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AB108851

Human Platelet Glycoprotein IIb + IIIa ELISA Kit

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(6 Publications)

Human Platelet Glycoprotein IIb + IIIa ELISA Kit is a Sandwich ELISA for the measurement of Human Platelet Glycoprotein IIb + IIIa in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

CD41, GP2B, ITGAB, ITGA2B, Integrin alpha-IIb, GPalpha IIb, Platelet membrane glycoprotein IIb, GPIIb, CD61, GP3A, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa

1 Images
Sandwich ELISA - Human Platelet Glycoprotein IIb + IIIa ELISA Kit (AB108851)
  • sELISA

Supplier Data

Sandwich ELISA - Human Platelet Glycoprotein IIb + IIIa ELISA Kit (AB108851)

Representative Standard Curve using ab108851.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Platelets

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 19 pg/mL

Range

0.039 - 160 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Platelet Glycoprotein IIb + IIIa Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Platelet Glycoprotein IIb+IIIa concentrations in Human platelet-rich plasma and platelets.

A Platelet Glycoprotein llb + llla specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Platelet Glycoprotein llb+llla specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Platelet Glycoprotein llb + llla captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108851-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108851-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108851-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Platelet Glycoprotein IIb + IIIa Standard", "number":"AB108851-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Platelet Glycoprotein IIb + IIIa Microplate (12 x 8 well strips)", "number":"AB108851-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108851-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108851-CMP07", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Platelet Glycoprotein IIb + IIIa Antibody", "number":"AB108851-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108851-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108851-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Platelet glycoprotein IIb/IIIa also known as GP IIb/IIIa GP IIb/IIIa complex or integrin αIIbβ3 is an important protein integrin present on the surface of platelets. It has a molecular mass of approximately 130 kDa for each subunit and is expressed mainly in platelets and megakaryocytes. The protein functions as a receptor complex essential for platelet aggregation. Upon activation it changes conformation enabling it to bind fibrinogen and other adhesion molecules facilitating the cross-linking of platelets and forming a platelet plug.
Biological function summary

Platelet glycoprotein IIb/IIIa mediates the final common pathway for platelet aggregation by forming part of the integrin superfamily complex acting as a bridge between adjacent platelets. It converts mechanical stimuli into chemical signals important for thrombus formation. Interactions with another key platelet glycoprotein GP Ib-IX-V strengthen the adhesion and aggregation process ensuring an efficient hemostatic response to vascular injury.

Pathways

Platelet glycoprotein IIb/IIIa plays a significant role in the hemostasis and thrombosis pathways important for maintaining blood flow control. The protein is involved in mediating the platelet activation pathway with interactions notably including the von Willebrand factor and fibrinogen. This integrin bridges the interaction between these pathways which also involves associated molecules such as thrombin and thromboxane A2 playing a comprehensive role in clot formation.

Platelet glycoprotein IIb/IIIa is directly implicated in conditions such as acute coronary syndromes and stroke. Abnormalities or overactivity in this integrin can lead to excessive platelet aggregation and thrombosis which are pathological states in these diseases. Therapeutic antibodies targeting GP IIb/IIIa such as abciximab and other antagonists are often used to reduce risks associated with these disorders by preventing excessive clot formation. Additionally it relates closely to GP Ib-IX-V in pathological contexts influencing the development of thrombotic disorders.
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Product protocols

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Target data

Integrin alpha-IIb/beta-3 (ITGA2B : ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed : 9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface (By similarity). Integrin ITGA2B : ITGB3 is also the receptor of erythrocyte-specific ICAM4 ligand involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets (PubMed : 12477717).
See full target information ITGA2B

Additional targets

ITGB3
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical medicine 8: PubMed31783601

2019

Biomarkers of Vascular Injury and Type 2 Diabetes: A Prospective Study, Systematic Review and Meta-Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Pletsch-Borba,Cora Watzinger,Renée Turzanski Fortner,Verena Katzke,Lukas Schwingshackl,Solomon A Sowah,Anika Hüsing,Theron Johnson,Marie-Luise Groß,Sandra González Maldonado,Michael Hoffmeister,Peter Bugert,Rudolf Kaaks,Mirja Grafetstätter,Tilman Kühn

Nutrients 11: PubMed31484340

2019

Thrombomodulin and Thrombopoietin, Two Biomarkers of Hemostasis, Are Positively Associated with Adherence to the World Cancer Research Fund/American Institute for Cancer Research Recommendations for Cancer Prevention in a Population-Based Cross-Sectional Study.

Applications

Unspecified application

Species

Unspecified reactive species

Mirja Grafetstätter,Laura Pletsch-Borba,Disorn Sookthai,Nena Karavasiloglou,Theron Johnson,Verena A Katzke,Michael Hoffmeister,Peter Bugert,Rudolf Kaaks,Tilman Kühn

Cancer epidemiology, biomarkers & prevention : a p 28:1221-1227 PubMed31015200

2019

Plasma Fibrinogen and sP-Selectin are Associated with the Risk of Lung Cancer in a Prospective Study.

Applications

Unspecified application

Species

Unspecified reactive species

Mirja Grafetstätter,Anika Hüsing,Sandra González Maldonado,Disorn Sookthai,Theron Johnson,Laura Pletsch-Borba,Verena A Katzke,Michael Hoffmeister,Peter Bugert,Rudolf Kaaks,Tilman Kühn

Scientific reports 9:3004 PubMed30816120

2019

Biomarkers of vascular injury in relation to myocardial infarction risk: A population-based study.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Pletsch-Borba,Mirja Grafetstätter,Anika Hüsing,Sandra González Maldonado,Manja Kloss,Marie-Luise Groß,Theron Johnson,Disorn Sookthai,Peter Bugert,Rudolf Kaaks,Tilman Kühn

International journal of cancer 143:2659-2667 PubMed29923180

2018

Pre-diagnostic plasma concentrations of Fibrinogen, sGPIIb/IIIa, sP-selectin, sThrombomodulin, Thrombopoietin in relation to cancer risk: Findings from a large prospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Mirja E Graf,Disorn Sookthai,Theron Johnson,Ruth Schübel,Sandra González Maldonado,Laura Pletsch-Borba,Verena Katzke,Peter Bugert,Michael Hoffmeister,Rudolf Kaaks,Tilman Kühn

Clinical biochemistry 50:942-946 PubMed28571971

2017

Biological reproducibility of circulating P-Selectin, Thrombopoietin, GPIIb/IIIa and Thrombomodulin over one year.

Applications

ELISA

Species

Human

Mirja E Graf,Disorn Sookthai,Theron Johnson,Ruth Schübel,Verena Katzke,Peter Bugert,Michael Hoffmeister,Rudolf Kaaks,Tilman Kühn
View all publications
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