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AB100629

Human PLGF ELISA Kit

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(7 Publications)

Human PLGF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PLGF in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PGFL, PLGF, PGF, Placenta growth factor, PlGF

1 Images
Sandwich ELISA - Human PLGF ELISA Kit (AB100629)
  • sELISA

Supplier Data

Sandwich ELISA - Human PLGF ELISA Kit (AB100629)

Representative Standard Curve using ab100629.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

1.372 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's PLGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PLGF in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human PLGF coated on a 96-well plate. Standards and samples are pipetted into the wells and PLGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PLGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "75 - 115 %", "average": "= 103.4" }, { "sample": "Serum", "range": "79 - 96 %", "average": "= 88.09" }, { "sample": "Plasma", "range": "68 - 93 %", "average": "= 75.69" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100629-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100629-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100629-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100629-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human PLGF", "number":"AB100629-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PLGF Microplate (12 x 8 wells)", "number":"AB100629-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100629-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100629-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human PLGF Standard (lyophilized)", "number":"AB100629-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
See full target information PGF
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed35883694

2022

Maternal Fibroblast Growth Factor 21 Levels Decrease during Early Pregnancy in Normotensive Pregnant Women but Are Higher in Preeclamptic Women-A Longitudinal Study.

Applications

Unspecified application

Species

Unspecified reactive species

Julieth Daniela Buell-Acosta,Maria Fernanda Garces,Arturo José Parada-Baños,Edith Angel-Muller,Maria Carolina Paez,Javier Eslava-Schmalbach,Franklin Escobar-Cordoba,Sofia Alexandra Caminos-Cepeda,Ezequiel Lacunza,Justo P Castaño,Rubén Nogueiras,Carlos Dieguez,Ariel Iván Ruiz-Parra,Jorge Eduardo Caminos

Bioengineered 13:4939-4950 PubMed35156527

2022

Quantitative proteomics reveal three potential biomarkers for risk assessment of acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Xie,Hongzhou Zhang,Tieqiu Huang

Kidney international 99:646-656 PubMed33144212

2020

Mechanisms of vascular dysfunction in the interleukin-10-deficient murine model of preeclampsia indicate nitric oxide dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hajrunisa Cubro,Karl A Nath,Sonja Suvakov,Oscar Garcia-Valencia,Santosh Parashuram,Wendy M White,Tracey L Weissgerber,Meryl C Nath,Natasa M Milic,Fernando Sontag,Livius V d'Uscio,Yi Zhu,James L Kirkland,Tamar Tchkonia,Mariam P Alexander,Reade A Quinton,Zvonimir S Katusic,Joseph P Grande,Vesna D Garovic

Placenta 90:118-120 PubMed32056542

2020

Altered level of salivary placental growth factor is associated with preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Shannon L Rao,Ada Taymoori,David T W Wong,Jill L Maron

Cancer letters 460:29-40 PubMed31173855

2019

Sublethal heat treatment of hepatocellular carcinoma promotes intrahepatic metastasis and stemness in a VEGFR1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Li Tan,Shuling Chen,Guangyan Wei,Yue Li,Junbin Liao,Huilin Jin,Ying Zou,Manling Huang,Zhenwei Peng,Yu Guo,Sui Peng,Lixia Xu,Ming Kuang

Stem cells international 2018:1310904 PubMed30675166

2018

Efficient Nonviral Transfection of Human Bone Marrow Mesenchymal Stromal Cells Shown Using Placental Growth Factor Overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Winston Y Cheung,Owen Hovey,Jonathan M Gobin,Gauri Muradia,Jelica Mehic,Carole Westwood,Jessie R Lavoie

PloS one 10:e0131013 PubMed26121675

2015

Maternal Serum Meteorin Levels and the Risk of Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

María F Garcés,Elizabeth Sanchez,Luisa F Cardona,Elkin L Simanca,Iván González,Luis G Leal,José A Mora,Andrés Bedoya,Juan P Alzate,Ángel Y Sánchez,Javier H Eslava-Schmalbach,Roberto Franco-Vega,Mario O Parra,Ariel I Ruíz-Parra,Carlos Diéguez,Rubén Nogueiras,Jorge E Caminos
View all publications
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