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Human PGC ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PGC in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Alternative names=Gastricsin, Pepsinogen C, PGC
Colorimetric
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
54.69 - 3500 pg/mL
1h 30m
= 16.5 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Human PGC ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human PGC in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Alternative names=Gastricsin, Pepsinogen C, PGC
Colorimetric
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
54.69 - 3500 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 16.5 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.8 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 8.1 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 104 | Range 99 - 108 % |
Sample type EDTA Plasma | Average % = 101 | Range 93 - 107 % |
Sample type Heparin Plasma | Average % = 95 | Range 93 - 98 % |
Sample type Citrate plasma | Average % = 90 | Range 88 - 94 % |
Blue Ice
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Human PGC ELISA kit (ab275552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PGC protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PGC with 16.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Pepsinogen C (PGC, progastricsin) is an aspartic proteinase zymogen that belongs to the peptidase family A1. Pespsin C, the activated protease, is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. Pepsinogen C protein is synthesized as an inactive zymogen that autocatalizes by sequential cleavage of the prosegment into its active mature form at low pH. Pepsinogen C polymorphisms are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.
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