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AB316255

Human Plectin ELISA Kit

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Human Plectin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Plectin with a sensitivity of 5.303 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

PLEC1, PLEC, Plectin, PCN, PLTN, Hemidesmosomal protein 1, Plectin-1, HD1

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Sandwich ELISA - Human Plectin ELISA Kit (AB316255)
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Supplier Data

Sandwich ELISA - Human Plectin ELISA Kit (AB316255)

Example of human Plectin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Plectin ELISA Kit (AB316255)
  • sELISA

Supplier Data

Sandwich ELISA - Human Plectin ELISA Kit (AB316255)

Interpolated concentration of native Plectin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : mouse lung tissue extract 400 µg/mL, NIH/3T3 cell extract 6.25 µg/mL, rat lung tissue extract 1,000 µg/mL, and C6 cell extract 12.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Plectin ELISA Kit (AB316255)
  • sELISA

Supplier Data

Sandwich ELISA - Human Plectin ELISA Kit (AB316255)

Interpolated concentration of native Plectin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : U-2 OS cell extract 12.5 µg/mL, human colon tissue extract 60 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.303 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB32528

Anti-Plectin antibody [E398P]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] (AB32528)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Plectin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Plectin protein in Cell Lysate, Tissue extracts. Quantitate Human Plectin with 5.303 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "112 - 117 %", "average": "= 114" }, { "sample": "Tissue Extracts", "range": "90 - 105 %", "average": "= 100" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plectin also known as PLEC is a large protein with a molecular mass of approximately 500 kDa. It serves as a cross-linking element that plays an important role in maintaining the structural integrity of various cells. Plectin is widely expressed in tissues such as muscle skin and nervous tissue. Its mechanical function involves linking intermediate filaments to membrane complexes and other cytoskeletal structures which supports cellular architecture and stability.
Biological function summary

Plectin contributes significantly to the structural organization of cells and tissues. It is a part of the cytoskeletal complex and assists in cellular integrity by interacting with actin microfilaments microtubules and intermediate filaments. Plectin provides elasticity to cells allowing them to withstand mechanical stress. It ensures proper cellular functions and structural dynamics making it indispensable for maintaining tissue homeostasis.

Pathways

Plectin integrates into cellular signaling networks that control cytoskeletal organization and cell-matrix interactions. It plays a role in the integrin-mediated signaling pathway facilitating communication between the extracellular matrix and the cytoskeleton. Plectin interacts with proteins such as dystrophin which is involved in muscular pathways contributing to cell adhesion and motility.

Mutations or defects in the plectin gene can lead to conditions like epidermolysis bullosa simplex and muscular dystrophies. These disorders illustrate the important role that plectin plays in maintaining structural cell stability. In muscular dystrophies the interaction between plectin and dystrophin gets disrupted affecting muscle fiber integrity and leading to muscle weakness or degeneration. Such associations highlight plectin's significance in pathophysiological contexts.
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Product protocols

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Target data

Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.
See full target information PLEC
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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