA Set (without plates) in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

PFP, PRF1, Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein

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Sandwich ELISA - Human Perforin ELISA Set (without plates) (AB83709)
  • sELISA

Supplier Data

Sandwich ELISA - Human Perforin ELISA Set (without plates) (AB83709)

Perforin Standard curve ranging from 62.5 to 2000 pg/mL

Key facts

Sample types

Cell culture supernatant, Serum, Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 40 pg/mL

Range

62.5 - 2000 pg/mL

Assay time

2h 45m

Assay Platform

Reagents

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Primary Antibodies

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  • 5
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Reactivity data

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Product details

Abcam's Perforin Human in vitro ELISA Set is designed for the quantitative measurement of Perforin in cell culture supernatants and buffered solutions.

The Perforin ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step. This ELISA set is intended to measure Perforin in cell -culture supernatants, as well as in serum samples*. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

*Special note for serum & plasma quantification: high and non linear detection level is found in human serum and plasma samples. The high signal appears not dependant to the antibody pairs but probably to the matrix and/ or perforin interaction with other molecules. Consequently serum and plasma quantification is under the scientist responsibility and specific investigations. Remark: no detection signal was found in fetal calf serum, rat, mouse and horse serum

Sensitivity: < 40 pg/ml

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Perforin also referred to as PRF1 is a pore-forming protein that plays an important role in immune response. It appears as a 67-kDa protein and is mainly expressed in cytotoxic T cells and natural killer (NK) cells. The function of perforin involves the formation of transmembrane pores in the membranes of target cells aiding in the delivery of pro-apoptotic molecules. This mechanism is essential for the destruction of virus-infected cells and tumor cells. Techniques like perforin IHC and perforin ELISA are suitable for detecting and quantifying this protein in various samples.
Biological function summary

Perforin helps the immune system respond effectively against infected or malignant cells. It works in conjunction with granzymes forming a complex that perforates target cell membranes allowing granzymes to enter and induce apoptosis. Perforin's role in immune surveillance prevents the propagation of harmful cells within the body. Deficiency or malfunction in perforin can lead to compromised immune function or immune cell dysregulation.

Pathways

Perforin integrates into the cytotoxic T lymphocyte (CTL) and NK cell-mediated cytotoxicity pathways. These pathways include critical interactions with proteins like granzymes and Fas ligand (FasL) which orchestrate the apoptosis of abnormal cells. Through these pathways perforin ensures the body's ability to identify and eliminate threats. The granule exocytosis pathway in particular heavily depends on perforin's activity to execute effective immune responses.

Mutations or defects in perforin are linked to familial hemophagocytic lymphohistiocytosis (FHL) and some types of cancer. These conditions arise when perforin's ability to function correctly is impaired. For instance in FHL defective perforin hampers the immune cells' ability to kill target cells leading to uncontrolled immune activation. Furthermore in the context of cancer the proper functioning of perforin alongside proteins like granzyme B is critical for the surveillance and elimination of tumor cells.
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Product protocols

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Target data

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed : 20889983, PubMed : 21037563, PubMed : 24558045, PubMed : 9058810, PubMed : 9164947). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed : 9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed : 20889983, PubMed : 21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed : 20038786, PubMed : 20225066, PubMed : 24558045, PubMed : 32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed : 24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).
See full target information PRF1
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Publications (2)

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Nature biomedical engineering : PubMed40595437

2025

Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Ma,Huishu Wang,Lunan Liu,Ruiqi Chen,Nandana Mukherjee,Jie Tong,Shadab Kazmi,Xiangyi Fang,Matthew T Witkowski,Iannis Aifantis,Saba Ghassemi,Weiqiang Chen

PLoS pathogens 20:e1012315 PubMed38889192

2024

CD56-mediated activation of human natural killer cells is triggered by Aspergillus fumigatus galactosaminogalactan.

Applications

Unspecified application

Species

Unspecified reactive species

Linda Heilig,Fariha Natasha,Nora Trinks,Vishukumar Aimanianda,Sarah Sze Wah Wong,Thierry Fontaine,Ulrich Terpitz,Lea Strobel,François Le Mauff,Donald C Sheppard,Sascha Schäuble,Oliver Kurzai,Kerstin Hünniger,Esther Weiss,Mario Vargas,P Lynne Howell,Gianni Panagiotou,Sebastian Wurster,Hermann Einsele,Juergen Loeffler
View all publications
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