Human PGC ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PGC in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
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- Transgenic research 32:305-3192023The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua-Chuan Zheng,Hang Xue,Hong-Zhi Sun,Wen-Jing Yun,Zheng-Guo CuiPubMed 37247123
- Scientific reports 11:207942021A panel of urine-derived biomarkers to identify sepsis and distinguish it from systemic inflammatory response syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Tang,Ning Ling,Shiying Li,Juan Huang,Wenyue Zhang,An Zhang,Hong Ren,Yixuan Yang,Huaidong Hu,Xiaohao WangPubMed 34675320
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 54.69 - 3500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 16.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Version
Alternative names
Gastricsin, Pepsinogen C, PGC
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Human PGC ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PGC in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 54.69 - 3500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 16.5 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 8.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 104 | Range 99 - 108 % |
Sample type EDTA Plasma | Average % = 101 | Range 93 - 107 % |
Sample type Heparin Plasma | Average % = 95 | Range 93 - 98 % |
Sample type Citrate plasma | Average % = 90 | Range 88 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human PGC ELISA kit (ab275552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PGC protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PGC with 16.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Pepsinogen C (PGC, progastricsin) is an aspartic proteinase zymogen that belongs to the peptidase family A1. Pespsin C, the activated protease, is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. Pepsinogen C protein is synthesized as an inactive zymogen that autocatalizes by sequential cleavage of the prosegment into its active mature form at low pH. Pepsinogen C polymorphisms are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pepsinogen II also known as PGC or progastricsin functions as a digestive zymogen activated to pepsin in the acidic environment of the stomach. This enzyme precursor has a molecular mass of approximately 42-47 kDa. It is primarily expressed in the gastric chief cells and mucous cells of the stomach lining. Pepsinogen II plays an important role in the initial digestion of proteins breaking down complex protein molecules into peptides.
- Biological function summary
Pepsinogen II contributes to digestive processes by converting into active pepsin at low pH levels facilitating protein degradation. Unlike some other digestive enzymes pepsin works independently from a complex. The production and secretion of pepsinogen II align with the gastric acid production ensuring efficient digestive activity. Its expression is vital for proper nutritional absorption and maintenance of gastrointestinal health.
- Pathways
Pepsinogen II is closely involved in the proteolytic enzyme pathway. It connects with other gastric enzymes such as pepsin A through the activation processes influenced by gastric acid secretion. This interplay among digestive enzymes ensures optimal protein breakdown and nutrient absorption. The regulatory mechanisms that modulate gastric acid and pepsinogen secretion play an integral role in maintaining the digestive efficiency.
- Associated diseases and disorders
Pepsinogen II levels can serve as biomarkers for certain gastric conditions including gastritis and gastric cancer. Altered levels of pepsinogen II are associated with atrophic gastritis where reduced enzyme levels reflect damage to gastric mucosa. Also increased serum pepsinogen II concentration can indicate potential gastric cancer risk. These conditions highlight pepsinogen II's clinical importance in gastrointestinal disease diagnosis and management.
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2 product images
Sandwich ELISA - Human PGC ELISA Kit (ab275552)
Example of human PGC standard curve in Sample Diluent NS.
The PGC standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PGC ELISA Kit (ab275552)
Interpolated concentrations of native PGC in human serum and plasma samples.
The concentrations of PGC were measured in duplicates, interpolated from the PGC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 50%, and plasma (heparin) 25% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PGC concentration was determined to be 12.02 ng/mL in serum, 8.96 ng/mL in plasma (citrate), 6.89 ng/mL in and plasma (EDTA), and 8.41 ng/mL in plasma (heparin).
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