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AB309314

Human Periostin ELISA Kit

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Human Periostin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Periostin with a sensitivity of 20.98 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

3 Images
Sandwich ELISA - Human Periostin ELISA Kit (AB309314)
  • sELISA

Supplier Data

Sandwich ELISA - Human Periostin ELISA Kit (AB309314)

Interpolated concentration of native Periostin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 80 serum, plasma (citrate), plasma (heparin), and plasma (EDTA).  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Periostin ELISA Kit (AB309314)
  • sELISA

Supplier Data

Sandwich ELISA - Human Periostin ELISA Kit (AB309314)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Periostin concentration was determined to be 123.81 ng/mL with a range of 47.55 – 200.71 ng/mL.

Sandwich ELISA - Human Periostin ELISA Kit (AB309314)
  • sELISA

Supplier Data

Sandwich ELISA - Human Periostin ELISA Kit (AB309314)

Example of human Periostin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Sample types

Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 20.98 pg/mL

Range

78.125 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Periostin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Periostin protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Periostin with 20.98 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.6" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 92 pg/mL", "average": "= 90" }, { "sample": "Citrate plasma", "range": "89 - 106 pg/mL", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "91 - 111 pg/mL", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "78 - 95 pg/mL", "average": "= 87" }, { "sample": "Cell culture media", "range": "101 - 123 %", "average": "= 109" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.
Biological function summary

The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.

Pathways

Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.

The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.
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Product protocols

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Target data

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
See full target information POSTN
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websiteProtocolBooklet
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