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AB309426

Human Placental alkaline phosphatase ELISA Kit

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Human Placental alkaline phosphatase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Placental alkaline phosphatase in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PLAP, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1

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Sandwich ELISA - Human Placental alkaline phosphatase ELISA Kit (AB309426)
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Supplier Data

Sandwich ELISA - Human Placental alkaline phosphatase ELISA Kit (AB309426)

Example of standard curve using ab309426. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.04 ng/mL

Range

0.04 - 12.5 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Placental alkaline phosphatase ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Placental alkaline phosphatase in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Placental alkaline phosphatase coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "79 - 135 %", "average": "= 108.5" }, { "sample": "Plasma", "range": "132 - 139 %", "average": "= 135.9" }, { "sample": "Cell culture media", "range": "89 - 105 %", "average": "= 94.87" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309426-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human ALP Protein Standard", "number":"AB309426-CMP11", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309426-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human ALP Detection Antibody", "number":"AB309426-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309426-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309426-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB309426-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ALP Antibody-coated ELISA Plate", "number":"AB309426-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB309426-CMP12", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309426-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Placental alkaline phosphatase (PLAP) also known by names like alkaline phosphatase/PLAP protein or ALPP functions as an enzyme that removes phosphate groups from molecules. It typically appears in tissues such as the placenta and is known to be active during pregnancy. PLAP has a molecular mass of approximately 66 kDa. Normally found expressed in the placenta this enzyme shares similarities with other alkaline phosphatases but is distinct due to its specific expression and function during gestation.
Biological function summary

PLAP shows importance in bone mineralization and fetal development. It participates in the hydrolysis of phosphate esters influencing nutrient availability and metabolism. PLAP is not a part of major protein complexes; however its enzymatic activity directly affects local phosphate concentrations which are critical for processes like bone formation and tissue regeneration during pregnancy.

Pathways

PLAP expression and activity intersect significantly with phosphate metabolism and fetal development pathways. The alkaline phosphatase family plays central roles in these pathways and PLAP shares functions with other isoenzymes in the family such as the tissue nonspecific alkaline phosphatase. These enzymes regulate phosphate turnover and contribute to essential developmental processes.

PLAP connects primarily to certain types of cancers and gestational disorders. Elevated levels of PLAP link to testicular germ cell tumors and ovarian cancer where it serves as a biomarker. In gestational trophoblastic disease PLAP expression provides valuable diagnostic information. Interactions with proteins like hCG (human chorionic gonadotropin) also offer insights into potential disease mechanisms especially in reproductive health challenges.
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Product protocols

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Target data

Alkaline phosphatase that can hydrolyze various phosphate compounds.
See full target information ALPP
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB133602

Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] (AB133602)

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Proteins & Peptides

AB316533

Recombinant Human Placental alkaline phosphatase (PLAP) Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Placental alkaline phosphatase (PLAP) Protein Standard (His tag) (AB316533)

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] (AB124024)

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Product promise

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