AB275552

Human PGC ELISA Kit

Recombinant
SimpleStep
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(2 Publications)

Human PGC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PGC with a sensitivity of 16.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Gastricsin, Pepsinogen C, PGC

2 Images

Sandwich ELISA - Human PGC ELISA Kit (AB275552)

sELISA

Supplier Data

Sandwich ELISA - Human PGC ELISA Kit (AB275552)

Interpolated concentrations of native PGC in human serum and plasma samples.

The concentrations of PGC were measured in duplicates, interpolated from the PGC standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 50%, and plasma (heparin) 25% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PGC concentration was determined to be 12.02 ng/mL in serum, 8.96 ng/mL in plasma (citrate), 6.89 ng/mL in and plasma (EDTA), and 8.41 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human PGC ELISA Kit (AB275552)

Example of human PGC standard curve in Sample Diluent NS.

The PGC standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 16.5 pg/mL

Range

54.69 - 3500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human PGC ELISA kit (ab275552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PGC protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PGC with 16.5 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Pepsinogen C (PGC, progastricsin) is an aspartic proteinase zymogen that belongs to the peptidase family A1. Pespsin C, the activated protease, is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. Pepsinogen C protein is synthesized as an inactive zymogen that autocatalizes by sequential cleavage of the prosegment into its active mature form at low pH. Pepsinogen C polymorphisms are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Serum", "range": "99 - 108 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "93 - 107 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "93 - 98 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "88 - 94 %", "average": "= 90" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pepsinogen II also known as PGC or progastricsin functions as a digestive zymogen activated to pepsin in the acidic environment of the stomach. This enzyme precursor has a molecular mass of approximately 42-47 kDa. It is primarily expressed in the gastric chief cells and mucous cells of the stomach lining. Pepsinogen II plays an important role in the initial digestion of proteins breaking down complex protein molecules into peptides.

Biological function summary

Pepsinogen II contributes to digestive processes by converting into active pepsin at low pH levels facilitating protein degradation. Unlike some other digestive enzymes pepsin works independently from a complex. The production and secretion of pepsinogen II align with the gastric acid production ensuring efficient digestive activity. Its expression is vital for proper nutritional absorption and maintenance of gastrointestinal health.

Pathways

Pepsinogen II is closely involved in the proteolytic enzyme pathway. It connects with other gastric enzymes such as pepsin A through the activation processes influenced by gastric acid secretion. This interplay among digestive enzymes ensures optimal protein breakdown and nutrient absorption. The regulatory mechanisms that modulate gastric acid and pepsinogen secretion play an integral role in maintaining the digestive efficiency.

Pepsinogen II levels can serve as biomarkers for certain gastric conditions including gastritis and gastric cancer. Altered levels of pepsinogen II are associated with atrophic gastritis where reduced enzyme levels reflect damage to gastric mucosa. Also increased serum pepsinogen II concentration can indicate potential gastric cancer risk. These conditions highlight pepsinogen II's clinical importance in gastrointestinal disease diagnosis and management.

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Product protocols

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Target data

Hydrolyzes a variety of proteins.

See full target information PGC

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Transgenic research 32:305-319 PubMed37247123

2023

The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Chuan Zheng,Hang Xue,Hong-Zhi Sun,Wen-Jing Yun,Zheng-Guo Cui

Scientific reports 11:20794 PubMed34675320

2021

A panel of urine-derived biomarkers to identify sepsis and distinguish it from systemic inflammatory response syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Tang,Ning Ling,Shiying Li,Juan Huang,Wenyue Zhang,An Zhang,Hong Ren,Yixuan Yang,Huaidong Hu,Xiaohao Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PGC ELISA Kit

Sandwich ELISA - Human PGC ELISA Kit (AB275552)

Sandwich ELISA - Human PGC ELISA Kit (AB275552)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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