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AB277427

Human Plunc ELISA Kit

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Human Plunc ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Plunc in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

LUNX, NASG, PLUNC, SPLUNC1, SPURT, UNQ787/PRO1606, BPIFA1, BPI fold-containing family A member 1, Lung-specific protein X, Nasopharyngeal carcinoma-related protein, Palate lung and nasal epithelium clone protein, Secretory protein in upper respiratory tracts, Short PLUNC1, Tracheal epithelium-enriched protein, Von Ebner protein Hl

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ELISA - Human Plunc ELISA Kit (AB277427)
  • ELISA

Supplier Data

ELISA - Human Plunc ELISA Kit (AB277427)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 40 pg/mL

Range

40.96 - 10000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Plunc ELISA Kit (ab277427) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Plunc in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Plunc coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Plunc present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Plunc antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Plunc bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "138 - 145 %", "average": "= 142.7" }, { "sample": "Plasma", "range": "76 - 147 %", "average": "= 128.4" }, { "sample": "Cell culture media", "range": "87 - 137 %", "average": "= 119.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277427-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277427-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277427-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277427-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Plunc coated Microplate", "number":"AB277427-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Plunc standard protein (Lyophilized)", "number":"AB277427-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277427-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"550X HRP-Streptavidin Concentrate", "number":"AB277427-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Plunc Antibody", "number":"AB277427-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Palate Lung Nasal Epithelium Clone (PLUNC) also known as SPLUNC1 or BPI-fold containing family A member 1 is a protein involved in host defense mechanisms. It has a molecular mass of approximately 25 kDa. High expression levels are found in the upper respiratory tract where it plays a role in airway surface liquid homeostasis. You can also detect it in salivary glands and other mucosal surfaces. Its expression is linked to innate immunity providing a first line of defense.
Biological function summary

This protein functions to modulate the physical and chemical environment at mucosal surfaces. It helps maintain the integrity of epithelial barriers against pathogens. As part of no particular protein complex PLUNC acts as a surfactant with anti-microbial properties in respiratory tissues. It helps maintain the viscosity and hydration of airway secretions facilitating the trapping and clearance of inhaled pathogens and particles.

Pathways

PLUNC is implicated in innate immune responses and mucosal defense mechanisms. It interacts within pathways involving the regulation of inflammation and mucociliary clearance. Proteins related to PLUNC in these pathways include SBEM and the airway-specific secretory proteins. Through these interactions it helps the lungs manage both pathogen load and airway clearances such as mucociliary escalator actions necessary to protect the respiratory system.

PLUNC is related to cystic fibrosis and chronic rhinosinusitis. In cystic fibrosis its regulation affects mucus viscosity and bacterial colonization potentially worsening respiratory symptoms. In chronic rhinosinusitis altered expression of PLUNC links to inflammation and recurrent infections. It connects with CFTR protein in cystic fibrosis impacting ion transport and mucociliary function. Through these mechanisms PLUNC can play a significant role in the progression of specific airway-related conditions.
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Product protocols

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Target data

Lipid-binding protein which shows high specificity for the surfactant phospholipid dipalmitoylphosphatidylcholine (DPPC) (PubMed : 25223608). Plays a role in the innate immune responses of the upper airways (PubMed : 23132494, PubMed : 23499554). Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae (PubMed : 23132494, PubMed : 23499554, PubMed : 27145151). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus (PubMed : 24043776, PubMed : 24124190). Plays a role in the airway inflammatory response after exposure to irritants (PubMed : 11425234). May attract macrophages and neutrophils (PubMed : 23132494).
See full target information BPIFA1
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