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AB313926

Human Plexin D1 ELISA Kit

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Human Plexin D1 ELISA Kit is a Sandwich ELISA for the measurement of Human Plexin D1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

KIAA0620, PLXND1, Plexin-D1

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Sandwich ELISA - Human Plexin D1 ELISA Kit (AB313926)
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Supplier Data

Sandwich ELISA - Human Plexin D1 ELISA Kit (AB313926)

Example of standard curve using ab313926. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.28 ng/mL

Range

0.28 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB28762

Anti-Plexin D1 antibody

Flow Cytometry (Intracellular) - Anti-Plexin D1 antibody (AB28762)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Plexin D1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Plexin D1 in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended as anticoagulants for use in this assay) and cell culture supernatants. This assay employs an antibody specific for human Plexin D1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human AMIGO, Aminopeptidase LRAP (ERAP2), Amnionless, Arylsulfatase A, Bcl-w, CD109, CD157, CD34, CD83, CLEC1, CLEC10A (CD301), CMG-2, CREG, Cystatin SN, Cytokeratin-8, Dectin-1 (CLEC7A), Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin (CD207), Lumican, Matriptase (ST14), MEP1B, Nectin-3, OX40 (TNFRSF4), OX40 Ligand (TNFSF4), p27, Pappalysin2, Plexin B3, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R (TNFRSF12), ULBP-3.

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Recovery

[ { "sample": "Serum", "range": "71 - 92 %", "average": "= 77.98" }, { "sample": "Plasma", "range": "75 - 109 %", "average": "= 87.49" }, { "sample": "Cell culture media", "range": "88 - 95 %", "average": "= 91.29" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313926-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313926-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313926-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Plexin D1 Detection Antibody", "number":"AB313926-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB313926-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313926-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313926-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313926-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Plexin D1 Protein Standard", "number":"AB313926-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Plexin D1 Antibody-coated ELISA Plate", "number":"AB313926-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB313926-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Plexin D1 also known as PLXND1 is a transmembrane receptor that plays an important role in cell signaling. It belongs to the plexin family and has a molecular weight of roughly 250 kDa. The plexin D1 receptor is widely expressed in various tissues including neurons endothelial cells and the cardiovascular system. It is an integral part of the semaphorin signaling pathway interacting primarily with class 3 semaphorins and influencing cytoskeletal dynamics.
Biological function summary

Plexin D1 regulates cell movement and positioning by modulating cell adhesion and cytoskeletal rearrangement. As part of semaphorin signaling plexin D1 coordinates with other membrane proteins and signaling molecules to control processes like axon guidance and vascular patterning. It does not act alone but rather within a complex network of associated proteins that enhance its functional outcomes. Its involvement is important at the developmental stages especially within the nervous and vascular systems.

Pathways

The semaphorin-plexin signaling axis is where plexin D1 finds its significant role. It impacts the MAPK and PI3K pathways which are essential in controlling cellular responses to environmental cues. In these pathways it interacts with proteins such as Ras and Rho GTPases contributing to the regulation of cellular dynamics and survival. The interplay of plexin D1 with semaphorin 3E is particularly important for guiding cells in response to repulsive cues within the cellular environment.

Plexin D1 is associated with cancer and vascular disorders. Its role in cellular migration and angiogenesis makes it an important player in tumor progression and metastasis. Abnormal plexin D1 signaling might lead to improper blood vessel formation contributing to diseases like heart defects. In cancer its interaction with proteins like VEGF can promote tumor-induced angiogenesis exacerbating the disease state. Understanding these interactions is fundamental for developing therapeutic interventions targeting plexin D1-mediated pathways.
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Product protocols

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Target data

Cell surface receptor for SEMA4A and for class 3 semaphorins, such as SEMA3A, SEMA3C and SEMA3E. Plays an important role in cell-cell signaling, and in regulating the migration of a wide spectrum of cell types. Regulates the migration of thymocytes in the medulla. Regulates endothelial cell migration. Plays an important role in ensuring the specificity of synapse formation. Required for normal development of the heart and vasculature (By similarity). Mediates anti-angiogenic signaling in response to SEMA3E.
See full target information PLXND1
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Product promise

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