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AB267571

Human PGRPs ELISA Kit

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Human PGRPs ELISA Kit is a Sandwich ELISA for the measurement of Human PGRPs in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PGLYRP, PGRP, TNFSF3L, SBBI68, UNQ639/PRO1269, PGLYRP1, Peptidoglycan recognition protein 1, Peptidoglycan recognition protein short, PGRP-S

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Sandwich ELISA - Human PGRPs ELISA Kit (AB267571)
  • sELISA

Supplier Data

Sandwich ELISA - Human PGRPs ELISA Kit (AB267571)

Human PGRPs ELISA kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6 pg/mL

Range

6 - 1500 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PGRPs ELISA Kit is designed for the quantitative determination of PGRPs in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human PGRPs coated on a 96-well plate. Standards and samples are pipetted into the wells and PGRPs present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PGRPs antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PGRPs bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA antibody pair detects human PGRPs, less than 0.05% cross-reactivity with human PGRP-1 beta and mouse PGRPs is observed.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "99 - 128 %", "average": "= 114" }, { "sample": "Plasma", "range": "91 - 119 %", "average": "= 107.7" }, { "sample": "Cell culture media", "range": "83 - 123 %", "average": "= 103.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267571-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267571-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267571-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267571-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267571-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267571-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PGRPs Standard (Lyophilized)", "number":"AB267571-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human PGRPs coated Microplate (12 x 8 wells)", "number":"AB267571-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human PGRPs Detection Antibody", "number":"AB267571-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peptidoglycan Recognition Proteins (PGRPs) are pattern recognition receptors known for their role in innate immunity. Some alternate names for PGRPs include PGLYRPs. These proteins identify bacterial peptidoglycans triggering immune responses. PGRPs have varying molecular masses that range typically between 20 to 25 kDa. Expression of PGRPs occurs in several tissues including skin eyes liver and throughout the gastrointestinal tract.
Biological function summary

Functioning in innate immunity PGRPs bind to bacterial cell wall components such as peptidoglycan and lipopolysaccharides. When PGRPs detect these molecules they can activate immune responses to neutralize pathogens. Some PGRPs can also act as amidases breaking down peptidoglycans. PGRPs do not usually act alone; they are part of complexes with other immune proteins to enhance pathogen recognition and response.

Pathways

PGRPs play significant roles in the immune pathway and inflammatory signaling pathways. They interact with the toll-like receptor pathways to mediate responses to bacterial infections. PGRPs also link with Nod proteins to modulate the immune response upon detecting peptidoglycans. These pathways help coordinate an effective defense against infection.

PGRPs’ malfunction or dysregulation links with inflammatory bowel disease and psoriasis. Altered PGRP levels correlate with changes in the gut flora contributing to inflammatory bowel disease. In psoriasis PGRPs’ improper function may exacerbate skin inflammation because they interact with proteins like IL-6 and TNF-alpha. Such interactions highlight their potential as therapeutic targets for these conditions.
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Product protocols

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Target data

Innate immunity protein that plays several important functions in antimicrobial and antitumor defense systems. Acts as a pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria and thus provides bactericidal activity (PubMed : 9707603). Forms an equimolar complex with heat shock protein HSPA1A and induces programmed cell death through apoptosis and necroptosis in tumor cell lines by activating the TNFR1 receptor on the target cell membrane (PubMed : 21247889, PubMed : 26183779). In addition, acts in complex with the Ca(2+)-binding protein S100A4 as a chemoattractant able to induce lymphocyte movement (PubMed : 26654597). Mechanistically, this complex acts as a ligand of the chemotactic receptors CCR5 and CXCR3 which are present on the cells of the immune system (PubMed : 30713770). Also promotes the activation of lymphocytes that become able to kill virus-infected cells as well as tumor cells by modulating the spectrum of their target-cell specificity (PubMed : 28977785, PubMed : 29083508). Induction of cytotoxicity on monocyte surface requires interaction with TREM1 receptor (PubMed : 25595774, PubMed : 28977785).
See full target information PGLYRP1
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Product promise

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