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AB213816

Human Periostin ELISA Kit (OSF2)

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(3 Publications)

Human Periostin ELISA Kit (OSF2) is a Sandwich (quantitative) ELISA for the measurement of Human Periostin (OSF2) in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

1 Images
Sandwich ELISA - Human Periostin ELISA Kit (OSF2) (AB213816)
  • sELISA

Supplier Data

Sandwich ELISA - Human Periostin ELISA Kit (OSF2) (AB213816)

Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Periostin Enzyme-Linked Immunosorbent Assay (ELISA) kit (OSF2) (ab213816) is designed for the quantitative measurement of Human Periostin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Periostin/OSF2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: N22-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin/OSF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Periostin/OSF2 amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "188 pg/mL", "standardDeviation": "8.64", "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1707 pg/mL", "standardDeviation": "124.61", "coefficientOfVariability": "= 7.3" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4824 pg/mL", "standardDeviation": "192.96", "coefficientOfVariability": "= 4" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4867 pg/mL", "standardDeviation": "199.54", "coefficientOfVariability": "= 4.1" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "180 pg/mL", "standardDeviation": "11.34", "coefficientOfVariability": "= 8.2" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1664 pg/mL", "standardDeviation": "136.44", "coefficientOfVariability": "= 8.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213816-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213816-CMP03", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213816-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213816-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213816-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213816-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213816-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Periostin coated Microplate (12 x 8 wells)", "number":"AB213816-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human Periostin antibody", "number":"AB213816-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213816-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213816-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human Periostin standard", "number":"AB213816-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.
Biological function summary

The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.

Pathways

Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.

The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.
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Product protocols

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Target data

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
See full target information POSTN
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed37371758

2023

Apabetalone Downregulates Fibrotic, Inflammatory and Calcific Processes in Renal Mesangial Cells and Patients with Renal Impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Dean Gilham,Sylwia Wasiak,Brooke D Rakai,Li Fu,Laura M Tsujikawa,Christopher D Sarsons,Agostina Carestia,Kenneth Lebioda,Jan O Johansson,Michael Sweeney,Kamyar Kalantar-Zadeh,Ewelina Kulikowski

Hepatology (Baltimore, Md.) 77:530-545 PubMed36069569

2022

Serum biomarkers correlated with liver stiffness assessed in a multicenter study of pediatric cholestatic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel H Leung,Sridevi Devaraj,Nathan P Goodrich,Xinpu Chen,Deepthi Rajapakshe,Wen Ye,Victor Andreev,Charles G Minard,Danielle Guffey,Jean P Molleston,Lee M Bass,Saul J Karpen,Binita M Kamath,Kasper S Wang,Shikha S Sundaram,Philip Rosenthal,Patrick McKiernan,Kathleen M Loomes,M Kyle Jensen,Simon P Horslen,Jorge A Bezerra,John C Magee,Robert M Merion,Ronald J Sokol,Benjamin L Shneider,Estella Alonso,Lee Bass,Susan Kelly,Mary Riordan,Hector Melin-Aldana,Jorge Bezerra,Kevin Bove,James Heubi,Alexander Miethke,Greg Tiao,Julie Denlinger,Erin Chapman,Ronald Sokol,Amy Feldman,Cara Mack,Michael Narkewicz,Frederick Suchy,Shikha S Sundaram,Johan Van Hove,Benigno Garcia,Mikaela Kauma,Kendra Kocher,Matthew Steinbeiss,Mark Lovell,Kathleen M Loomes,David Piccoli,Elizabeth Rand,Pierre Russo,Nancy Spinner,Jessi Erlichman,Samantha Stalford,Dina Pakstis,Sakya King,Robert Squires,Rakesh Sindhi,Veena Venkat,Kathy Bukauskas,Patrick McKiernan,Lori Haberstroh,James Squires,Philip Rosenthal,Laura Bull,Joanna Curry,Camille Langlois,Grace Kim,Jeffery Teckman,Vikki Kociela,Rosemary Nagy,Shraddha Patel,Jacqueline Cerkoski,Jean P Molleston,Molly Bozic,Girish Subbarao,Ann Klipsch,Cindy Sawyers,Oscar Cummings,Simon P Horslen,Karen Murray,Evelyn Hsu,Kara Cooper,Melissa Young,Laura Finn,Binita M Kamath,Vicky Ng,Claudia Quammie,Juan Putra,Deepika Sharma,Aishwarya Parmar,Stephen Guthery,Kyle Jensen,Ann Rutherford,Amy Lowichik,Linda Book,Rebecka Meyers,Tyler Hall,Kasper S Wang,Sonia Michail,Danny Thomas,Catherine Goodhue,Rohit Kohli,Larry Wang,Nisreen Soufi,Daniel Thomas,Saul Karpen,Nitika Gupta,Rene Romero,Miriam B Vos,Rita Tory,John-Paul Berauer,Carlos Abramowsky,Jeanette McFall,Benjamin L Shneider,Sanjiv Harpavat,Paula Hertel,Daniel Leung,Mary Tessier,Deborah Schady,Laurel Cavallo,Diego Olvera,Christina Banks,Cynthia Tsai,Richard Thompson,Edward Doo,Jay Hoofnagle,Averell Sherker,Rebecca Torrance,Sherry Hall,John Magee,Robert Merion,Cathie Spino,Wen Ye

Life sciences 273:119296 PubMed33675897

2021

Reduced sarcoplasmic reticulum Ca ATPase activity underlies skeletal muscle wasting in asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Rizwan Qaisar,Mughal Qayum,Tahir Muhammad
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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