AB260069

Human PNP ELISA Kit (Purine Nucleoside Phosphorylase)

Recombinant
SimpleStep
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Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) is a single-wash 90-min Simplestep used to quantify Human PNP (Purine Nucleoside Phosphorylase) with a sensitivity of 40 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

NP, PNP, Purine nucleoside phosphorylase, Inosine phosphorylase, Inosine-guanosine phosphorylase

2 Images

Sandwich ELISA - Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (AB260069)

sELISA

Supplier Data

Sandwich ELISA - Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (AB260069)

Interpolated concentrations of native PNP in human serum and plasma samples.

The concentrations of PNP were measured in duplicate, interpolated from the PNP standard curves, and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (edta) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PNP concentration was determined to be 1435.64 pg/mL in serum, 4419.64 pg/mL in plasma (citrate), 2483.57 pg/mL in plasma (heparin), and 1750.71 pg/mL in plasma (edta).

sELISA

Supplier Data

Sandwich ELISA - Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (AB260069)

Example of human PNP standard curve in Sample Diluent NS.

The PNP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 40 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (ab260069) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PNP (Purine Nucleoside Phosphorylase) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PNP (Purine Nucleoside Phosphorylase) with 40 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Purine Nucleoside Phosphorylase (PNP) is a catabolic enzyme which breaks down purine residues by releasing the nucleobase and phosphorylating the ribose residue. It is also used to produce nucleotide monobases as a salvage pathway. PNP plays an important role in the maintenance of T-cells. This kit targets the full-length protein.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" } ]

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Recovery

[ { "sample": "Serum", "range": "85 - 108 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "85 - 110 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "82 - 104 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "90 - 114 %", "average": "= 103" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoside phosphorylase also known as PNP or purine nucleoside phosphorylase is an enzyme with a vital mechanical role in nucleoside metabolism. The enzyme facilitates the phosphorolytic cleavage of the glycosidic bond in nucleosides producing ribose 1-phosphate and free bases such as guanine and hypoxanthine. This catalytic function plays an important part in nucleotide salvage pathways. PNP has a molecular mass of approximately 32000 Daltons and shows expression predominantly in the lymphoid tissues including the thymus and spleen.

Biological function summary

Nucleoside phosphorylase is important in maintaining nucleotide homeostasis. It operates as a monomer or part of a homotrimeric complex which allows it to efficiently catalyze its reactions in purine metabolism. The absence or dysfunction of PNP results in the accumulation of nucleosides and diminished levels of nucleotide pools which can severely hamper DNA replication and repair.

Pathways

Nucleoside phosphorylase plays an integral role in the purine salvage pathway which is vital for recycling purines to form new nucleotides. It works alongside other enzymes such as adenine phosphoribosyltransferase to conserve energy by recycling purines. This pathway connects closely with the hypoxanthine-guanine phosphoribosyltransferase (HGPRT) pathway showing how PNP is intertwined in broader nucleotide biosynthesis and degradation processes.

Deficiencies in nucleoside phosphorylase correlate strongly with immunodeficiencies notably purine nucleoside phosphorylase deficiency (PNP deficiency) which leads to compromised T-cell immunity. This condition can result in recurrent infections and developmental delay. Additionally the enzyme's dysregulation associates with certain leukemias where altered nucleotide pools contribute to the proliferation of malignant cells. In these contexts PNP interacts with proteins central to these disorders including those involved in purine metabolism placing it at a critical junction for potential therapeutic intervention.

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Product protocols

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Target data

Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate (PubMed : 23438750, PubMed : 9305964). Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine (PubMed : 9305964).

See full target information PNP

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human PNP ELISA Kit (Purine Nucleoside Phosphorylase)

Sandwich ELISA - Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (AB260069)

Sandwich ELISA - Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (AB260069)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Product promise