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AB108893

Human Plasminogen ELISA Kit (PLG)

5

(2 Reviews)

|

(3 Publications)

Human Plasminogen ELISA Kit (PLG) is a sandwich ELISA designed to quantify Human Plasminogen (PLG) with a sensitivity of 0.29 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.625 - 40 ng/mL

View Alternative Names

Plasminogen, PLG

1 Images
Sandwich ELISA - Human Plasminogen ELISA Kit (PLG) (AB108893)
  • sELISA

Supplier Data

Sandwich ELISA - Human Plasminogen ELISA Kit (PLG) (AB108893)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum, Cerebral Spinal Fluid, Milk, Saliva

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.29 ng/mL

Range

0.625 - 40 ng/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

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Product details

Human Plasminogen ELISA Kit (PLG) ab108893 is a sandwich ELISA to measure Human Plasminogen (PLG) in serum, plasma, cell culture supernatant, cerebral spinal fluid (CSF), urine, milk, cell lysate, tissue with a sensitivity of 0.17 ng/ml.

How the assay works

A Plasminogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Plasminogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Plasminogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Plasminogen ELISA Kit (PLG) ab108893 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature.
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin Peroxidase Conjugate. Incubate at room temperature.
4. Add Chromogen Substrate to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.

Get results in 90 minutes with our SimpleStep ELISA® kit

Human Plasminogen ELISA Kit (ab196262)
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plasminogen also known as PLG is an important protein with a molecular weight of approximately 92 kDa. Plasminogen is a zymogen which means it is an inactive precursor that requires activation to function. It is produced primarily in the liver and circulates in blood plasma. Its expression occurs mainly in the liver but you can detect it in various tissues including the kidney. Plasminogen binds to tissue surfaces and undergoes transformation into plasmin an active enzyme that plays significant roles in many biological processes.
Biological function summary

Plasminogen plays a central role in fibrinolysis which is the breakdown of fibrin clots. This process is essential in wound healing and maintaining normal blood flow. Plasminogen once activated to plasmin acts by degrading fibrin a major component of blood clots. The protein forms part of the fibrinolytic system along with other components like tissue plasminogen activator (tPA). Together they ensure that any clots forming within blood vessels are regulated and dissolved as necessary preventing blockages.

Pathways

Plasminogen is deeply involved in the coagulation and fibrinolytic systems. The conversion of plasminogen to plasmin is a critical step in these pathways requiring activators such as urokinase. Plasminogen has interactions with proteins like alpha-2-antiplasmin and thrombin which help modulate its activity. These interactions ensure a careful balance is maintained between clot formation and dissolution which is vital for normal bodily function.

Plasminogen deficiency is linked with conditions like ligneous conjunctivitis a rare disorder characterized by membranous lesions mainly in the eyes. Reduced plasminogen activity can lead to excessive clot formation contributing to thrombosis risk. Plasminogen's interaction with plasmin and its deficiency can also associate with angioedema a condition that causes sudden swelling in the deeper layers of skin and tissues. Understanding the pathways and the proteins involved in plasminogen's function presents therapeutic potential in managing these disorders.
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Product protocols

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Target data

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1, C4 and C5 (PubMed : 6447255). Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.. (Microbial infection) ENO/enoloase from parasite P.falciparum (strain NF54) interacts with PLG present in the mosquito blood meal to promote the invasion of the mosquito midgut by the parasite ookinete (PubMed : 21949403). The catalytic active form, plasmin, is essential for the invasion of the mosquito midgut (PubMed : 21949403).. (Microbial infection) Binds to OspC on the surface of B.burgdorferi cells, possibly conferring an extracellular protease activity on the bacteria that allows it to traverse host tissue.. (Microbial infection) Interacts with dengue virus type 2 particles (PubMed : 31726374). Enhances dengue virus type 2 infection in Aedes aegypti mosquito midgut by increasing midgut internalization, resulting in higher infection rates and viral dissemination in mosquitoes (PubMed : 31726374).
See full target information PLG
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of hematology 116:937-946 PubMed35994163

2022

Plasma biomarkers associated with survival and thrombosis in hospitalized COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

David Cabrera-Garcia,Andrea Miltiades,Peter Yim,Samantha Parsons,Katerina Elisman,Mohammad Taghi Mansouri,Gebhard Wagener,Neil L Harrison

Transplantation 103:1821-1833 PubMed30964836

2019

Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Laura J Zitur,Peter J Chlebeck,Scott K Odorico,Juan S Danobeitia,Tiffany J Zens,Cees Van Kooten,Michael Eerhart,Jose A Reyes,Megan L Springer,Jennifer M Coonen,Kevin G Brunner,Saverio V Capuano,Anthony M D'Alessandro,Luis A Fernandez

PloS one 9:e105365 PubMed25133674

2014

A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM).

Applications

sELISA

Species

Human

Nessa Banville,Janette K Burgess,Jade Jaffar,Gavin Tjin,Luca Richeldi,Stefania Cerri,Elisa Persiani,Judith L Black,Brian G Oliver
View all publications
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Product promise

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