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AB189573

Human PF4 ELISA Kit (CXCL4)

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(10 Publications)

Human PF4 ELISA Kit (CXCL4) is a single-wash 90-min Simplestep used to quantify Human PF4 (CXCL4) with a sensitivity of 24.29 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

CXCL4, SCYB4, PF4, Platelet factor 4, PF-4, C-X-C motif chemokine 4, Iroplact, Oncostatin-A

6 Images
ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)
  • ELISA

Supplier Data

ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)
Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)
  • sELISA

Unknown

Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)

Example of human PF4 standard curve in Sample Diluent NS.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)
  • sELISA

Unknown

Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)

0.5% Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 104.38 ng/mL with a range of 27.73 – 353.63 ng/mL.

Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)
  • sELISA

Unknown

Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)

Interpolated concentrations of native PF4 in human serum, plasma and cell culture supernatant samples.

The concentrations of PF4 were measured in duplicates, interpolated from the PF4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2%, plasma (citrate) 0.5%, and plasma (EDTA) 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 249.53 ng/mL in serum, 622.09 ng/mL in plasma (citrate), and 117.28 ng/mL in plasma (EDTA).

Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)
  • sELISA

Unknown

Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)

Interpolated concentrations of native PF4 in human cell culture supernatant samples.

The concentrations of PF4 were measured in duplicates, interpolated from the PF4 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 19.25 ng/mL in PBMC cell culture supernatant.

Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)
  • sELISA

Unknown

Sandwich ELISA - Human PF4 ELISA Kit (CXCL4) (AB189573)

0.5% Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 267.15 ng/mL with a range of 47.05 – 986.18 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 24.29 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PF4 ELISA Kit (CXCL4) ab189573 is a rapid single-wash 90-min Sandwich ELISA to measure Human PF4 (CXCL4)in cell culture media, cell culture supernatant, citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 24.29 pg/mL.

How the assay works

Human PF4 (CXCL4)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human PF4 ELISA Kit (CXCL4) ab189573 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PF4 antibody [EPR7763] - BSA and Azide free (Capture) ab259442
- Anti-PF4 antibody [EPR7762] - BSA and Azide free (Detector) ab259442

Platelet factor-4 (PF4/CXCL4) is a 70-amino acid protein that is released from the alpha-granules of activated platelets whose major physiologic role appears to be inhibiting local antithrombin III activity and promoting coagulation. PF4 (CXCL4) has a role in inflammation and wound repair by acting as a strong chemoattractant for neutrophils, monocytes, and fibroblasts.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 115 %", "average": "= 108" }, { "sample": "Serum", "range": "89 - 99 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "97 - 115 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "85 - 90 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "83 - 100 %", "average": "= 92" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Platelet Factor 4 (PF4) also known as CXCL4 is a member of the chemokine (C-X-C motif) ligand family. This small protein has a molecular weight of approximately 7.8 kDa. PF4 is mainly expressed in megakaryocytes and stored in the alpha granules of platelets. During platelet activation PF4 releases into the bloodstream where it interacts with other proteins and cell types. Researchers measure PF4 levels with methods such as PF4 ELISA to study its expression and functions under various physiological and pathological conditions.
Biological function summary

PF4 plays an important role in modulating the immune response and coagulation processes. PF4 is not part of a stable protein complex but can interact dynamically with heparin on the surface of endothelial cells and glycosaminoglycans. It inhibits the action of heparin promoting clot formation and angiogenesis. PF4 also attracts and activates different types of leukocytes and can regulate inflammatory processes influencing how the immune system responds to injury.

Pathways

PF4 significantly interacts with both the coagulation and inflammation pathways. In the coagulation pathway PF4 acts to neutralize heparin-like molecules thereby enhancing thrombosis. It works closely with proteins such as thrombin and fibrinogen contributing to the blood clot cascade. Within the inflammatory pathway PF4's chemokine activity helps direct leukocytes to sites of tissue damage or infection linking it to cytokines and other chemokines that mediate immune responses.

