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AB313925

Human Plexin B2/MM1 ELISA Kit

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Human Plexin B2/MM1 ELISA Kit is a Sandwich ELISA for the measurement of Human Plexin B2/MM1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

KIAA0315, PLXNB2, Plexin-B2, MM1

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Sandwich ELISA - Human Plexin B2/MM1 ELISA Kit (AB313925)
  • sELISA

Supplier Data

Sandwich ELISA - Human Plexin B2/MM1 ELISA Kit (AB313925)

Example of standard curve using ab313925. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.05 ng/mL

Range

0.05 - 12 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB193355

Anti-Plexin B2/MM1 antibody [EPR9965]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Plexin B2/MM1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Plexin B2/MM1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Plexin B2/MM1 coated on a 96- well plate.
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Recovery

[ { "sample": "Serum", "range": "92 - 127 %", "average": "= 109.4" }, { "sample": "Plasma", "range": "71 - 95 %", "average": "= 78.96" }, { "sample": "Cell culture media", "range": "68 - 85 %", "average": "= 75.51" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313925-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human Plexin B2/MM1 Antibody-coated ELISA Plate", "number":"AB313925-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313925-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Plexin B2/MM1 Protein Standard", "number":"AB313925-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Plexin B2/MM1 Detection Antibody", "number":"AB313925-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313925-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB313925-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313925-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313925-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313925-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plexin B2 also known as PLXNB2 or MM1 is a high-molecular-weight protein approximately 220 kDa found in humans. Plexin B2 is involved in the semaphorin receptor complex and is made of multiple domains that interact with semaphorin molecules to mediate cellular functions. Plexin B2 is expressed in various tissues including the brain spinal cord and bones indicating its role in different neural and structural processes. This protein interacts with other members of the Plexin family and undergoes changes that allow it to serve numerous signaling roles in the body.
Biological function summary

Plexin B2 plays a role in cellular signaling processes that are essential for neural patterning and tissue morphogenesis. It binds with semaphorins particularly Sema4C to guide axonal repulsion in neural development. Plexin B2 forms part of a receptor complex that helps cells interpret external semaphorin signals influencing cell movement and positioning. These interactions contribute to the remodeling of neural circuits which is important during both prenatal development and adult neural function.

Pathways

Plexin B2 participates in pathways engaging Rho GTPases which are important for cytoskeletal organization and cell movement. Plexin B2 influences the RhoA and Rac1 pathways impacting actin cytoskeleton dynamics and cell adhesion processes. These pathways where Plexin B2 acts with other Rho family proteins help transmit the changes induced by semaphorin binding altering cellular orientation and migration.

Plexin B2 has connections to neural tube defects and certain cancers. Defective Plexin B2 signaling can lead to improper neural tube closure contributing to conditions like spina bifida. Additionally in oncology the dysregulation of Plexin B2 expression or function links to tumor invasiveness and metastasis where it might interact with proteins like MET and Rho GTPases to influence cancer cell behavior. Understanding its role in these disorders aids in developing therapeutic strategies targeting aberrant Plexin B2 signaling.
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Product protocols

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Target data

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling (By similarity). Plays a role in glutamatergic synapse development and is required for SEMA4A-mediated excitatory synapse development (By similarity). Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248' (By similarity). Also acts as a cell surface receptor for angiogenin (ANG); promoting ANG endocytosis and translocation to the cytoplasm or nucleus (PubMed : 29100074, PubMed : 32510170). Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development (By similarity). Regulates the migration of cerebellar granule cells in the developing brain (By similarity). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (PubMed : 12183458). Plays a role in axon guidance, invasive growth and cell migration (PubMed : 15184888). May modulate the activity of RAC1 and CDC42 (By similarity).
See full target information PLXNB2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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