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AB318932

Human PI 3 Kinase p85 alpha ELISA Kit

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Human PI 3 Kinase p85 alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PI 3 Kinase p85 alpha with a sensitivity of 10 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha

4 Images
Sandwich ELISA - Human PI 3 Kinase p85 alpha ELISA Kit (AB318932)
  • sELISA

Supplier Data

Sandwich ELISA - Human PI 3 Kinase p85 alpha ELISA Kit (AB318932)

Interpolated concentrations of human PI 3 Kinase p85 alpha in HeLa cell extract and human liver tissue extract. Interpolated concentration of native PI 3 Kinase p85 alpha was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 500 µg/mL HeLa cell extract and 184 µg/mL human liver tissue extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human PI 3 Kinase p85 alpha ELISA Kit (AB318932)
  • sELISA

Supplier Data

Sandwich ELISA - Human PI 3 Kinase p85 alpha ELISA Kit (AB318932)

Interpolated concentrations of human PI 3 Kinase p85 alpha in human liver tissue extract in 96-well vs. 384-well plates. Interpolated concentration of native PI 3 Kinase p85 alpha was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 184 µg/mL human liver tissue extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human PI 3 Kinase p85 alpha ELISA Kit (AB318932)
  • sELISA

Supplier Data

Sandwich ELISA - Human PI 3 Kinase p85 alpha ELISA Kit (AB318932)

Example of human PI 3 Kinase p85 alpha standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Example of human PI 3 Kinase p85 alpha standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PI 3 Kinase p85 alpha ELISA Kit (AB318932)
  • sELISA

Supplier Data

Sandwich ELISA - Human PI 3 Kinase p85 alpha ELISA Kit (AB318932)

Example of human PI 3 Kinase p85 alpha standard curve in 1X Cell Extraction Buffer PTR. Example of human PI 3 Kinase p85 alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 10 pg/mL

Range

39.063 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PI 3 Kinase p85 alpha SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PI 3 Kinase p85 alpha protein in Cell Lysate, Tissue extracts. Quantitate Human PI 3 Kinase p85 alpha with 10 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "85 - 88 %", "average": "= 87" }, { "sample": "Cell culture extracts", "range": "88 - 117 %", "average": "= 103" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI 3 Kinase p85 alpha also known as PI3KR1 or phospho PI3K is a regulatory subunit of phosphoinositide 3-kinase (PI3K) that plays a fundamental role in various signaling pathways. The p85 protein has a molecular weight of approximately 85 kDa. This protein is widely expressed in many cell types facilitating essential cellular functions by forming a heterodimer with the class I PI3K catalytic subunit often implicated in regulating lipid signaling pathways.
Biological function summary

P85 alpha modulates intracellular signaling by interacting with other proteins in a complex formation with catalytic subunit p110. This interaction enables the initiation of downstream signaling cascades controlling cell growth proliferation and survival. PI 3 kinase activity mediated by p85 alpha is integral to cellular homeostasis and metabolism helping to execute cellular responses to a variety of growth factors and hormones.

Pathways

PI 3 Kinase p85 alpha integrates into two principal signaling networks: the PI3K-Akt and mTOR pathways. These pathways are instrumental for regulating anabolic processes and cell survival. The p85 form of PI3K interacts with additional proteins such as Akt and mTOR coordinating cellular energy balance promoting protein synthesis and inhibiting apoptosis. Through these interactions the protein ensures that cells respond accurately to nutritional and stress signals.

Alterations in PI 3 Kinase p85 alpha function have been linked to cancer and type 2 diabetes. Aberrant signaling involving PI3K-Akt-mTOR pathways due to mutations in PI3KR1 can contribute to tumor progression and chemoresistance. Moreover dysfunction of this pathway affects insulin signaling connecting p85 alpha to diabetic pathologies. Proteins such as IRS-1 (insulin receptor substrate 1) act alongside p85 alpha in mediating these disease-associated processes emphasizing its importance in maintaining physiological balance.
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Product protocols

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Target data

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed : 17626883, PubMed : 19805105, PubMed : 7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed : 20348923).
See full target information PIK3R1
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB191606

Anti-PI 3 Kinase p85 alpha antibody [EPR18702]

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Proteins & Peptides

AB84769

Recombinant Human PI 3 Kinase p85 alpha protein

Western blot - Recombinant Human PI 3 Kinase p85 alpha protein (AB84769)

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