Researchers associate PF4 with conditions such as thrombosis and autoimmune pathologies. In thrombosis the overexpression of PF4 can lead to excessive clot formation pointing to its interaction with proteins like thrombin. PF4 also plays a role in heparin-induced thrombocytopenia (HIT) a disorder where the immune system mistakenly produces antibodies against heparin-PF4 complexes further indicating its direct involvement with immune and coagulation pathways.
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Product protocols

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Target data

Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation (PubMed : 29930254, PubMed : 9531587). Acts via different functional receptors including CCR1, CXCR3A or CXCR3B (PubMed : 18174362, PubMed : 29930254). Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling (PubMed : 18174362, PubMed : 24469069). Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence (PubMed : 9531587). Chemotactic for neutrophils and monocytes via CCR1 (PubMed : 29930254). Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis (PubMed : 35701499). In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition (PubMed : 34986347). Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity (PubMed : 9395524).
See full target information PF4
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 13:20 PubMed40312746

2025

Proteomic and lipidomic analysis of low-density lipoprotein identifies potential biomarkers of early estrogen receptor-positive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yamama Abu Mohsen,Rachel Twitto-Greenberg,Anna Cohen,Gil S Leichner,Lidor Mahler,Hofit Cohen,Yehuda Kamari,Aviv Shaish,Ayelet Harari,Alicia Leikin-Frenkel,Efrat Glick Saar,Tamar Geiger,Sergey Malitsky,Maxim Itkin,Dror Harats,Rom Keshet

European journal of emergency medicine : official journal of the European Society for Emergency Medicine 31:429-437 PubMed39480645

2024

Biomarkers of COVID-19 short-term worsening: a multiparameter analysis within the prospective multicenter COVIDeF cohort.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Cancella de Abreu,Jacques Ropers,Nathalie Oueidat,Laurence Pieroni,Corinne Frère,Michaela Fontenay,Krystel Torelino,Anthony Chauvin,Guillaume Hekimian,Anne-Geneviève Marcelin,Beatrice Parfait,Florence Tubach,Pierre Hausfater

Clinical research in cardiology : official journal of the German Cardiac Society 114:112-125 PubMed38922424

2024

NET burden in left atrial blood is associated with biomarkers of thrombosis and cardiac injury in patients with enlarged left atria.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberly Martinod,Annika Claessen,Caroline Martens,Krystin Krauel,Leydi Carolina Velásquez Pereira,Jens Witsch,Thilo Witsch

International journal of hematology 116:937-946 PubMed35994163

2022

Plasma biomarkers associated with survival and thrombosis in hospitalized COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

David Cabrera-Garcia,Andrea Miltiades,Peter Yim,Samantha Parsons,Katerina Elisman,Mohammad Taghi Mansouri,Gebhard Wagener,Neil L Harrison

The Journal of clinical investigation 132: PubMed35358095

2022

Neutrophil extracellular traps regulate ischemic stroke brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Frederik Denorme,Irina Portier,John L Rustad,Mark J Cody,Claudia V de Araujo,Chieko Hoki,Matthew D Alexander,Ramesh Grandhi,Mitchell R Dyer,Matthew D Neal,Jennifer J Majersik,Christian C Yost,Robert A Campbell

Molecular nutrition & food research 64:e2000350 PubMed32918853

2020

Mediterranean Diet and Atherothrombosis Biomarkers: A Randomized Controlled Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Álvaro Hernáez,Olga Castañer,Anna Tresserra-Rimbau,Xavier Pintó,Montserrat Fitó,Rosa Casas,Miguel Ángel Martínez-González,Dolores Corella,Jordi Salas-Salvadó,José Lapetra,Enrique Gómez-Gracia,Fernando Arós,Miquel Fiol,Lluis Serra-Majem,Emilio Ros,Ramón Estruch

Annals of palliative medicine 9:2723-2730 PubMed32954739

2020

The anti-CXCL4 antibody depletes CD4(+)CD25(+)FOXP3(+) regulatory T cells in CD4+ T cells from chronic osteomyelitis patients by the STAT5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Huang,Shuyu Ge

Transfusion 59:1539-1548 PubMed30980757

2019

Effect of leukoreduction and pathogen reduction on the hemostatic function of whole blood.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberly A Thomas,Susan M Shea,Mark H Yazer,Philip C Spinella

Journal of neurology, neurosurgery, and psychiatry 90:272-283 PubMed30567722

2018

Platelet abnormalities in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hélèna L Denis,Jérôme Lamontagne-Proulx,Isabelle St-Amour,Sarah L Mason,Jesse W Rowley,Nathalie Cloutier,Marie-Ève Tremblay,Antony T Vincent,Peter V Gould,Sylvain Chouinard,Andrew S Weyrich,Matthew T Rondina,Roger A Barker,Eric Boilard,Francesca Cicchetti

Biomicrofluidics 11:064105 PubMed29204246

2017

Constricted microfluidic devices to study the effects of transient high shear exposure on platelets.

Applications

Unspecified application

Species

Unspecified reactive species

Nesreen Z Alsmadi,Sarah J Shapiro,Christopher S Lewis,Vinit M Sheth,Trevor A Snyder,David W Schmidtke
View all publications
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