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AB303758

Human Placental alkaline phosphatase (PLAP) ELISA Kit

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Human Placental alkaline phosphatase (PLAP) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Placental alkaline phosphatase (PLAP) with a sensitivity of 20.466 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PLAP, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1

3 Images
Sandwich ELISA - Human Placental alkaline phosphatase (PLAP) ELISA Kit (AB303758)
  • sELISA

Supplier Data

Sandwich ELISA - Human Placental alkaline phosphatase (PLAP) ELISA Kit (AB303758)

Interpolated concentrations of Placental alkaline phosphatase (PLAP) in normal human serum donors

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Placental alkaline phosphatase (PLAP) concentration of detected samples was determined to be 314.4 pg/mL with a range of undetected – 1,672 pg/mL.

Sandwich ELISA - Human Placental alkaline phosphatase (PLAP) ELISA Kit (AB303758)
  • sELISA

Supplier Data

Sandwich ELISA - Human Placental alkaline phosphatase (PLAP) ELISA Kit (AB303758)

Example of human Placental alkaline phosphatase (PLAP) standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Placental alkaline phosphatase (PLAP) ELISA Kit (AB303758)
  • sELISA

Supplier Data

Sandwich ELISA - Human Placental alkaline phosphatase (PLAP) ELISA Kit (AB303758)

Interpolated concentration of native Placental alkaline phosphatase (PLAP) was measured in duplicate at different sample concentrations. Undiluted samples are neat serum, plasma – citrate, plasma – EDTA, and plasma - heparin. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 20.466 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

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Proteins & Peptides

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Placental alkaline phosphatase (PLAP) SimpleStep ELISA® kit (ab303758) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Placental alkaline phosphatase (PLAP) protein in serum, plasma (cit), plasma (hep), plasma (EDTA) and cell culture media. Quantitate Human Placental alkaline phosphatase (PLAP) with 20.466 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 91 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "82 - 88 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "85 - 96 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "85 - 87 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "109 - 116 %", "average": "= 112" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Placental alkaline phosphatase (PLAP) also known by names like alkaline phosphatase/PLAP protein or ALPP functions as an enzyme that removes phosphate groups from molecules. It typically appears in tissues such as the placenta and is known to be active during pregnancy. PLAP has a molecular mass of approximately 66 kDa. Normally found expressed in the placenta this enzyme shares similarities with other alkaline phosphatases but is distinct due to its specific expression and function during gestation.
Biological function summary

PLAP shows importance in bone mineralization and fetal development. It participates in the hydrolysis of phosphate esters influencing nutrient availability and metabolism. PLAP is not a part of major protein complexes; however its enzymatic activity directly affects local phosphate concentrations which are critical for processes like bone formation and tissue regeneration during pregnancy.

Pathways

PLAP expression and activity intersect significantly with phosphate metabolism and fetal development pathways. The alkaline phosphatase family plays central roles in these pathways and PLAP shares functions with other isoenzymes in the family such as the tissue nonspecific alkaline phosphatase. These enzymes regulate phosphate turnover and contribute to essential developmental processes.

PLAP connects primarily to certain types of cancers and gestational disorders. Elevated levels of PLAP link to testicular germ cell tumors and ovarian cancer where it serves as a biomarker. In gestational trophoblastic disease PLAP expression provides valuable diagnostic information. Interactions with proteins like hCG (human chorionic gonadotropin) also offer insights into potential disease mechanisms especially in reproductive health challenges.
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Product protocols

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Target data

Alkaline phosphatase that can hydrolyze various phosphate compounds.
See full target information ALPP
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websiteProtocolBooklet
en
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Product promise

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