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AB283988

Human Peroxiredoxin 5 ELISA Kit

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(1 Publication)

Human Peroxiredoxin 5 ELISA Kit is a Sandwich ELISA for the measurement of Human Peroxiredoxin 5 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

ACR1, SBBI10, PRDX5, Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin-dependent peroxiredoxin 5, AOEB166, Prx-V

1 Images
Sandwich ELISA - Human Peroxiredoxin 5 ELISA Kit (AB283988)
  • sELISA

Supplier Data

Sandwich ELISA - Human Peroxiredoxin 5 ELISA Kit (AB283988)

Example of Human Peroxiredoxin 5 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.6 ng/mL

Range

3.125 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Anti-Peroxiredoxin 5 antibody [EPR14529(B)]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Peroxiredoxin 5 (PRDX5) ELISA Kit (ab283988) is designed for detection of PRDX5 in human plasma, serum, cell culture, and cell lysate samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human PRDX5 in less than 5 hours. A polyclonal antibody specific for human PRDX5 has been pre-coated onto a 96- well microplate with removable strips. PRDX5 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human PRDX5, which is recognized by a streptavidinperoxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283988-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283988-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB283988-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB283988-CMP04", "productcode":"" }, { "size":"1 x 180 µL", "name":"30X Biotinylated Human Peroxiredoxin 5 Antibody", "number":"AB283988-CMP06", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283988-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB283988-CMP01", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283988-CMP08", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283988-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Peroxiredoxin 5 Microplate", "number":"AB283988-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Peroxiredoxin 5 Standard", "number":"AB283988-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 5 (Prdx5) acts as a peroxidase reducing hydrogen peroxide and organic hydroperoxides and protecting cells from oxidative damage. This protein also known as AOEB166 and PRDX6 has a molecular mass of approximately 17 kDa. Prdx5 is widely expressed across various tissues including lung liver heart kidney and brain where it performs protective and regulatory roles. It localizes within multiple cell compartments such as mitochondria peroxisomes and the cytosol.
Biological function summary

Prdx5 plays a role in reducing oxidative stress which helps to maintain cellular homeostasis. It is part of the large family of peroxiredoxins known for their antioxidant activity. Prdx5 interacts with other antioxidants within the cell to mitigate damage from reactive oxygen species (ROS). Its multifunctionality allows it to act both as an antioxidant and a molecular chaperone under certain stress conditions helping cells adapt to challenging environments.

Pathways

Prdx5 functions within the antioxidant defense system and participates in the regulatory networks that control oxidative stress responses. It is an element of the thioredoxin system working alongside thioredoxin and thioredoxin reductase to neutralize ROS. Additionally it contributes to the signaling pathways of apoptosis by controlling ROS levels which can impact cell viability and proliferation.

The malfunction or dysregulation of Prdx5 associates with cancer and neurodegenerative diseases. Elevated ROS levels can contribute to tumor progression and have been observed in cancerous tissues where Prdx5 may have altered expression. In neurodegeneration such as Alzheimer's disease Prdx5's role in oxidative stress management potentially affects disease progression. Connections exist between Prdx5 and proteins like amyloid-beta in Alzheimer's illustrating its importance in both protective and pathological contexts.
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Product protocols

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Target data

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.
See full target information PRDX5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37443747

2023

Prdx5 in the Regulation of Tuberous Sclerosis Complex Mutation-Induced Signaling Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Judit Bovari-Biri,ElHusseiny Mohamed Mahmoud Abdelwahab,Kitti Garai,Judit E Pongracz
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Product promise

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