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AB313923

Human PILRA ELISA Kit

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Human PILRA ELISA Kit is a Sandwich ELISA for the measurement of Human PILRA in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Paired immunoglobulin-like type 2 receptor alpha, Cell surface receptor FDF03, Inhibitory receptor PILR-alpha, PILRA

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Sandwich ELISA - Human PILRA ELISA Kit (AB313923)
  • sELISA

Supplier Data

Sandwich ELISA - Human PILRA ELISA Kit (AB313923)

Example of standard curve using ab313923. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.5 pg/mL

Range

5.5 - 1500 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PILRA ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PILRA in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human PILRA coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL7, CD36, CLEC9a, CL-P1, Dectin-2, DLL4, DSCAM, EDIL3, ENPP-7, Enteropeptidase, FCRL3, FCRLB, FGF-3, FLRT1, FLRT2, GBA3, GDF-11, Glycoprotein V, Granzyme A, IGSF4B, IL-28 R alpha, Kynureninase, LAMA4, LRRC4, LRRTM3, NG2, NQO1, PCSK2, Plexin A4, POGLUT1, PRELP, Smad4, SOX15, SOX7, SOX9, Syntaxin 6, TROP2, TSLP R, UNC5H4.

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Recovery

[ { "sample": "Serum", "range": "99 - 105 %", "average": "= 106.8" }, { "sample": "Plasma", "range": "121 - 146 %", "average": "= 132.4" }, { "sample": "Cell culture media", "range": "104 - 115 %", "average": "= 109.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313923-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313923-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human PILRA Protein Standard", "number":"AB313923-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB313923-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human PILRA Antibody-coated ELISA Plate", "number":"AB313923-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human PILRA Detection Antibody", "number":"AB313923-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313923-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313923-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313923-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PILRA also known as Paired immunoglobulin-like type 2 receptor alpha is a protein with a mass of about 38 kDa. It belongs to the immunoglobulin superfamily and acts as an inhibitory receptor. PILRA is expressed mainly in human tissues such as monocytes macrophages and certain subsets of dendritic cells. It interacts with ligands through its extracellular domain and transmits inhibitory signals via its cytoplasmic tail that contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).
Biological function summary

PILRA plays a significant role in the immune system regulation by inhibiting cellular activation processes. This regulation occurs through its participation in receptor-ligand interactions that modulate the immune response. It is not part of a complex but its pair PILRB acts as an activating receptor providing a balanced modulation of immune signaling. The balance between PILRA and PILRB affects how immune cells respond to various signals.

Pathways

PILRA is centrally involved in immune response pathways especially those controlling immune cell signaling. It interacts with proteins involved in immune system pathways such as LILRB1 which also mediates inhibitory signaling. PILRA functions in signaling pathways to regulate the activation and inhibition of immune responses maintaining immune system homeostasis.

PILRA associates with alterations in immune response mechanisms that can lead to autoimmune diseases and infections. Research links PILRA to diseases such as multiple sclerosis and certain viral infections. In these conditions PILRA's role in immune signaling modulation becomes important as it coordinates with proteins like PILRB to affect the immune system's response to different pathological conditions.
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Product protocols

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Target data

Paired receptors consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. PILRA is thought to act as a cellular signaling inhibitory receptor by recruiting cytoplasmic phosphatases like PTPN6/SHP-1 and PTPN11/SHP-2 via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Receptor for PIANP.. (Microbial infection) Acts as an entry co-receptor for herpes simplex virus 1.
See full target information PILRA
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en
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Product promise

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