Human Plunc ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Plunc in Human in Plasma, Cell culture supernatant, Serum samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 40.96 - 10000 pg/mL
- Sensitivity
- = 40 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Lipid-binding protein which shows high specificity for the surfactant phospholipid dipalmitoylphosphatidylcholine (DPPC) (PubMed:25223608). Plays a role in the innate immune responses of the upper airways (PubMed:23132494, PubMed:23499554). Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae (PubMed:23132494, PubMed:23499554, PubMed:27145151). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus (PubMed:24043776, PubMed:24124190). Plays a role in the airway inflammatory response after exposure to irritants (PubMed:11425234). May attract macrophages and neutrophils (PubMed:23132494).
Alternative names
LUNX, NASG, PLUNC, SPLUNC1, SPURT, UNQ787/PRO1606, BPIFA1, BPI fold-containing family A member 1, Lung-specific protein X, Nasopharyngeal carcinoma-related protein, Palate lung and nasal epithelium clone protein, Secretory protein in upper respiratory tracts, Short PLUNC1, Tracheal epithelium-enriched protein, Von Ebner protein Hl
What's included?
Recommended products
Human Plunc ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Plunc in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 40.96 - 10000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 40 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 142.7 | Range 138 - 145 % |
Sample type Plasma | Average % = 128.4 | Range 76 - 147 % |
Sample type Cell culture media | Average % = 119.2 | Range 87 - 137 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Plunc ELISA Kit (ab277427) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Plunc in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Plunc coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Plunc present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Plunc antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Plunc bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Palate Lung Nasal Epithelium Clone (PLUNC) also known as SPLUNC1 or BPI-fold containing family A member 1 is a protein involved in host defense mechanisms. It has a molecular mass of approximately 25 kDa. High expression levels are found in the upper respiratory tract where it plays a role in airway surface liquid homeostasis. You can also detect it in salivary glands and other mucosal surfaces. Its expression is linked to innate immunity providing a first line of defense.
- Biological function summary
This protein functions to modulate the physical and chemical environment at mucosal surfaces. It helps maintain the integrity of epithelial barriers against pathogens. As part of no particular protein complex PLUNC acts as a surfactant with anti-microbial properties in respiratory tissues. It helps maintain the viscosity and hydration of airway secretions facilitating the trapping and clearance of inhaled pathogens and particles.
- Pathways
PLUNC is implicated in innate immune responses and mucosal defense mechanisms. It interacts within pathways involving the regulation of inflammation and mucociliary clearance. Proteins related to PLUNC in these pathways include SBEM and the airway-specific secretory proteins. Through these interactions it helps the lungs manage both pathogen load and airway clearances such as mucociliary escalator actions necessary to protect the respiratory system.
- Associated diseases and disorders
PLUNC is related to cystic fibrosis and chronic rhinosinusitis. In cystic fibrosis its regulation affects mucus viscosity and bacterial colonization potentially worsening respiratory symptoms. In chronic rhinosinusitis altered expression of PLUNC links to inflammation and recurrent infections. It connects with CFTR protein in cystic fibrosis impacting ion transport and mucociliary function. Through these mechanisms PLUNC can play a significant role in the progression of specific airway-related conditions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
ELISA - Human Plunc ELISA Kit (ab277427)
These standard curves are for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com