Human PLGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PLGF with a sensitivity of 4.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.63 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.4 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
Alternative names
PGFL, PLGF, PGF, Placenta growth factor, PlGF
What's included?
Recommended products
Human PLGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PLGF with a sensitivity of 4.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.63 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 113 | Range 112 - 114 % |
Sample type Serum | Average % = 86 | Range 85 - 88 % |
Sample type EDTA Plasma | Average % = 99 | Range 96 - 103 % |
Sample type Urine | Average % = 86 | Range 83 - 91 % |
Sample type Cell culture media | Average % = 98 | Range 93 - 101 % |
Sample type Heparin Plasma | Average % = 102 | Range 98 - 105 % |
Sample type Citrate plasma | Average % = 93 | Range 92 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human PLGF ELISA Kit (ab260056) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PLGF protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PLGF with 4.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
PLGF (placenta growth factor) is a secreted growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. Four PLGF isoforms are produced by alternative splicing, three of them are present in placental tissue. Only PLGF-1 is found in the colon and mammary carcinomas. PLGF-3 binds to the receptor FLT1/VEGFR-1. Isoform PLGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
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3 product images
Sandwich ELISA - Human PLGF ELISA Kit (ab260056)
The PIGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PLGF ELISA Kit (ab260056)
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PIGF concentration was determined to be 38.42 pg/mL in detectable samples with a range of ND - 44.05 pg/mL for all samples.
Sandwich ELISA - Human PLGF ELISA Kit (ab260056)
The concentrations of PIGF were measured in duplicates, interpolated from the PIGF standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant serum 50% and JAR cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PIGF concentration was determined to be 1,147 pg/mL in neat pregnant serum and 11,000 pg/mL in neat JAR cell culture supernatant.
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