Human Plasminogen ELISA Kit (PLG) ab108893 is used to quantify Human Plasminogen with a sensitivity of 0.4 ng/ml.
Sandwich ELISA Kit
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- International journal of hematology 116:937-9462022Plasma biomarkers associated with survival and thrombosis in hospitalized COVID-19 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Cabrera-Garcia,Andrea Miltiades,Peter Yim,Samantha Parsons,Katerina Elisman,Mohammad Taghi Mansouri,Gebhard Wagener,Neil L HarrisonPubMed 35994163
- Transplantation 103:1821-18332019Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura J Zitur,Peter J Chlebeck,Scott K Odorico,Juan S Danobeitia,Tiffany J Zens,Cees Van Kooten,Michael Eerhart,Jose A Reyes,Megan L Springer,Jennifer M Coonen,Kevin G Brunner,Saverio V Capuano,Anthony M D'Alessandro,Luis A FernandezPubMed 30964836
- PloS one 9:e1053652014A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM).
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue, Cell culture supernatant, Serum, Cell Lysate
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
0.625 - 40 ng/mL
- Assay time
3h
- Sensitivity
= 0.26 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
Alternative names
Plasminogen, PLG
What's included?
Recommended products
Human Plasminogen ELISA Kit (PLG) ab108893 is used to quantify Human Plasminogen with a sensitivity of 0.4 ng/ml.
Sandwich ELISA Kit
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue, Cell culture supernatant, Serum, Cell Lysate
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
0.625 - 40 ng/mL
- Assay time
3h
- Assay Platform
Microplate
- Sensitivity
= 0.26 ng/mL
- General recovery
101%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 5.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 9.7 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
Human Plasminogen ELISA kit (PLG) is designed for the quantitative measurement of Plasminogen (PLG) concentrations in Human plasma, serum, urine, saliva, milk, tissue samples, cerebrospinal fluid, cell culture, and cell lysate samples.
A Plasminogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Plasminogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Plasminogen captured in plate.
Get higher sensitivity in only 90 minutes with Human Plasminogen ELISA Kit (ab196262) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plasminogen also known as PLG is an important protein with a molecular weight of approximately 92 kDa. Plasminogen is a zymogen which means it is an inactive precursor that requires activation to function. It is produced primarily in the liver and circulates in blood plasma. Its expression occurs mainly in the liver but you can detect it in various tissues including the kidney. Plasminogen binds to tissue surfaces and undergoes transformation into plasmin an active enzyme that plays significant roles in many biological processes.
- Biological function summary
Plasminogen plays a central role in fibrinolysis which is the breakdown of fibrin clots. This process is essential in wound healing and maintaining normal blood flow. Plasminogen once activated to plasmin acts by degrading fibrin a major component of blood clots. The protein forms part of the fibrinolytic system along with other components like tissue plasminogen activator (tPA). Together they ensure that any clots forming within blood vessels are regulated and dissolved as necessary preventing blockages.
- Pathways
Plasminogen is deeply involved in the coagulation and fibrinolytic systems. The conversion of plasminogen to plasmin is a critical step in these pathways requiring activators such as urokinase. Plasminogen has interactions with proteins like alpha-2-antiplasmin and thrombin which help modulate its activity. These interactions ensure a careful balance is maintained between clot formation and dissolution which is vital for normal bodily function.
- Associated diseases and disorders
Plasminogen deficiency is linked with conditions like ligneous conjunctivitis a rare disorder characterized by membranous lesions mainly in the eyes. Reduced plasminogen activity can lead to excessive clot formation contributing to thrombosis risk. Plasminogen's interaction with plasmin and its deficiency can also associate with angioedema a condition that causes sudden swelling in the deeper layers of skin and tissues. Understanding the pathways and the proteins involved in plasminogen's function presents therapeutic potential in managing these disorders.
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Sandwich ELISA - Human Plasminogen ELISA Kit (PLG) (ab108893)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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