AB326736

Human Periostin ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA
Recombinant
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Human Periostin ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Periostin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 10.06 - 22000 pg/ml with a sensitivity of 3.96 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

4 Images

Sandwich ELISA - Human Periostin ELISA Kit, Chemiluminescent (AB326736)

sELISA

Lab

Sandwich ELISA - Human Periostin ELISA Kit, Chemiluminescent (AB326736)

Dynamic range comparison between the Chemiluminescent Human Periostin SimpleStep ELISA, colorimetric Human Periostin SimpleStep ELISA (ab309314), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

sELISA

Lab

Sandwich ELISA - Human Periostin ELISA Kit, Chemiluminescent (AB326736)

Standard curve comparison between the Chemiluminescent Human Periostin SimpleStep ELISA and colorimetric Human Periostin SimpleStep ELISA (ab309314) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Periostin ELISA Kit, Chemiluminescent (AB326736)

Interpolated concentrations of Periostin in normal human serum donors

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Periostin concentration was determined to be 123.81 ng/mL with a range of 47.55 – 200.71 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Periostin ELISA Kit, Chemiluminescent (AB326736)

Interpolated concentrations of human Periostin in serum, plasma (citrate), plasma (heparin), and plasma (EDTA)

Interpolated concentration of native Periostin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 80 serum, plasma (citrate), plasma (heparin), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.96 pg/mL

Range

10.06 - 22000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Periostin ELISA Kit, Chemiluminescent (ab326736) is a rapid single-wash sandwich 55-min ELISA to measure Human Periostin in Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Cell culture media with a sensitivity of 3.96 pg/mL.

How the assay works

Human Periostin ELISA Kit, Chemiluminescent (ab326736) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Periostin (ab326736) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-POSTN antibody [EPR23964-16] - BSA and Aside free (Capture) ab277660
- Anti-POSTN antibody [EPR23964-42] - BSA and Aside free (Detector) ab277661

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.6" } ]

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Recovery

[ { "sample": "Serum", "range": "87 - 92 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "89 - 106 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "91 - 111 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "78 - 95 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "101 - 123 %", "average": "= 123" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).

See full target information POSTN

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com